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Evaluation of interaction between imidazolium-based chloride ionic liquids and calf thymus DNA.

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Title: Evaluation of interaction between imidazolium-based chloride ionic liquids and calf thymus DNA.
Authors: Liu, Huijun1 lhj@zjgsu.edu.cn, Dong, Ying1, Wu, Jian1, Chen, Caidong1, Liu, Dingdong1, Zhang, Qi1, Du, Shaoting1 dushaoting@zjgsu.edu.cn
Source: Science of the Total Environment. Oct2016, Vol. 566, p1-7. 7p.
Subject Terms: Imidazoles, Ionic liquids, DNA, Ultraviolet-visible spectroscopy, Fluorescence spectroscopy, Hyperchromic effect, Quenching (Chemistry)
Abstract: With ionic liquids (ILs) being widely used, the toxicity of many ILs has been studied and verified. However the mechanism underlying the interaction between ILs and DNA needs to be investigated. In this study, the interaction of three imidazolium-based ILs ([C 8 mim]Cl, [C 12 mim]Cl, and [C 16 mim]Cl) with calf thymus DNA (ctDNA) was investigated by UV absorption spectroscopy and fluorescence spectroscopy. An intense interaction between [C n mim]Cl and ctDNA was observed, involving a hypochromic effect or even a hyperchromic effect, in the UV absorption spectrum of ctDNA at 260 nm. The T m of ctDNA increased over 10 °C after binding with [C n mim]Cl, and the K SV values of [C n mim]Cl-ctDNA quenched by potassium iodide (KI) were lower than those of [C n mim]Cl. The fluorescence intensity of ctDNA-ethidium bromide (EB) was gradually quenched as the [C n mim]Cl concentration increased. The results indicated that ctDNA interacted with [C n mim]Cl through an intercalation binding mode. The mechanism of fluorescence quenching of [C n mim]Cl with ctDNA involved static quenching. The binding constant between [C n mim]Cl and ctDNA were 1443, 11169, and 67189, and the number of binding sites were 0.89, 1.10, and 1.27 at 298 K, for [C 8 mim]Cl, [C 12 mim]Cl, and [C 16 mim]Cl, respectively. The results indicated that the intercalation binding between the three [C n mim]Cl and ctDNA increased with increasing IL-alkyl chain length. These results will aid in the understanding of the mechanism of toxicity and of the biologically mediated environmental processes of ILs. [ABSTRACT FROM AUTHOR]
Copyright of Science of the Total Environment is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
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  Data: Evaluation of interaction between imidazolium-based chloride ionic liquids and calf thymus DNA.
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  Data: <searchLink fieldCode="AR" term="%22Liu%2C+Huijun%22">Liu, Huijun</searchLink><relatesTo>1</relatesTo><i> lhj@zjgsu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Dong%2C+Ying%22">Dong, Ying</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Jian%22">Wu, Jian</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Caidong%22">Chen, Caidong</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Dingdong%22">Liu, Dingdong</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Qi%22">Zhang, Qi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Du%2C+Shaoting%22">Du, Shaoting</searchLink><relatesTo>1</relatesTo><i> dushaoting@zjgsu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Science+of+the+Total+Environment%22">Science of the Total Environment</searchLink>. Oct2016, Vol. 566, p1-7. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Imidazoles%22">Imidazoles</searchLink><br /><searchLink fieldCode="DE" term="%22Ionic+liquids%22">Ionic liquids</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Ultraviolet-visible+spectroscopy%22">Ultraviolet-visible spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+spectroscopy%22">Fluorescence spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Hyperchromic+effect%22">Hyperchromic effect</searchLink><br /><searchLink fieldCode="DE" term="%22Quenching+%28Chemistry%29%22">Quenching (Chemistry)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: With ionic liquids (ILs) being widely used, the toxicity of many ILs has been studied and verified. However the mechanism underlying the interaction between ILs and DNA needs to be investigated. In this study, the interaction of three imidazolium-based ILs ([C 8 mim]Cl, [C 12 mim]Cl, and [C 16 mim]Cl) with calf thymus DNA (ctDNA) was investigated by UV absorption spectroscopy and fluorescence spectroscopy. An intense interaction between [C n mim]Cl and ctDNA was observed, involving a hypochromic effect or even a hyperchromic effect, in the UV absorption spectrum of ctDNA at 260 nm. The T m of ctDNA increased over 10 °C after binding with [C n mim]Cl, and the K SV values of [C n mim]Cl-ctDNA quenched by potassium iodide (KI) were lower than those of [C n mim]Cl. The fluorescence intensity of ctDNA-ethidium bromide (EB) was gradually quenched as the [C n mim]Cl concentration increased. The results indicated that ctDNA interacted with [C n mim]Cl through an intercalation binding mode. The mechanism of fluorescence quenching of [C n mim]Cl with ctDNA involved static quenching. The binding constant between [C n mim]Cl and ctDNA were 1443, 11169, and 67189, and the number of binding sites were 0.89, 1.10, and 1.27 at 298 K, for [C 8 mim]Cl, [C 12 mim]Cl, and [C 16 mim]Cl, respectively. The results indicated that the intercalation binding between the three [C n mim]Cl and ctDNA increased with increasing IL-alkyl chain length. These results will aid in the understanding of the mechanism of toxicity and of the biologically mediated environmental processes of ILs. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Science of the Total Environment is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.scitotenv.2016.05.087
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 7
        StartPage: 1
    Subjects:
      – SubjectFull: Imidazoles
        Type: general
      – SubjectFull: Ionic liquids
        Type: general
      – SubjectFull: DNA
        Type: general
      – SubjectFull: Ultraviolet-visible spectroscopy
        Type: general
      – SubjectFull: Fluorescence spectroscopy
        Type: general
      – SubjectFull: Hyperchromic effect
        Type: general
      – SubjectFull: Quenching (Chemistry)
        Type: general
    Titles:
      – TitleFull: Evaluation of interaction between imidazolium-based chloride ionic liquids and calf thymus DNA.
        Type: main
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            NameFull: Liu, Huijun
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            NameFull: Dong, Ying
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            NameFull: Wu, Jian
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            NameFull: Chen, Caidong
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            NameFull: Liu, Dingdong
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            NameFull: Zhang, Qi
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            NameFull: Du, Shaoting
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            – D: 01
              M: 10
              Text: Oct2016
              Type: published
              Y: 2016
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              Value: 566
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