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ELF electromagnetic fields increase hydrogen peroxide (H2O2)-induced mutations in pTN89 plasmids

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Title: ELF electromagnetic fields increase hydrogen peroxide (H2O2)-induced mutations in pTN89 plasmids
Authors: Koyama, Shin1,2, Nakahara, Takehisa1, Hirose, Hideki3, Ding, Gui-Rong1, Takashima, Yoshio1, Isozumi, Yasuhito2, Miyakoshi, Junji1 miyakosh@cc.hirosaki-u.ac.jp
Source: Mutation Research - Genetic Toxicology & Environmental Mutagenesis. May2004, Vol. 560 Issue 1, p27. 6p.
Subject Terms: Plasmids, Hydrogen peroxide, Cytoplasmic inheritance, Genes
Abstract: We have examined the mutational effects of hydrogen peroxide (H2O2) in the presence and absence of an extremely low-frequency magnetic field (ELFMF), using pTN89 plasmids. Mutations were detected in the supF gene carried by these plasmids in Escherichia coli. The plasmids were either treated with H2O2 (1 μM) alone at 37 °C for 4 h, or were exposed to an ELFMF (60 Hz, 5 millitesla (mT)) simultaneously with H2O2 treatment. The mutation frequency was 2.28×10−4 for H2O2 treatment alone, and 5.81×10−4 for ELFMF exposure with H2O2 treatment. We did not observe any mutations using treatment with ELFMF exposure alone. This indicates that the ELFMF may potentiate H2O2-induced mutation. Sequence analysis of the supF mutant plasmids revealed that base substitutions, G:C→A:T transitions and G:C→T:A transversions were dominant in both treatment groups, and there was no difference in the mutation spectrum or the hotspots between the groups. Therefore, ELFMFs may interact and potentiate the damage induced by H2O2, resulting in an increase in the number of mutations. [Copyright &y& Elsevier]
Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: ELF electromagnetic fields increase hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>)-induced mutations in pTN89 plasmids
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Koyama%2C+Shin%22">Koyama, Shin</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Nakahara%2C+Takehisa%22">Nakahara, Takehisa</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hirose%2C+Hideki%22">Hirose, Hideki</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ding%2C+Gui-Rong%22">Ding, Gui-Rong</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Takashima%2C+Yoshio%22">Takashima, Yoshio</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Isozumi%2C+Yasuhito%22">Isozumi, Yasuhito</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Miyakoshi%2C+Junji%22">Miyakoshi, Junji</searchLink><relatesTo>1</relatesTo><i> miyakosh@cc.hirosaki-u.ac.jp</i>
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  Data: <searchLink fieldCode="JN" term="%22Mutation+Research+-+Genetic+Toxicology+%26+Environmental+Mutagenesis%22">Mutation Research - Genetic Toxicology & Environmental Mutagenesis</searchLink>. May2004, Vol. 560 Issue 1, p27. 6p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Plasmids%22">Plasmids</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrogen+peroxide%22">Hydrogen peroxide</searchLink><br /><searchLink fieldCode="DE" term="%22Cytoplasmic+inheritance%22">Cytoplasmic inheritance</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: We have examined the mutational effects of hydrogen peroxide (H2O2) in the presence and absence of an extremely low-frequency magnetic field (ELFMF), using pTN89 plasmids. Mutations were detected in the supF gene carried by these plasmids in Escherichia coli. The plasmids were either treated with H2O2 (1 μM) alone at 37 °C for 4 h, or were exposed to an ELFMF (60 Hz, 5 millitesla (mT)) simultaneously with H2O2 treatment. The mutation frequency was <F>2.28×10−4</F> for H2O2 treatment alone, and <F>5.81×10−4</F> for ELFMF exposure with H2O2 treatment. We did not observe any mutations using treatment with ELFMF exposure alone. This indicates that the ELFMF may potentiate H2O2-induced mutation. Sequence analysis of the supF mutant plasmids revealed that base substitutions, G:<F>C→A</F>:T transitions and G:<F>C→T</F>:A transversions were dominant in both treatment groups, and there was no difference in the mutation spectrum or the hotspots between the groups. Therefore, ELFMFs may interact and potentiate the damage induced by H2O2, resulting in an increase in the number of mutations. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.mrgentox.2004.01.012
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      – Code: eng
        Text: English
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        PageCount: 6
        StartPage: 27
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      – SubjectFull: Plasmids
        Type: general
      – SubjectFull: Hydrogen peroxide
        Type: general
      – SubjectFull: Cytoplasmic inheritance
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      – SubjectFull: Genes
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      – TitleFull: ELF electromagnetic fields increase hydrogen peroxide (H2O2)-induced mutations in pTN89 plasmids
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            NameFull: Koyama, Shin
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            NameFull: Nakahara, Takehisa
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            NameFull: Hirose, Hideki
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            NameFull: Takashima, Yoshio
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            NameFull: Isozumi, Yasuhito
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              M: 05
              Text: May2004
              Type: published
              Y: 2004
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