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Knockdown of the GABA receptor RDL genes decreases abamectin susceptibility in the rice stem borer, Chilo suppressalis.

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Title: Knockdown of the GABA receptor RDL genes decreases abamectin susceptibility in the rice stem borer, Chilo suppressalis.
Authors: Meng, Xiangkun1, Yang, Xuemei1, Zhang, Nan1, Jiang, Heng1, Ge, Huichen1, Chen, Minxuan1, Qian, Kun1, Wang, Jianjun1 wangjj@yzu.edu.cn
Source: Pesticide Biochemistry & Physiology. Jan2019, Vol. 153, p171-175. 5p.
Subject Terms: Aminobutyric acid, GABA, Neurotransmitter receptors, Chilo suppressalis, Gene expression
Abstract: Abstract The γ-aminobutyric acid (GABA) receptor is a primary neurotransmitter receptor in both vertebrate and invertebrate nervous systems. Multiple RDL subunits have been found in insects including the rice stem borer, Chilo suppressalis , however, comparative characterization of duplicated RDL genes in insects is still limited. In this study, comparison of the genomic sequences and the cDNA sequences revealed that both CsRDL1 and CsRDL2 consisted of 10 exons and 9 introns, and their exon-intron boundaries occur in the same position with respect to the coding sequences. Expression profiling showed that both CsRDL1 and CsRDL2 were predominantly expressed in nervous system, and had low expression levels in the gut and integument. The transcript level of CsRDL2 dramatically increased from the prepupae to late pupae and were much higher than that of CsRDL1 in adult stages. Notably, dietary ingestion of dsRDL1 and dsRDL2 significantly decreased the larval susceptibility to abamectin. These results suggest that CsRDL1 and CsRDL2 might play both physiological roles in development and toxicological roles in action of abamectin in C. suppressalis. Graphical abstract Unlabelled Image Highlights • The genomic sequences of CsRDL1 and CsRDL2 were compared. • CsRDL1 and CsRDL2 were predominantly expressed in nervous system. • The transcript level of CsRDL2 dramatically increased from the prepupae to late pupae. • Knockdown of dsRDL1 and dsRDL2 significantly decreased the larval susceptibility to abamectin. [ABSTRACT FROM AUTHOR]
Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Knockdown of the GABA receptor RDL genes decreases abamectin susceptibility in the rice stem borer, Chilo suppressalis.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Meng%2C+Xiangkun%22">Meng, Xiangkun</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yang%2C+Xuemei%22">Yang, Xuemei</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Nan%22">Zhang, Nan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jiang%2C+Heng%22">Jiang, Heng</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ge%2C+Huichen%22">Ge, Huichen</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Minxuan%22">Chen, Minxuan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Qian%2C+Kun%22">Qian, Kun</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Jianjun%22">Wang, Jianjun</searchLink><relatesTo>1</relatesTo><i> wangjj@yzu.edu.cn</i>
– Name: TitleSource
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  Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. Jan2019, Vol. 153, p171-175. 5p.
– Name: Subject
  Label: Subject Terms
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  Data: <searchLink fieldCode="DE" term="%22Aminobutyric+acid%22">Aminobutyric acid</searchLink><br /><searchLink fieldCode="DE" term="%22GABA%22">GABA</searchLink><br /><searchLink fieldCode="DE" term="%22Neurotransmitter+receptors%22">Neurotransmitter receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Chilo+suppressalis%22">Chilo suppressalis</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract The γ-aminobutyric acid (GABA) receptor is a primary neurotransmitter receptor in both vertebrate and invertebrate nervous systems. Multiple RDL subunits have been found in insects including the rice stem borer, Chilo suppressalis , however, comparative characterization of duplicated RDL genes in insects is still limited. In this study, comparison of the genomic sequences and the cDNA sequences revealed that both CsRDL1 and CsRDL2 consisted of 10 exons and 9 introns, and their exon-intron boundaries occur in the same position with respect to the coding sequences. Expression profiling showed that both CsRDL1 and CsRDL2 were predominantly expressed in nervous system, and had low expression levels in the gut and integument. The transcript level of CsRDL2 dramatically increased from the prepupae to late pupae and were much higher than that of CsRDL1 in adult stages. Notably, dietary ingestion of dsRDL1 and dsRDL2 significantly decreased the larval susceptibility to abamectin. These results suggest that CsRDL1 and CsRDL2 might play both physiological roles in development and toxicological roles in action of abamectin in C. suppressalis. Graphical abstract Unlabelled Image Highlights • The genomic sequences of CsRDL1 and CsRDL2 were compared. • CsRDL1 and CsRDL2 were predominantly expressed in nervous system. • The transcript level of CsRDL2 dramatically increased from the prepupae to late pupae. • Knockdown of dsRDL1 and dsRDL2 significantly decreased the larval susceptibility to abamectin. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.pestbp.2018.11.017
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 5
        StartPage: 171
    Subjects:
      – SubjectFull: Aminobutyric acid
        Type: general
      – SubjectFull: GABA
        Type: general
      – SubjectFull: Neurotransmitter receptors
        Type: general
      – SubjectFull: Chilo suppressalis
        Type: general
      – SubjectFull: Gene expression
        Type: general
    Titles:
      – TitleFull: Knockdown of the GABA receptor RDL genes decreases abamectin susceptibility in the rice stem borer, Chilo suppressalis.
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            NameFull: Meng, Xiangkun
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            NameFull: Yang, Xuemei
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            NameFull: Zhang, Nan
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            NameFull: Jiang, Heng
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            NameFull: Ge, Huichen
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            NameFull: Chen, Minxuan
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            – D: 01
              M: 01
              Text: Jan2019
              Type: published
              Y: 2019
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              Value: 153
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            – TitleFull: Pesticide Biochemistry & Physiology
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