View in EDS

Molecular characterization of glutamate-gated chloride channel and its possible roles in development and abamectin susceptibility in the rice stem borer, Chilo suppressalis.

Saved in:
Bibliographic Details
Title: Molecular characterization of glutamate-gated chloride channel and its possible roles in development and abamectin susceptibility in the rice stem borer, Chilo suppressalis.
Authors: Meng, Xiangkun1 (AUTHOR), Miao, Lijun1 (AUTHOR), Ge, Huichen1 (AUTHOR), Yang, Xuemei1 (AUTHOR), Dong, Fan1 (AUTHOR), Xu, Xin1 (AUTHOR), Wu, Zhaolu1 (AUTHOR), Qian, Kun1 (AUTHOR), Wang, Jianjun1 (AUTHOR) wangjj@yzu.edu.cn
Source: Pesticide Biochemistry & Physiology. Mar2019, Vol. 155, p72-80. 9p.
Subject Terms: Antisense DNA, Chilo suppressalis, Chloride channels
Abstract: Abstract Glutamate-gated chloride channels (GluCls) mediate fast inhibitory neurotransmission in invertebrate nervous systems, and are of considerable interest in insecticide discovery. The full length cDNA encoding CsGluCl was cloned from the rice stem borer Chilo suppressalis (Walker). Multiple cDNA sequence alignment revealed three variants of CsGluCl generated by alternative splicing of exon 3 and exon 9. While all the transcripts were predominantly expressed in both nerve cord and brain, the expression patterns of these three variants differed among other tissues and developmental stages. Specifically, the expression level of CsGluCl C in cuticle was similar to that in nerve cord and brain, and was the predominant variant in late pupae and early adult stages. Both injection and oral delivery of dsGluCl significantly reduced the mRNA level of CsGluCl. Increased susceptibility to abamectin and reduced larvae growth and pupation rate were observed in dsGluCl-treated larvae. Thus, our results provide the evidence that in addition to act as the target of abamectin, GluCls also play important physiological roles in the development of insects. Graphical abstract Unlabelled Image Highlights • Three variants of CsGluCl were generated by alternative splicing of exon 3. • CsGluCl C showed high expression in cuticle and was the predominant variant in late pupae and early adult stages. • Increased susceptibility to abamectin and reduced larvae growth and pupation rate were observed in dsGluCl -treated larvae. [ABSTRACT FROM AUTHOR]
Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: GreenFILE
Description
Abstract:Abstract Glutamate-gated chloride channels (GluCls) mediate fast inhibitory neurotransmission in invertebrate nervous systems, and are of considerable interest in insecticide discovery. The full length cDNA encoding CsGluCl was cloned from the rice stem borer Chilo suppressalis (Walker). Multiple cDNA sequence alignment revealed three variants of CsGluCl generated by alternative splicing of exon 3 and exon 9. While all the transcripts were predominantly expressed in both nerve cord and brain, the expression patterns of these three variants differed among other tissues and developmental stages. Specifically, the expression level of CsGluCl C in cuticle was similar to that in nerve cord and brain, and was the predominant variant in late pupae and early adult stages. Both injection and oral delivery of dsGluCl significantly reduced the mRNA level of CsGluCl. Increased susceptibility to abamectin and reduced larvae growth and pupation rate were observed in dsGluCl-treated larvae. Thus, our results provide the evidence that in addition to act as the target of abamectin, GluCls also play important physiological roles in the development of insects. Graphical abstract Unlabelled Image Highlights • Three variants of CsGluCl were generated by alternative splicing of exon 3. • CsGluCl C showed high expression in cuticle and was the predominant variant in late pupae and early adult stages. • Increased susceptibility to abamectin and reduced larvae growth and pupation rate were observed in dsGluCl -treated larvae. [ABSTRACT FROM AUTHOR]
ISSN:00483575
DOI:10.1016/j.pestbp.2019.01.007