View in EDS

Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review.

Saved in:
Bibliographic Details
Title: Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review.
Authors: Shanmugam, Sabarathinam1,2 (AUTHOR) sabashan87@stu.edu.cn, Ngo, Huu-Hao3 (AUTHOR) huuhao.ngo@uts.edu.au, Wu, Yi-Rui1,2,3 (AUTHOR) wuyr@stu.edu.cn
Source: Renewable Energy: An International Journal. Apr2020, Vol. 149, p1107-1119. 13p.
Subject Terms: *Bioengineering, *Molecular biology, *Biomass energy, *Butanol, *Ethanol as fuel, Genome editing, Zinc-finger proteins, Microbial genomes
Abstract: With rapid progress in the fields of synthetic biology and metabolic engineering, there are possible applications to generate a wide range of advanced biofuels with maximized yield and productivity to achieve a more sustainable bioprocess with reduced carbon footprints. Among the diverse molecular biology tools, clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) technology stands out with potential targeted genome editing, exhibiting a more precise and accurate gene knock-out and knock-in system better than its predecessors, for example zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN). There are reports involved in the advanced microbial genome engineering tools for the biofuels production; however, there is lack of a comprehensive review about the CRISPR-Cas based-techniques in improved biofuel production along with the strategies to reduce the off-target effect that ensures the success and safety of this method. Therefore, in this review we attempt to systematically comment on the mechanism of CRISPR-Cas and its application to microbial biofuels production. This includes bioethanol, biobutanol as well as other hydrocarbons that sequentially follow various suggestions on enhancing the efficiency of targeting genes. The role of inducible on/off genetic circuits in response to environmental stimuli in the regulation of targeted genome editing (TGE) by minimizing metabolic burden and maximizing fermentation efficiency is also discussed. The relevant stringent regulatory demands to ensure minimal off-target cleavage with maximum efficiency coupled with complete biosafety of this technology are considered. It can be concluded that the recent development of CRISPR-Cas technology should open a new avenue in creating microbial biorefineries for potentially enhanced biofuel production. • This review focuses on CRISPR-Cas technology in improved biofuels production. • Utilization of diverse CRISPR-Cas machinery for biofuels production was reviewed. • Enhancing the targeting gene efficiency by CRISPR genetic circuits were discussed. • Perspectives of methods for reducing off-target effects were summarized. [ABSTRACT FROM AUTHOR]
Copyright of Renewable Energy: An International Journal is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: GreenFILE
FullText Text:
  Availability: 0
Header DbId: 8gh
DbLabel: GreenFILE
An: 141737547
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Shanmugam%2C+Sabarathinam%22">Shanmugam, Sabarathinam</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> sabashan87@stu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Ngo%2C+Huu-Hao%22">Ngo, Huu-Hao</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> huuhao.ngo@uts.edu.au</i><br /><searchLink fieldCode="AR" term="%22Wu%2C+Yi-Rui%22">Wu, Yi-Rui</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> wuyr@stu.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Renewable+Energy%3A+An+International+Journal%22">Renewable Energy: An International Journal</searchLink>. Apr2020, Vol. 149, p1107-1119. 13p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Bioengineering%22">Bioengineering</searchLink><br />*<searchLink fieldCode="DE" term="%22Molecular+biology%22">Molecular biology</searchLink><br />*<searchLink fieldCode="DE" term="%22Biomass+energy%22">Biomass energy</searchLink><br />*<searchLink fieldCode="DE" term="%22Butanol%22">Butanol</searchLink><br />*<searchLink fieldCode="DE" term="%22Ethanol+as+fuel%22">Ethanol as fuel</searchLink><br /><searchLink fieldCode="DE" term="%22Genome+editing%22">Genome editing</searchLink><br /><searchLink fieldCode="DE" term="%22Zinc-finger+proteins%22">Zinc-finger proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+genomes%22">Microbial genomes</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: With rapid progress in the fields of synthetic biology and metabolic engineering, there are possible applications to generate a wide range of advanced biofuels with maximized yield and productivity to achieve a more sustainable bioprocess with reduced carbon footprints. Among the diverse molecular biology tools, clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) technology stands out with potential targeted genome editing, exhibiting a more precise and accurate gene knock-out and knock-in system better than its predecessors, for example zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN). There are reports involved in the advanced microbial genome engineering tools for the biofuels production; however, there is lack of a comprehensive review about the CRISPR-Cas based-techniques in improved biofuel production along with the strategies to reduce the off-target effect that ensures the success and safety of this method. Therefore, in this review we attempt to systematically comment on the mechanism of CRISPR-Cas and its application to microbial biofuels production. This includes bioethanol, biobutanol as well as other hydrocarbons that sequentially follow various suggestions on enhancing the efficiency of targeting genes. The role of inducible on/off genetic circuits in response to environmental stimuli in the regulation of targeted genome editing (TGE) by minimizing metabolic burden and maximizing fermentation efficiency is also discussed. The relevant stringent regulatory demands to ensure minimal off-target cleavage with maximum efficiency coupled with complete biosafety of this technology are considered. It can be concluded that the recent development of CRISPR-Cas technology should open a new avenue in creating microbial biorefineries for potentially enhanced biofuel production. • This review focuses on CRISPR-Cas technology in improved biofuels production. • Utilization of diverse CRISPR-Cas machinery for biofuels production was reviewed. • Enhancing the targeting gene efficiency by CRISPR genetic circuits were discussed. • Perspectives of methods for reducing off-target effects were summarized. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Renewable Energy: An International Journal is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=8gh&AN=141737547
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.renene.2019.10.107
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 13
        StartPage: 1107
    Subjects:
      – SubjectFull: Bioengineering
        Type: general
      – SubjectFull: Molecular biology
        Type: general
      – SubjectFull: Biomass energy
        Type: general
      – SubjectFull: Butanol
        Type: general
      – SubjectFull: Ethanol as fuel
        Type: general
      – SubjectFull: Genome editing
        Type: general
      – SubjectFull: Zinc-finger proteins
        Type: general
      – SubjectFull: Microbial genomes
        Type: general
    Titles:
      – TitleFull: Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Shanmugam, Sabarathinam
      – PersonEntity:
          Name:
            NameFull: Ngo, Huu-Hao
      – PersonEntity:
          Name:
            NameFull: Wu, Yi-Rui
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 15
              M: 04
              Text: Apr2020
              Type: published
              Y: 2020
          Identifiers:
            – Type: issn-print
              Value: 09601481
          Numbering:
            – Type: volume
              Value: 149
          Titles:
            – TitleFull: Renewable Energy: An International Journal
              Type: main
ResultId 1