Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna.
Saved in:
| Title: | Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna. |
|---|---|
| Authors: | Över, Sevgi Başalan1 (AUTHOR), Güven, Celal2 (AUTHOR), Taskin, Eylem3 (AUTHOR), Sevgiler, Yusuf4 (AUTHOR) ysevgiler@adiyaman.edu.tr |
| Source: | Archives of Industrial Hygiene & Toxicology / Arhiv za Higijenu Rada I Toksikologiju. Sep2020, Vol. 71 Issue 3, p211-222. 12p. |
| Subject Terms: | Daphnia magna, Serotonin uptake inhibitors, Mitochondrial membranes, Glutathione peroxidase, Membrane potential, Cytochrome c, Fluoxetine |
| Geographic Terms: | Türkiye |
| Abstract: | The aim of this study was to investigate the oxidative and apoptotic potential of fluoxetine, a widely used antidepressant in Turkey and the world, and of its metabolite norfluoxetine on a model non-target organism, Daphnia magna to see how exposure to this group of antidepressants (specific serotonin reuptake inhibitors) could affect the aquatic environment in which they end up. Juvenile D. magna specimens were chronically exposed to fluoxetine and norfluoxetine alone and in combination at concentrations found in the aquatic environment (0.091 and 0.011 μg/L, respectively) and to their 10-fold environmental concentrations for 21 days. Another group of 17-day-old animals were subacutely exposed to 100-fold environmental concentrations for four days. After exposure, we measured their glutathione peroxidase (GPx) and cholinesterase (ChE) activities, thiobarbituric acid-reactive substances (TBARS), and total protein content spectrophotometrically, while mitochondrial membrane potential (MMP) was analysed by fluorescence staining, and cytochrome c and ERK1/2 protein content by Western blotting. This is the first-time cytochrome c and ERK1/2 were determined at the protein level in D. magna. We also measured their carapace length, width, and caudal spine length microscopically. At environmental concentrations fluoxetine and norfluoxetine caused an increase in ChE activity and brood production. They also caused a decrease in juvenile carapace length, width, and caudal spine length and depolarised the mitochondrial membrane. At 10-fold environmental concentrations, GPx activity, lipid peroxidation levels, cytochrome c, and ERK1/2 protein levels rose. The most pronounced effect was observed in D. magna exposed to norfluoxetine. Norfluoxetine also decreased brood production. Similar effects were observed with subacute exposure to 100-fold environmental concentrations. However, total protein content decreased. All this confirms that fluoxetine and norfluoxetine have oxidative and apoptotic potential in D. magna. Daphnia spp. have a great potential to give us precious insight into the mechanisms of environmental toxicants, but there is still a long way to go before they are clarified in these organisms. [ABSTRACT FROM AUTHOR] |
| Copyright of Archives of Industrial Hygiene & Toxicology / Arhiv za Higijenu Rada I Toksikologiju is the property of Paradigm Publishing Services and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | GreenFILE |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: 8gh DbLabel: GreenFILE An: 146429989 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Över%2C+Sevgi+Başalan%22">Över, Sevgi Başalan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Güven%2C+Celal%22">Güven, Celal</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Taskin%2C+Eylem%22">Taskin, Eylem</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sevgiler%2C+Yusuf%22">Sevgiler, Yusuf</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> ysevgiler@adiyaman.edu.tr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Archives+of+Industrial+Hygiene+%26+Toxicology+%2F+Arhiv+za+Higijenu+Rada+I+Toksikologiju%22">Archives of Industrial Hygiene & Toxicology / Arhiv za Higijenu Rada I Toksikologiju</searchLink>. Sep2020, Vol. 71 Issue 3, p211-222. 12p. – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Daphnia+magna%22">Daphnia magna</searchLink><br /><searchLink fieldCode="DE" term="%22Serotonin+uptake+inhibitors%22">Serotonin uptake inhibitors</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondrial+membranes%22">Mitochondrial membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Glutathione+peroxidase%22">Glutathione peroxidase</searchLink><br /><searchLink fieldCode="DE" term="%22Membrane+potential%22">Membrane potential</searchLink><br /><searchLink fieldCode="DE" term="%22Cytochrome+c%22">Cytochrome c</searchLink><br /><searchLink fieldCode="DE" term="%22Fluoxetine%22">Fluoxetine</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Türkiye%22">Türkiye</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The aim of this study was to investigate the oxidative and apoptotic potential of fluoxetine, a widely used antidepressant in Turkey and the world, and of its metabolite norfluoxetine on a model non-target organism, Daphnia magna to see how exposure to this group of antidepressants (specific serotonin reuptake inhibitors) could affect the aquatic environment in which they end up. Juvenile D. magna specimens were chronically exposed to fluoxetine and norfluoxetine alone and in combination at concentrations found in the aquatic environment (0.091 and 0.011 μg/L, respectively) and to their 10-fold environmental concentrations for 21 days. Another group of 17-day-old animals were subacutely exposed to 100-fold environmental concentrations for four days. After exposure, we measured their glutathione peroxidase (GPx) and cholinesterase (ChE) activities, thiobarbituric acid-reactive substances (TBARS), and total protein content spectrophotometrically, while mitochondrial membrane potential (MMP) was analysed by fluorescence staining, and cytochrome c and ERK1/2 protein content by Western blotting. This is the first-time cytochrome c and ERK1/2 were determined at the protein level in D. magna. We also measured their carapace length, width, and caudal spine length microscopically. At environmental concentrations fluoxetine and norfluoxetine caused an increase in ChE activity and brood production. They also caused a decrease in juvenile carapace length, width, and caudal spine length and depolarised the mitochondrial membrane. At 10-fold environmental concentrations, GPx activity, lipid peroxidation levels, cytochrome c, and ERK1/2 protein levels rose. The most pronounced effect was observed in D. magna exposed to norfluoxetine. Norfluoxetine also decreased brood production. Similar effects were observed with subacute exposure to 100-fold environmental concentrations. However, total protein content decreased. All this confirms that fluoxetine and norfluoxetine have oxidative and apoptotic potential in D. magna. Daphnia spp. have a great potential to give us precious insight into the mechanisms of environmental toxicants, but there is still a long way to go before they are clarified in these organisms. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Archives of Industrial Hygiene & Toxicology / Arhiv za Higijenu Rada I Toksikologiju is the property of Paradigm Publishing Services and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=8gh&AN=146429989 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.2478/aiht-2020-71-3473 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 211 Subjects: – SubjectFull: Daphnia magna Type: general – SubjectFull: Serotonin uptake inhibitors Type: general – SubjectFull: Mitochondrial membranes Type: general – SubjectFull: Glutathione peroxidase Type: general – SubjectFull: Membrane potential Type: general – SubjectFull: Cytochrome c Type: general – SubjectFull: Fluoxetine Type: general – SubjectFull: Türkiye Type: general Titles: – TitleFull: Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Över, Sevgi Başalan – PersonEntity: Name: NameFull: Güven, Celal – PersonEntity: Name: NameFull: Taskin, Eylem – PersonEntity: Name: NameFull: Sevgiler, Yusuf IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 00041254 Numbering: – Type: volume Value: 71 – Type: issue Value: 3 Titles: – TitleFull: Archives of Industrial Hygiene & Toxicology / Arhiv za Higijenu Rada I Toksikologiju Type: main |
| ResultId | 1 |
