View in EDS PDF Full Text

Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis.

Saved in:
Bibliographic Details
Title: Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis.
Authors: Piiion, Arnaud1, Boussioux, Anne-Marie1, Escande, Aurélie1, Aït-Aïssa, Sélim2, Gomez, Elena3, Fenet, Héléne3, Ruff, Marc4, Moras, Dino4, Vignon, Françoise1, Duchesne, Marie-Joséphe1, Casellas, Claude3, Nicolas, Jean-Claude1, Balaguer, Patrick1 balaguer@montp.inserm.fr
Source: Environmental Health Perspectives. Mar2005, Vol. 113 Issue 3, p278-284. 7p.
Subject Terms: *Phthalate esters, *Polychlorinated biphenyls, *Polycyclic aromatic hydrocarbons, *River sediments, Estrogen receptors
Abstract: Estrogenic activity in environmental samples could be mediated through a wide variety of compounds and by various mechanisms. High-affinity compounds for estrogen receptors (ERs), such as natural or synthetic estrogens, as well as low-affinity compounds such as alkylphenols, phthalates, and polychlorinated biphenyls are present in water and sediment samples. Furthermore, compounds such as polycyclic aromatic hydrocarbons, which do not bind ERs, modulate estrogen activity by means of the aryl hydrocarbon receptor (AhR). In order to characterize compounds that mediate estrogenic activity in river water and sediment samples, we developed a tool based on the ER-α ligand-binding domain, which permitted us to estimate contaminating estrogenic compound affinities. We designed a simple transactivation assay in which compounds of high affinity were captured by limited amounts of recombinant ER-α and whose capture led to a selective inhibition of transactivation. This approach allowed us to bring to light that water samples contain estrogenic compounds that display a high affinity for ERs but are present at low concentrations. In sediment samples, on the contrary, we showed that estrogenic compounds possess a low affinity and are present at high concentration. Finally, we used immobilized recombinant ER-α to separate ligands for ER and AhR that are present in river sediments. Immobilized ER-α, which does not retain dioxin-like compounds, enabled us to isolate and concentrate ER ligands to facilitate their further analysis. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Health Perspectives is the property of National Institute of Environmental Health Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: GreenFILE
FullText Links:
  – Type: pdflink
Text:
  Availability: 0
Header DbId: 8gh
DbLabel: GreenFILE
An: 16686763
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Piiion%2C+Arnaud%22">Piiion, Arnaud</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Boussioux%2C+Anne-Marie%22">Boussioux, Anne-Marie</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Escande%2C+Aurélie%22">Escande, Aurélie</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Aït-Aïssa%2C+Sélim%22">Aït-Aïssa, Sélim</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Gomez%2C+Elena%22">Gomez, Elena</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Fenet%2C+Héléne%22">Fenet, Héléne</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ruff%2C+Marc%22">Ruff, Marc</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Moras%2C+Dino%22">Moras, Dino</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Vignon%2C+Françoise%22">Vignon, Françoise</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Duchesne%2C+Marie-Joséphe%22">Duchesne, Marie-Joséphe</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Casellas%2C+Claude%22">Casellas, Claude</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Nicolas%2C+Jean-Claude%22">Nicolas, Jean-Claude</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Balaguer%2C+Patrick%22">Balaguer, Patrick</searchLink><relatesTo>1</relatesTo><i> balaguer@montp.inserm.fr</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Environmental+Health+Perspectives%22">Environmental Health Perspectives</searchLink>. Mar2005, Vol. 113 Issue 3, p278-284. 7p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Phthalate+esters%22">Phthalate esters</searchLink><br />*<searchLink fieldCode="DE" term="%22Polychlorinated+biphenyls%22">Polychlorinated biphenyls</searchLink><br />*<searchLink fieldCode="DE" term="%22Polycyclic+aromatic+hydrocarbons%22">Polycyclic aromatic hydrocarbons</searchLink><br />*<searchLink fieldCode="DE" term="%22River+sediments%22">River sediments</searchLink><br /><searchLink fieldCode="DE" term="%22Estrogen+receptors%22">Estrogen receptors</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Estrogenic activity in environmental samples could be mediated through a wide variety of compounds and by various mechanisms. High-affinity compounds for estrogen receptors (ERs), such as natural or synthetic estrogens, as well as low-affinity compounds such as alkylphenols, phthalates, and polychlorinated biphenyls are present in water and sediment samples. Furthermore, compounds such as polycyclic aromatic hydrocarbons, which do not bind ERs, modulate estrogen activity by means of the aryl hydrocarbon receptor (AhR). In order to characterize compounds that mediate estrogenic activity in river water and sediment samples, we developed a tool based on the ER-α ligand-binding domain, which permitted us to estimate contaminating estrogenic compound affinities. We designed a simple transactivation assay in which compounds of high affinity were captured by limited amounts of recombinant ER-α and whose capture led to a selective inhibition of transactivation. This approach allowed us to bring to light that water samples contain estrogenic compounds that display a high affinity for ERs but are present at low concentrations. In sediment samples, on the contrary, we showed that estrogenic compounds possess a low affinity and are present at high concentration. Finally, we used immobilized recombinant ER-α to separate ligands for ER and AhR that are present in river sediments. Immobilized ER-α, which does not retain dioxin-like compounds, enabled us to isolate and concentrate ER ligands to facilitate their further analysis. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Environmental Health Perspectives is the property of National Institute of Environmental Health Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=8gh&AN=16686763
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1289/ehp.7522
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 278
    Subjects:
      – SubjectFull: Phthalate esters
        Type: general
      – SubjectFull: Polychlorinated biphenyls
        Type: general
      – SubjectFull: Polycyclic aromatic hydrocarbons
        Type: general
      – SubjectFull: River sediments
        Type: general
      – SubjectFull: Estrogen receptors
        Type: general
    Titles:
      – TitleFull: Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Piiion, Arnaud
      – PersonEntity:
          Name:
            NameFull: Boussioux, Anne-Marie
      – PersonEntity:
          Name:
            NameFull: Escande, Aurélie
      – PersonEntity:
          Name:
            NameFull: Aït-Aïssa, Sélim
      – PersonEntity:
          Name:
            NameFull: Gomez, Elena
      – PersonEntity:
          Name:
            NameFull: Fenet, Héléne
      – PersonEntity:
          Name:
            NameFull: Ruff, Marc
      – PersonEntity:
          Name:
            NameFull: Moras, Dino
      – PersonEntity:
          Name:
            NameFull: Vignon, Françoise
      – PersonEntity:
          Name:
            NameFull: Duchesne, Marie-Joséphe
      – PersonEntity:
          Name:
            NameFull: Casellas, Claude
      – PersonEntity:
          Name:
            NameFull: Nicolas, Jean-Claude
      – PersonEntity:
          Name:
            NameFull: Balaguer, Patrick
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 03
              Text: Mar2005
              Type: published
              Y: 2005
          Identifiers:
            – Type: issn-print
              Value: 00916765
          Numbering:
            – Type: volume
              Value: 113
            – Type: issue
              Value: 3
          Titles:
            – TitleFull: Environmental Health Perspectives
              Type: main
ResultId 1