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Evaluation of the cytotoxicity and genotoxicity of glufosinate-ammonium at technical and commercial grades in HepG2 cells.

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Title: Evaluation of the cytotoxicity and genotoxicity of glufosinate-ammonium at technical and commercial grades in HepG2 cells.
Authors: Gallardo-Valle, Ezeidy Denisse1 (AUTHOR), Carbajal-Nogueda, Dayanne1 (AUTHOR), Moreno-Godínez, Ma. Elena1 (AUTHOR), Flores-Alfaro, Eugenia2 (AUTHOR), Parra-Rojas, Isela3 (AUTHOR), Huerta-Beristain, Gerardo1 (AUTHOR), Domínguez-Reyes, Teresa3 (AUTHOR), Ramírez-Vargas, Marco Antonio1 (AUTHOR)
Source: Journal of Environmental Science & Health. Part B. Pesticides, Food Contaminants & Agricultural Wastes. 2023, Vol. 58 Issue 8, p577-582. 6p.
Subject Terms: *Genetic toxicology, *Carcinogenesis, *Pesticides, Cell survival, Technical reports, DNA damage, Comets
Abstract: Exposure to genotoxic agents is associated with the development of cancer and related diseases. For this reason, assessing the genotoxicity of chemical compounds is necessary. In this line, information about the genotoxic effect of glufosinate-ammonium (GLA) has been reported only for the technical grade. However, humans are frequently exposed to commercial formulations of pesticides. Commercial formulations are characterized by using inner agents that increase toxicity compared to pesticides in technical grade. This study aimed to determine the cytotoxic and genotoxic effects of GLA on HepG2 cells. MTT and comet assays were performed to evaluate cell viability and DNA damage, respectively. HepG2 cells were exposed for 24 h to different concentrations of GLA (at 0.01 µg/mL; 0.04 µg/mL; 0.1 µg/mL; 0.24 µg/mL; 0.52 µg/mL; 1.25 µg/mL; 2.62 µg/mL and 13.12 µg/mL) in commercial- (Finale Ultra®) or technical-grade (GLAT). The results indicated that only Finale Ultra® induced a reduction in cell viability at 13.12 µg/mL. Furthermore, exposure to Finale Ultra® or GLAT was associated with increased DNA damage at concentrations from 0.52–13.12- µg/mL. This study shows the genotoxic effect of GLA on HepG2 cells. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Environmental Science & Health. Part B. Pesticides, Food Contaminants & Agricultural Wastes is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Evaluation of the cytotoxicity and genotoxicity of glufosinate-ammonium at technical and commercial grades in HepG2 cells.
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  Data: <searchLink fieldCode="AR" term="%22Gallardo-Valle%2C+Ezeidy+Denisse%22">Gallardo-Valle, Ezeidy Denisse</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Carbajal-Nogueda%2C+Dayanne%22">Carbajal-Nogueda, Dayanne</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Moreno-Godínez%2C+Ma%2E+Elena%22">Moreno-Godínez, Ma. Elena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Flores-Alfaro%2C+Eugenia%22">Flores-Alfaro, Eugenia</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Parra-Rojas%2C+Isela%22">Parra-Rojas, Isela</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huerta-Beristain%2C+Gerardo%22">Huerta-Beristain, Gerardo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Domínguez-Reyes%2C+Teresa%22">Domínguez-Reyes, Teresa</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ramírez-Vargas%2C+Marco+Antonio%22">Ramírez-Vargas, Marco Antonio</searchLink><relatesTo>1</relatesTo> (AUTHOR)
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  Data: *<searchLink fieldCode="DE" term="%22Genetic+toxicology%22">Genetic toxicology</searchLink><br />*<searchLink fieldCode="DE" term="%22Carcinogenesis%22">Carcinogenesis</searchLink><br />*<searchLink fieldCode="DE" term="%22Pesticides%22">Pesticides</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+survival%22">Cell survival</searchLink><br /><searchLink fieldCode="DE" term="%22Technical+reports%22">Technical reports</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22Comets%22">Comets</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Exposure to genotoxic agents is associated with the development of cancer and related diseases. For this reason, assessing the genotoxicity of chemical compounds is necessary. In this line, information about the genotoxic effect of glufosinate-ammonium (GLA) has been reported only for the technical grade. However, humans are frequently exposed to commercial formulations of pesticides. Commercial formulations are characterized by using inner agents that increase toxicity compared to pesticides in technical grade. This study aimed to determine the cytotoxic and genotoxic effects of GLA on HepG2 cells. MTT and comet assays were performed to evaluate cell viability and DNA damage, respectively. HepG2 cells were exposed for 24 h to different concentrations of GLA (at 0.01 µg/mL; 0.04 µg/mL; 0.1 µg/mL; 0.24 µg/mL; 0.52 µg/mL; 1.25 µg/mL; 2.62 µg/mL and 13.12 µg/mL) in commercial- (Finale Ultra®) or technical-grade (GLAT). The results indicated that only Finale Ultra® induced a reduction in cell viability at 13.12 µg/mL. Furthermore, exposure to Finale Ultra® or GLAT was associated with increased DNA damage at concentrations from 0.52–13.12- µg/mL. This study shows the genotoxic effect of GLA on HepG2 cells. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Environmental Science & Health. Part B. Pesticides, Food Contaminants & Agricultural Wastes is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1080/03601234.2023.2241322
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        Text: English
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      Pagination:
        PageCount: 6
        StartPage: 577
    Subjects:
      – SubjectFull: Genetic toxicology
        Type: general
      – SubjectFull: Carcinogenesis
        Type: general
      – SubjectFull: Pesticides
        Type: general
      – SubjectFull: Cell survival
        Type: general
      – SubjectFull: Technical reports
        Type: general
      – SubjectFull: DNA damage
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      – SubjectFull: Comets
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      – TitleFull: Evaluation of the cytotoxicity and genotoxicity of glufosinate-ammonium at technical and commercial grades in HepG2 cells.
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            NameFull: Carbajal-Nogueda, Dayanne
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            – D: 01
              M: 08
              Text: 2023
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