Aberrant splicing of a nicotinic acetylcholine receptor alpha 6 subunit is associated with spinosad tolerance in the thrips predator Orius laevigatus.
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| Title: | Aberrant splicing of a nicotinic acetylcholine receptor alpha 6 subunit is associated with spinosad tolerance in the thrips predator Orius laevigatus. |
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| Authors: | Zeng, Bin1 (AUTHOR), Hunt, Benjamin J.1 (AUTHOR), Pym, Adam1 (AUTHOR), Balanza, Virginia2 (AUTHOR), Bass, Chris1 (AUTHOR), Bielza, Pablo1,2 (AUTHOR), Troczka, Bartlomiej J.1 (AUTHOR) b.troczka@exeter.ac.uk |
| Source: | Pesticide Biochemistry & Physiology. Mar2024, Vol. 200, pN.PAG-N.PAG. 1p. |
| Subject Terms: | *Biological pest control agents, *Integrated pest control, *Insecticides, Nicotinic acetylcholine receptors, Spinosad, Cholinergic receptors, Conotoxins, Thrips |
| Abstract: | Susceptibility to insecticides is one of the limiting factors preventing wider adoption of natural enemies to control insect pest populations. Identification and selective breeding of insecticide tolerant strains of commercially used biological control agents (BCAs) is one of the approaches to overcome this constraint. Although a number of beneficial insects have been selected for increased tolerance to insecticides the molecular mechanisms underpinning these shifts in tolerance are not well characterised. Here we investigated the molecular mechanisms of enhanced tolerance of a lab selected strain of Orius laevigatus (Fieber) to the commonly used biopesticide spinosad. Transcriptomic analysis showed that spinosad tolerance is not a result of overexpressed detoxification genes. Molecular analysis of the target site for spinosyns, the nicotinic acetylcholine receptor (nAChR), revealed increased expression of truncated transcripts of the nAChR α6 subunit in the spinosad selected strain, a mechanism of resistance which was described previously in insect pest species. Collectively, our results demonstrate the mechanisms by which some beneficial biological control agents can evolve insecticide tolerance and will inform the development and deployment of insecticide-tolerant natural enemies in integrated pest management strategies. [Display omitted] • Spinosad resistance in Orius laevigatus is not driven by the upregulation of detoxification enzymes. • SNPs identified in the spinosad target site NaChR α6 subunit are unlikely to play a role in resistance. • An increase in expression of truncated α6 subunit transcripts identified in the resistant strain. [ABSTRACT FROM AUTHOR] |
| Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | GreenFILE |
| FullText | Text: Availability: 0 |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 176437125 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Aberrant splicing of a nicotinic acetylcholine receptor alpha 6 subunit is associated with spinosad tolerance in the thrips predator Orius laevigatus. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zeng%2C+Bin%22">Zeng, Bin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hunt%2C+Benjamin+J%2E%22">Hunt, Benjamin J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pym%2C+Adam%22">Pym, Adam</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Balanza%2C+Virginia%22">Balanza, Virginia</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bass%2C+Chris%22">Bass, Chris</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bielza%2C+Pablo%22">Bielza, Pablo</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Troczka%2C+Bartlomiej+J%2E%22">Troczka, Bartlomiej J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> b.troczka@exeter.ac.uk</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. Mar2024, Vol. 200, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22Biological+pest+control+agents%22">Biological pest control agents</searchLink><br />*<searchLink fieldCode="DE" term="%22Integrated+pest+control%22">Integrated pest control</searchLink><br />*<searchLink fieldCode="DE" term="%22Insecticides%22">Insecticides</searchLink><br /><searchLink fieldCode="DE" term="%22Nicotinic+acetylcholine+receptors%22">Nicotinic acetylcholine receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Spinosad%22">Spinosad</searchLink><br /><searchLink fieldCode="DE" term="%22Cholinergic+receptors%22">Cholinergic receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Conotoxins%22">Conotoxins</searchLink><br /><searchLink fieldCode="DE" term="%22Thrips%22">Thrips</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Susceptibility to insecticides is one of the limiting factors preventing wider adoption of natural enemies to control insect pest populations. Identification and selective breeding of insecticide tolerant strains of commercially used biological control agents (BCAs) is one of the approaches to overcome this constraint. Although a number of beneficial insects have been selected for increased tolerance to insecticides the molecular mechanisms underpinning these shifts in tolerance are not well characterised. Here we investigated the molecular mechanisms of enhanced tolerance of a lab selected strain of Orius laevigatus (Fieber) to the commonly used biopesticide spinosad. Transcriptomic analysis showed that spinosad tolerance is not a result of overexpressed detoxification genes. Molecular analysis of the target site for spinosyns, the nicotinic acetylcholine receptor (nAChR), revealed increased expression of truncated transcripts of the nAChR α6 subunit in the spinosad selected strain, a mechanism of resistance which was described previously in insect pest species. Collectively, our results demonstrate the mechanisms by which some beneficial biological control agents can evolve insecticide tolerance and will inform the development and deployment of insecticide-tolerant natural enemies in integrated pest management strategies. [Display omitted] • Spinosad resistance in Orius laevigatus is not driven by the upregulation of detoxification enzymes. • SNPs identified in the spinosad target site NaChR α6 subunit are unlikely to play a role in resistance. • An increase in expression of truncated α6 subunit transcripts identified in the resistant strain. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.pestbp.2024.105837 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Biological pest control agents Type: general – SubjectFull: Integrated pest control Type: general – SubjectFull: Insecticides Type: general – SubjectFull: Nicotinic acetylcholine receptors Type: general – SubjectFull: Spinosad Type: general – SubjectFull: Cholinergic receptors Type: general – SubjectFull: Conotoxins Type: general – SubjectFull: Thrips Type: general Titles: – TitleFull: Aberrant splicing of a nicotinic acetylcholine receptor alpha 6 subunit is associated with spinosad tolerance in the thrips predator Orius laevigatus. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zeng, Bin – PersonEntity: Name: NameFull: Hunt, Benjamin J. – PersonEntity: Name: NameFull: Pym, Adam – PersonEntity: Name: NameFull: Balanza, Virginia – PersonEntity: Name: NameFull: Bass, Chris – PersonEntity: Name: NameFull: Bielza, Pablo – PersonEntity: Name: NameFull: Troczka, Bartlomiej J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 00483575 Numbering: – Type: volume Value: 200 Titles: – TitleFull: Pesticide Biochemistry & Physiology Type: main |
| ResultId | 1 |
