Polycyclic aromatic hydrocarbons in human granulosa cells: first in vivo presence and positive correlation with body mass index and in vitro ovarian cell steroidogenesis regulation.
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| Title: | Polycyclic aromatic hydrocarbons in human granulosa cells: first in vivo presence and positive correlation with body mass index and in vitro ovarian cell steroidogenesis regulation. |
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| Authors: | Kurowska, Patrycja1 (AUTHOR) patrycja.kurowska@uj.edu.pl, Berthet, Lucille2 (AUTHOR) lucille.berthet@inrae.fr, Ramé, Christelle2 (AUTHOR) Christelle.rame@inrae.fr, Węgiel, Małgorzata3 (AUTHOR) malgorzata.wegiel@pk.edu.pl, Maślanka, Anna3 (AUTHOR) anna.maslanka@pk.edu.pl, Guérif, Fabrice4 (AUTHOR) fabrice.guerif@univ-tours.fr, Froment, Pascal2 (AUTHOR) pascal.froment@inrae.fr, Rak, Agnieszka1 (AUTHOR) agnieszka.rak@uj.edu.pl, Dupont, Joelle1,2 (AUTHOR) joelle.dupont@inrae.fr |
| Source: | Environmental Toxicology & Pharmacology. Jan2025, Vol. 113, pN.PAG-N.PAG. 1p. |
| Subject Terms: | *Polycyclic aromatic hydrocarbons, *Acenaphthene, *Phenanthrene, Granulosa cells, Fluoranthene, Body mass index |
| Abstract: | Polycyclic Aromatic Hydrocarbons (PAHs) exposure leads to disorders reported in female infertility patients. Our hypothesis is that PAHs accumulate in granulosa cells (Gc) according to body mass index (BMI) and directly affects its functions. All 16 high-priority PAHs were in human FF, Gc and blood plasma with the highest concentration in Gc (GC–MS/MS). Their highest concentration was in obese Gc, except for acenaphthene and acenaphthylene, and positively correlated with BMI. In FF, we noted only positive correlation between naphthalene and BMI, whereas in blood plasma positive correlation between naphthalene, acenaphthene, pyrene and BMI. Phenanthrene and naphthalene but not fluoranthene inhibited totally steroidogenesis (ELISA), CYP19A1 mRNA expression (real-time PCR) and increased oxidative stress index and catalase expression in Gc independently on BMI. While all studied PAHs decreased Gc proliferation (BrdU assay) and viability (Cell Count kit-8 assay). Thus, Gc PAHs concentrations are positively correlated with BMI and alter ovarian functions. • PAHs in human granulosa cells are positively correlated with Body Mass Index. • Phenanthrene and naphthalene totally reduced human granulosa cells steroidogenesis. • Phenanthrene, naphthalene but not fluoranthene increased oxidative stress. • Phenanthrene naphthalene and fluoranthene reduced cell proliferation and viability. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Toxicology & Pharmacology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 182095516 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Polycyclic aromatic hydrocarbons in human granulosa cells: first in vivo presence and positive correlation with body mass index and in vitro ovarian cell steroidogenesis regulation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kurowska%2C+Patrycja%22">Kurowska, Patrycja</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> patrycja.kurowska@uj.edu.pl</i><br /><searchLink fieldCode="AR" term="%22Berthet%2C+Lucille%22">Berthet, Lucille</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> lucille.berthet@inrae.fr</i><br /><searchLink fieldCode="AR" term="%22Ramé%2C+Christelle%22">Ramé, Christelle</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> Christelle.rame@inrae.fr</i><br /><searchLink fieldCode="AR" term="%22Węgiel%2C+Małgorzata%22">Węgiel, Małgorzata</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> malgorzata.wegiel@pk.edu.pl</i><br /><searchLink fieldCode="AR" term="%22Maślanka%2C+Anna%22">Maślanka, Anna</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> anna.maslanka@pk.edu.pl</i><br /><searchLink fieldCode="AR" term="%22Guérif%2C+Fabrice%22">Guérif, Fabrice</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> fabrice.guerif@univ-tours.fr</i><br /><searchLink fieldCode="AR" term="%22Froment%2C+Pascal%22">Froment, Pascal</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> pascal.froment@inrae.fr</i><br /><searchLink fieldCode="AR" term="%22Rak%2C+Agnieszka%22">Rak, Agnieszka</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> agnieszka.rak@uj.edu.pl</i><br /><searchLink fieldCode="AR" term="%22Dupont%2C+Joelle%22">Dupont, Joelle</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> joelle.dupont@inrae.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Toxicology+%26+Pharmacology%22">Environmental Toxicology & Pharmacology</searchLink>. Jan2025, Vol. 113, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22Polycyclic+aromatic+hydrocarbons%22">Polycyclic aromatic hydrocarbons</searchLink><br />*<searchLink fieldCode="DE" term="%22Acenaphthene%22">Acenaphthene</searchLink><br />*<searchLink fieldCode="DE" term="%22Phenanthrene%22">Phenanthrene</searchLink><br /><searchLink fieldCode="DE" term="%22Granulosa+cells%22">Granulosa cells</searchLink><br /><searchLink fieldCode="DE" term="%22Fluoranthene%22">Fluoranthene</searchLink><br /><searchLink fieldCode="DE" term="%22Body+mass+index%22">Body mass index</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Polycyclic Aromatic Hydrocarbons (PAHs) exposure leads to disorders reported in female infertility patients. Our hypothesis is that PAHs accumulate in granulosa cells (Gc) according to body mass index (BMI) and directly affects its functions. All 16 high-priority PAHs were in human FF, Gc and blood plasma with the highest concentration in Gc (GC–MS/MS). Their highest concentration was in obese Gc, except for acenaphthene and acenaphthylene, and positively correlated with BMI. In FF, we noted only positive correlation between naphthalene and BMI, whereas in blood plasma positive correlation between naphthalene, acenaphthene, pyrene and BMI. Phenanthrene and naphthalene but not fluoranthene inhibited totally steroidogenesis (ELISA), CYP19A1 mRNA expression (real-time PCR) and increased oxidative stress index and catalase expression in Gc independently on BMI. While all studied PAHs decreased Gc proliferation (BrdU assay) and viability (Cell Count kit-8 assay). Thus, Gc PAHs concentrations are positively correlated with BMI and alter ovarian functions. • PAHs in human granulosa cells are positively correlated with Body Mass Index. • Phenanthrene and naphthalene totally reduced human granulosa cells steroidogenesis. • Phenanthrene, naphthalene but not fluoranthene increased oxidative stress. • Phenanthrene naphthalene and fluoranthene reduced cell proliferation and viability. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Toxicology & Pharmacology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.etap.2024.104611 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Polycyclic aromatic hydrocarbons Type: general – SubjectFull: Acenaphthene Type: general – SubjectFull: Phenanthrene Type: general – SubjectFull: Granulosa cells Type: general – SubjectFull: Fluoranthene Type: general – SubjectFull: Body mass index Type: general Titles: – TitleFull: Polycyclic aromatic hydrocarbons in human granulosa cells: first in vivo presence and positive correlation with body mass index and in vitro ovarian cell steroidogenesis regulation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kurowska, Patrycja – PersonEntity: Name: NameFull: Berthet, Lucille – PersonEntity: Name: NameFull: Ramé, Christelle – PersonEntity: Name: NameFull: Węgiel, Małgorzata – PersonEntity: Name: NameFull: Maślanka, Anna – PersonEntity: Name: NameFull: Guérif, Fabrice – PersonEntity: Name: NameFull: Froment, Pascal – PersonEntity: Name: NameFull: Rak, Agnieszka – PersonEntity: Name: NameFull: Dupont, Joelle IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 13826689 Numbering: – Type: volume Value: 113 Titles: – TitleFull: Environmental Toxicology & Pharmacology Type: main |
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