Exploring the Zein/58S Bioactive Glass Nanocomposite for Enhanced Bone Tissue Engineering: A Comprehensive Investigation of Structural, Chemical, Biological, and Osteogenic Properties through in Vitro and in Vivo Studies.
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| Title: | Exploring the Zein/58S Bioactive Glass Nanocomposite for Enhanced Bone Tissue Engineering: A Comprehensive Investigation of Structural, Chemical, Biological, and Osteogenic Properties through in Vitro and in Vivo Studies. |
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| Authors: | Esmaeili Ranjbar, Faezeh1 (AUTHOR), Mohandesnezhad, Sanam2 (AUTHOR), Mirzaei-Parsa, Mohamad Javad3,4 (AUTHOR), Asadi, Fatemeh1 (AUTHOR), Divanpour, Samalireza5 (AUTHOR), Karimabad, Mojgan Noroozi1 (AUTHOR), Vatanparast, Mahboubeh1 (AUTHOR), Mirzaei, Mohammad Reza1 (AUTHOR), Hassanshahi, Gholamhossein1 (AUTHOR), Tayebi, Lobat6,7 (AUTHOR) Ltayebi@odu.edu, Esmaeili Ranjbar, Afsaneh8 (AUTHOR) esmaeiliranjbar.afsaneh@gmail.com |
| Source: | Journal of Polymers & the Environment. Jan2025, Vol. 33 Issue 1, p462-482. 21p. |
| Subject Terms: | Young's modulus, Biomedical engineering, Cell adhesion, Tissue engineering, Contact angle, Bioactive glasses, Zein (Plant protein) |
| Abstract: | Bone tissue engineering has emerged as an innovative approach for repairing and regenerating bone defects. This study focuses on the development of new scaffolds with key attributes, including biocompatibility, bioactivity, biodegradability, cost effectiveness, and safety. In this investigation, we designed and synthesized a novel nanofibrous scaffold using the electrospinning method, incorporating zein/58S bioactive glass. The manufactured scaffolds underwent comprehensive characterization for morphology, sustainability, and chemical structure. Moreover, to demonstrate their efficacy in bone healing, we quantified essential factors such as biodegradation rate, contact angle, mechanical strength, bioactivity, cytotoxicity, and cell adherence. Following that, the osteogenesis effect of scaffolds was evaluated in vitro as well as in vivo through implanting them in the calvarium of the rats. Specifically, we conducted detailed investigations using alizarin red staining, real-time PCR, and histopathology, along with immunohistochemistry assessments. Based on our results, the fiber diameters were about 160.2 ± 7 nm, 163.5 ± 38.3 nm, and 164 ± 39.3 nm, respectively for zein, 2%BG, and 4%BG mats. Incorporation of 58 S increased contact angle from 96.03 ± 0.7° to 51.7 ± 2.02°, and consequently improved cell adhesion. The degradation rate of all scaffolds was about 20%, and chemical analysis (FTIR) confirmed the presence of 58 S in zein nanoscale mats. Tensile analysis presented that applying bioactive glass rescued Young's modulus from 0.34 ± 0.07 to 0.08 ± 0.009 MPa. Meanwhile, other results revealed that 4%BG scaffolds exhibit desirable properties, being porous, safe, bioactive, and osteogenic. These findings robustly affirm the competence and potential of the manufactured nanofibrous scaffold containing 4%BG for applications in bone tissue engineering. The schematic diagram illustrating different stages of the study, including; zein/BG scaffold synthesis, characterizations and osteogenesis evaluation in vitro and in vivo [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Polymers & the Environment is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 182191001 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Exploring the Zein/58S Bioactive Glass Nanocomposite for Enhanced Bone Tissue Engineering: A Comprehensive Investigation of Structural, Chemical, Biological, and Osteogenic Properties through in Vitro and in Vivo Studies. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Esmaeili+Ranjbar%2C+Faezeh%22">Esmaeili Ranjbar, Faezeh</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mohandesnezhad%2C+Sanam%22">Mohandesnezhad, Sanam</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mirzaei-Parsa%2C+Mohamad+Javad%22">Mirzaei-Parsa, Mohamad Javad</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Asadi%2C+Fatemeh%22">Asadi, Fatemeh</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Divanpour%2C+Samalireza%22">Divanpour, Samalireza</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Karimabad%2C+Mojgan+Noroozi%22">Karimabad, Mojgan Noroozi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vatanparast%2C+Mahboubeh%22">Vatanparast, Mahboubeh</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mirzaei%2C+Mohammad+Reza%22">Mirzaei, Mohammad Reza</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hassanshahi%2C+Gholamhossein%22">Hassanshahi, Gholamhossein</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tayebi%2C+Lobat%22">Tayebi, Lobat</searchLink><relatesTo>6,7</relatesTo> (AUTHOR)<i> Ltayebi@odu.edu</i><br /><searchLink fieldCode="AR" term="%22Esmaeili+Ranjbar%2C+Afsaneh%22">Esmaeili Ranjbar, Afsaneh</searchLink><relatesTo>8</relatesTo> (AUTHOR)<i> esmaeiliranjbar.afsaneh@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Polymers+%26+the+Environment%22">Journal of Polymers & the Environment</searchLink>. Jan2025, Vol. 33 Issue 1, p462-482. 21p. – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Young's+modulus%22">Young's modulus</searchLink><br /><searchLink fieldCode="DE" term="%22Biomedical+engineering%22">Biomedical engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+adhesion%22">Cell adhesion</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+engineering%22">Tissue engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Contact+angle%22">Contact angle</searchLink><br /><searchLink fieldCode="DE" term="%22Bioactive+glasses%22">Bioactive glasses</searchLink><br /><searchLink fieldCode="DE" term="%22Zein+%28Plant+protein%29%22">Zein (Plant protein)</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Bone tissue engineering has emerged as an innovative approach for repairing and regenerating bone defects. This study focuses on the development of new scaffolds with key attributes, including biocompatibility, bioactivity, biodegradability, cost effectiveness, and safety. In this investigation, we designed and synthesized a novel nanofibrous scaffold using the electrospinning method, incorporating zein/58S bioactive glass. The manufactured scaffolds underwent comprehensive characterization for morphology, sustainability, and chemical structure. Moreover, to demonstrate their efficacy in bone healing, we quantified essential factors such as biodegradation rate, contact angle, mechanical strength, bioactivity, cytotoxicity, and cell adherence. Following that, the osteogenesis effect of scaffolds was evaluated in vitro as well as in vivo through implanting them in the calvarium of the rats. Specifically, we conducted detailed investigations using alizarin red staining, real-time PCR, and histopathology, along with immunohistochemistry assessments. Based on our results, the fiber diameters were about 160.2 ± 7 nm, 163.5 ± 38.3 nm, and 164 ± 39.3 nm, respectively for zein, 2%BG, and 4%BG mats. Incorporation of 58 S increased contact angle from 96.03 ± 0.7° to 51.7 ± 2.02°, and consequently improved cell adhesion. The degradation rate of all scaffolds was about 20%, and chemical analysis (FTIR) confirmed the presence of 58 S in zein nanoscale mats. Tensile analysis presented that applying bioactive glass rescued Young's modulus from 0.34 ± 0.07 to 0.08 ± 0.009 MPa. Meanwhile, other results revealed that 4%BG scaffolds exhibit desirable properties, being porous, safe, bioactive, and osteogenic. These findings robustly affirm the competence and potential of the manufactured nanofibrous scaffold containing 4%BG for applications in bone tissue engineering. The schematic diagram illustrating different stages of the study, including; zein/BG scaffold synthesis, characterizations and osteogenesis evaluation in vitro and in vivo [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Polymers & the Environment is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10924-024-03432-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 21 StartPage: 462 Subjects: – SubjectFull: Young's modulus Type: general – SubjectFull: Biomedical engineering Type: general – SubjectFull: Cell adhesion Type: general – SubjectFull: Tissue engineering Type: general – SubjectFull: Contact angle Type: general – SubjectFull: Bioactive glasses Type: general – SubjectFull: Zein (Plant protein) Type: general Titles: – TitleFull: Exploring the Zein/58S Bioactive Glass Nanocomposite for Enhanced Bone Tissue Engineering: A Comprehensive Investigation of Structural, Chemical, Biological, and Osteogenic Properties through in Vitro and in Vivo Studies. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Esmaeili Ranjbar, Faezeh – PersonEntity: Name: NameFull: Mohandesnezhad, Sanam – PersonEntity: Name: NameFull: Mirzaei-Parsa, Mohamad Javad – PersonEntity: Name: NameFull: Asadi, Fatemeh – PersonEntity: Name: NameFull: Divanpour, Samalireza – PersonEntity: Name: NameFull: Karimabad, Mojgan Noroozi – PersonEntity: Name: NameFull: Vatanparast, Mahboubeh – PersonEntity: Name: NameFull: Mirzaei, Mohammad Reza – PersonEntity: Name: NameFull: Hassanshahi, Gholamhossein – PersonEntity: Name: NameFull: Tayebi, Lobat – PersonEntity: Name: NameFull: Esmaeili Ranjbar, Afsaneh IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 15662543 Numbering: – Type: volume Value: 33 – Type: issue Value: 1 Titles: – TitleFull: Journal of Polymers & the Environment Type: main |
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