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Involvement of AhR/ARNT in tolerance to deltamethrin by regulating the expression of TcGSTs6 in Tribolium castaneum.

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Title: Involvement of AhR/ARNT in tolerance to deltamethrin by regulating the expression of TcGSTs6 in Tribolium castaneum.
Authors: Luo, Zhichao1 (AUTHOR), Chen, Haoting2 (AUTHOR), Guan, Daojie1 (AUTHOR), Hang, Chen1 (AUTHOR), Wang, Zhichao1 (AUTHOR), Ge, Huichen1 (AUTHOR), Qian, Kun1 (AUTHOR), Wang, Jianjun1 (AUTHOR) wangjj@yzu.edu.cn
Source: Pesticide Biochemistry & Physiology. Mar2025, Vol. 208, pN.PAG-N.PAG. 1p.
Subject Terms: Xenobiotics, Aryl hydrocarbon receptors, Red flour beetle, Deltamethrin, Genetic transcription regulation
Abstract: It is well known that the transcription factors aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) play important roles in the detoxification of xenobiotic compounds including insecticides. In this study, TcAhR and TcARNT were cloned from Tribolium castaneum. RT-qPCR analysis revealed that TcAhR and TcARNT were highly expressed in brain and epidermis of female adults and upregulated after exposure of the adults to deltamethrin. Knockdown of TcAhR and TcARNT increased the susceptibility of T. castaneum to deltamethrin and decreased the enzymatic activity of Glutathione S-transferase (GST). Consistently, knockdown of TcAhR and TcARNT led to the downregulation of TcGSTs6 and suppressed the induction of TcGSTs6 by deltamethrin treatment. Increased susceptibility of T. castaneum to deltamethrin was also observed after knockdown of TcGSTs6. Further dual-luciferase reporter assay revealed that co-overexpression of TcAhR and TcARNT in Sf9 cells significantly enhanced the promoter activity of TcGSTs6. These results provided insights into the function of insect AhR and ARNT as well as the regulatory mechanisms of insect GSTs. [Display omitted] • Full-length ORFs of TcAhR and TcARNT were cloned from T. castaneum. • Knockdown of TcAhR and TcARNT decreased tolerance of T. castaneum to deltamethrin. • TcGSTs6 was upregulated by deltamethrin treatment. • Knockdown of TcGSTs6 decreased tolerance of T. castaneum to deltamethrin. • TcAhR and TcARNT regulated the expression of TcGSTs6. [ABSTRACT FROM AUTHOR]
Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Involvement of AhR/ARNT in tolerance to deltamethrin by regulating the expression of TcGSTs6 in Tribolium castaneum.
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  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Luo%2C+Zhichao%22">Luo, Zhichao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Haoting%22">Chen, Haoting</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Guan%2C+Daojie%22">Guan, Daojie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hang%2C+Chen%22">Hang, Chen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhichao%22">Wang, Zhichao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ge%2C+Huichen%22">Ge, Huichen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qian%2C+Kun%22">Qian, Kun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Jianjun%22">Wang, Jianjun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wangjj@yzu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. Mar2025, Vol. 208, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subject Terms
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  Data: <searchLink fieldCode="DE" term="%22Xenobiotics%22">Xenobiotics</searchLink><br /><searchLink fieldCode="DE" term="%22Aryl+hydrocarbon+receptors%22">Aryl hydrocarbon receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Red+flour+beetle%22">Red flour beetle</searchLink><br /><searchLink fieldCode="DE" term="%22Deltamethrin%22">Deltamethrin</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+transcription+regulation%22">Genetic transcription regulation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: It is well known that the transcription factors aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) play important roles in the detoxification of xenobiotic compounds including insecticides. In this study, TcAhR and TcARNT were cloned from Tribolium castaneum. RT-qPCR analysis revealed that TcAhR and TcARNT were highly expressed in brain and epidermis of female adults and upregulated after exposure of the adults to deltamethrin. Knockdown of TcAhR and TcARNT increased the susceptibility of T. castaneum to deltamethrin and decreased the enzymatic activity of Glutathione S-transferase (GST). Consistently, knockdown of TcAhR and TcARNT led to the downregulation of TcGSTs6 and suppressed the induction of TcGSTs6 by deltamethrin treatment. Increased susceptibility of T. castaneum to deltamethrin was also observed after knockdown of TcGSTs6. Further dual-luciferase reporter assay revealed that co-overexpression of TcAhR and TcARNT in Sf9 cells significantly enhanced the promoter activity of TcGSTs6. These results provided insights into the function of insect AhR and ARNT as well as the regulatory mechanisms of insect GSTs. [Display omitted] • Full-length ORFs of TcAhR and TcARNT were cloned from T. castaneum. • Knockdown of TcAhR and TcARNT decreased tolerance of T. castaneum to deltamethrin. • TcGSTs6 was upregulated by deltamethrin treatment. • Knockdown of TcGSTs6 decreased tolerance of T. castaneum to deltamethrin. • TcAhR and TcARNT regulated the expression of TcGSTs6. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.pestbp.2024.106281
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Xenobiotics
        Type: general
      – SubjectFull: Aryl hydrocarbon receptors
        Type: general
      – SubjectFull: Red flour beetle
        Type: general
      – SubjectFull: Deltamethrin
        Type: general
      – SubjectFull: Genetic transcription regulation
        Type: general
    Titles:
      – TitleFull: Involvement of AhR/ARNT in tolerance to deltamethrin by regulating the expression of TcGSTs6 in Tribolium castaneum.
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            NameFull: Luo, Zhichao
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            NameFull: Chen, Haoting
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            – D: 01
              M: 03
              Text: Mar2025
              Type: published
              Y: 2025
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              Value: 208
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