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The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum.

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Title: The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum.
Authors: Ji, Caihong1,2 (AUTHOR), Guan, Daojie1 (AUTHOR), Chen, Haoting3 (AUTHOR), Luo, Zhichao1 (AUTHOR), Jian, Chengyun1 (AUTHOR), Wang, Zhichao1 (AUTHOR), Ge, Huichen1 (AUTHOR), Qian, Kun1 (AUTHOR), Wang, Jianjun1 (AUTHOR) wangjj@yzu.edu.cn
Source: Pesticide Biochemistry & Physiology. May2025, Vol. 210, pN.PAG-N.PAG. 1p.
Subject Terms: Red flour beetle, Transcription factors, RNA interference, Small interfering RNA, RNA analysis
Abstract: As an essential component of the thioredoxin system, thioredoxin reductase (TrxR) plays an important role in maintaining redox homeostasis in mammalian cells, however, functional characterization of insect TrxRs is still limited. In this study, full-length cDNAs of TcTrxR1 and TcTrxR2 were cloned from the red flour beetle, Tribolium castaneum. Sequence analysis revealed the highly conserved active site motifs CVNVGC and CCS at the N-terminal and C-terminal of TcTrxR1, respectively, whereas TcTrxR2 lacks these two conserved motifs. Analysis of the spatio-temporal expression pattern by RT-qPCR showed that the expression of TcTrxR1 was the highest in 1-day-old larva and brain, and TcTrxR2 was highly expressed in eggs and fat body, respectively. Further functional analysis by RNA interference (RNAi) revealed that knockdown of TcTrxR1 and TcTrxR2 at the larval stage led to 100 % and 98.67 % mortality of larvae beetles, and pupal RNAi of TcTrxR1 and TcTrxR2 resulted in decreased eclosion rates as well as failure of the female adults to lay eggs. Additionally, injection of dsTcTrxR2 decreased the tolerance of beetles to deltamethrin, whereas knockdown of TcTrxR1 significantly increased the tolerance of beetles to deltamethrin. Notably, knockdown of TcTrxR1 significantly upregulated the expression of TcCYP6BQ2 , TcCYP6BQ4 and TcCYP6BQ7 , and led to nuclear translocation of transcription factor CncC, a major regulator of detoxification in insects. These findings provide insights into the function of insect TrxRs as well as the regulatory mechanisms of CncC, and have applied implications for the RNAi-based insect pest control. [Display omitted] • Full-length cDNAs of TcTrxR1 and TcTrxR2 were cloned from T. castaneum. • Larval RNAi of TcTrxR1 and TcTrxR2 led to high mortality of larvae beetles. • Pupal RNAi of TcTrxR1 and TcTrxR2 resulted in the failure of the female adults to lay eggs. • Knockdown of TcTrxR1 increased the tolerance of beetles to deltamethrin. • Knockdown of TcTrxR1 led to nuclear translocation of transcription factor CncC. [ABSTRACT FROM AUTHOR]
Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum.
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  Data: <searchLink fieldCode="AR" term="%22Ji%2C+Caihong%22">Ji, Caihong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Guan%2C+Daojie%22">Guan, Daojie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Haoting%22">Chen, Haoting</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Luo%2C+Zhichao%22">Luo, Zhichao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jian%2C+Chengyun%22">Jian, Chengyun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhichao%22">Wang, Zhichao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ge%2C+Huichen%22">Ge, Huichen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qian%2C+Kun%22">Qian, Kun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Jianjun%22">Wang, Jianjun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wangjj@yzu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. May2025, Vol. 210, pN.PAG-N.PAG. 1p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Red+flour+beetle%22">Red flour beetle</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+interference%22">RNA interference</searchLink><br /><searchLink fieldCode="DE" term="%22Small+interfering+RNA%22">Small interfering RNA</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+analysis%22">RNA analysis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: As an essential component of the thioredoxin system, thioredoxin reductase (TrxR) plays an important role in maintaining redox homeostasis in mammalian cells, however, functional characterization of insect TrxRs is still limited. In this study, full-length cDNAs of TcTrxR1 and TcTrxR2 were cloned from the red flour beetle, Tribolium castaneum. Sequence analysis revealed the highly conserved active site motifs CVNVGC and CCS at the N-terminal and C-terminal of TcTrxR1, respectively, whereas TcTrxR2 lacks these two conserved motifs. Analysis of the spatio-temporal expression pattern by RT-qPCR showed that the expression of TcTrxR1 was the highest in 1-day-old larva and brain, and TcTrxR2 was highly expressed in eggs and fat body, respectively. Further functional analysis by RNA interference (RNAi) revealed that knockdown of TcTrxR1 and TcTrxR2 at the larval stage led to 100 % and 98.67 % mortality of larvae beetles, and pupal RNAi of TcTrxR1 and TcTrxR2 resulted in decreased eclosion rates as well as failure of the female adults to lay eggs. Additionally, injection of dsTcTrxR2 decreased the tolerance of beetles to deltamethrin, whereas knockdown of TcTrxR1 significantly increased the tolerance of beetles to deltamethrin. Notably, knockdown of TcTrxR1 significantly upregulated the expression of TcCYP6BQ2 , TcCYP6BQ4 and TcCYP6BQ7 , and led to nuclear translocation of transcription factor CncC, a major regulator of detoxification in insects. These findings provide insights into the function of insect TrxRs as well as the regulatory mechanisms of CncC, and have applied implications for the RNAi-based insect pest control. [Display omitted] • Full-length cDNAs of TcTrxR1 and TcTrxR2 were cloned from T. castaneum. • Larval RNAi of TcTrxR1 and TcTrxR2 led to high mortality of larvae beetles. • Pupal RNAi of TcTrxR1 and TcTrxR2 resulted in the failure of the female adults to lay eggs. • Knockdown of TcTrxR1 increased the tolerance of beetles to deltamethrin. • Knockdown of TcTrxR1 led to nuclear translocation of transcription factor CncC. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1016/j.pestbp.2025.106390
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Red flour beetle
        Type: general
      – SubjectFull: Transcription factors
        Type: general
      – SubjectFull: RNA interference
        Type: general
      – SubjectFull: Small interfering RNA
        Type: general
      – SubjectFull: RNA analysis
        Type: general
    Titles:
      – TitleFull: The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum.
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            NameFull: Ji, Caihong
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            NameFull: Guan, Daojie
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            – D: 01
              M: 05
              Text: May2025
              Type: published
              Y: 2025
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              Value: 210
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