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Food Waste to Wellness: A Grape Pomace Blend in Gastric Cancer Prevention.

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Title: Food Waste to Wellness: A Grape Pomace Blend in Gastric Cancer Prevention.
Authors: Micucci, Matteo1 (AUTHOR), Battistelli, Michela1 (AUTHOR) michela.battistelli@uniurb.it, Burattini, Sabrina1 (AUTHOR), Mari, Michele1 (AUTHOR), Retini, Michele1 (AUTHOR), Osman, Riham1 (AUTHOR), Versari, Ilaria2 (AUTHOR), Stella, Anna Bartoletti3 (AUTHOR), Gianfanti, Federico1 (AUTHOR) federico.gianfanti@uniurb.it, Udodinma, Jude Okeke1 (AUTHOR), Onesimo, Francesco1 (AUTHOR), Riela, Serena4 (AUTHOR), Canale, Matteo5 (AUTHOR), Piccionello, Antonio Palumbo6 (AUTHOR), Faenza, Irene2 (AUTHOR), Salucci, Sara2 (AUTHOR)
Source: Food Science & Nutrition. Jun2025, Vol. 13 Issue 6, p1-17. 17p.
Subject Terms: Apoptotic bodies, Circular economy, Stomach cancer, Carboxylic acids, Succinic acid
Abstract: This study investigates the chemopreventive potential of a grape pomace extract (GPE) derived from Verdicchio grapes, aligning with circular economy principles to repurpose winery waste into a nutraceutical targeting gastric cancer prevention. Soxhlet extraction yielded a bioactive‐rich extract. Comprehensive chemical characterization via HPLC/ESI/Q‐TOF identified 39 metabolites spanning key chemical classes. Anthocyanins were predominant, with malvidin glucoside (12,546 mg/kg DM; 36.3%), malvidin coumaroyl glucoside (9941 mg/kg DM; 28.8%), and malvidin acetylglucoside (7189 mg/kg DM; 20.8%) as the most abundant compounds. Carboxylic acids included tartaric, malic, isocitric, aconitic, and succinic acids, while lipid molecules, such as phytosphingosine and stearic acid, and amino acids like proline, valine, leucine, and phenylalanine further enriched the extract's chemical heterogeneity. Biological evaluations revealed GPE's selective cytotoxicity against AGS (IC50: 13.64 μg/mL) and KATO III (IC50: 7.11 μg/mL) gastric cancer cells, sparing GES‐1 cells. Mechanistically, GPE‐induced apoptosis through caspase‐3 activation and mitochondrial dysfunction, as evidenced by inner membrane disruption and cardiolipin peroxidation. TEM and CLSM morphological analyses revealed hallmark apoptotic features, including chromatin condensation, micronuclei formation, and apoptotic bodies. Additionally, GPE impaired autophagy, marked by Beclin‐1 downregulation and LC3B‐II upregulation. The accumulation of degradative vacuoles indicated disrupted autophagosome clearance, shifting autophagy from a survival mechanism to a cell death‐promoting pathway. These findings highlight GPE's dual impact on apoptosis and autophagy in gastric cancer cells, underscoring its potential as a dietary intervention for gastric cancer prevention. [ABSTRACT FROM AUTHOR]
Copyright of Food Science & Nutrition is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Food Waste to Wellness: A Grape Pomace Blend in Gastric Cancer Prevention.
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  Data: <searchLink fieldCode="AR" term="%22Micucci%2C+Matteo%22">Micucci, Matteo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Battistelli%2C+Michela%22">Battistelli, Michela</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> michela.battistelli@uniurb.it</i><br /><searchLink fieldCode="AR" term="%22Burattini%2C+Sabrina%22">Burattini, Sabrina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mari%2C+Michele%22">Mari, Michele</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Retini%2C+Michele%22">Retini, Michele</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Osman%2C+Riham%22">Osman, Riham</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Versari%2C+Ilaria%22">Versari, Ilaria</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stella%2C+Anna+Bartoletti%22">Stella, Anna Bartoletti</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gianfanti%2C+Federico%22">Gianfanti, Federico</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> federico.gianfanti@uniurb.it</i><br /><searchLink fieldCode="AR" term="%22Udodinma%2C+Jude+Okeke%22">Udodinma, Jude Okeke</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Onesimo%2C+Francesco%22">Onesimo, Francesco</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Riela%2C+Serena%22">Riela, Serena</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Canale%2C+Matteo%22">Canale, Matteo</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Piccionello%2C+Antonio+Palumbo%22">Piccionello, Antonio Palumbo</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Faenza%2C+Irene%22">Faenza, Irene</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Salucci%2C+Sara%22">Salucci, Sara</searchLink><relatesTo>2</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="DE" term="%22Apoptotic+bodies%22">Apoptotic bodies</searchLink><br /><searchLink fieldCode="DE" term="%22Circular+economy%22">Circular economy</searchLink><br /><searchLink fieldCode="DE" term="%22Stomach+cancer%22">Stomach cancer</searchLink><br /><searchLink fieldCode="DE" term="%22Carboxylic+acids%22">Carboxylic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Succinic+acid%22">Succinic acid</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: This study investigates the chemopreventive potential of a grape pomace extract (GPE) derived from Verdicchio grapes, aligning with circular economy principles to repurpose winery waste into a nutraceutical targeting gastric cancer prevention. Soxhlet extraction yielded a bioactive‐rich extract. Comprehensive chemical characterization via HPLC/ESI/Q‐TOF identified 39 metabolites spanning key chemical classes. Anthocyanins were predominant, with malvidin glucoside (12,546 mg/kg DM; 36.3%), malvidin coumaroyl glucoside (9941 mg/kg DM; 28.8%), and malvidin acetylglucoside (7189 mg/kg DM; 20.8%) as the most abundant compounds. Carboxylic acids included tartaric, malic, isocitric, aconitic, and succinic acids, while lipid molecules, such as phytosphingosine and stearic acid, and amino acids like proline, valine, leucine, and phenylalanine further enriched the extract's chemical heterogeneity. Biological evaluations revealed GPE's selective cytotoxicity against AGS (IC50: 13.64 μg/mL) and KATO III (IC50: 7.11 μg/mL) gastric cancer cells, sparing GES‐1 cells. Mechanistically, GPE‐induced apoptosis through caspase‐3 activation and mitochondrial dysfunction, as evidenced by inner membrane disruption and cardiolipin peroxidation. TEM and CLSM morphological analyses revealed hallmark apoptotic features, including chromatin condensation, micronuclei formation, and apoptotic bodies. Additionally, GPE impaired autophagy, marked by Beclin‐1 downregulation and LC3B‐II upregulation. The accumulation of degradative vacuoles indicated disrupted autophagosome clearance, shifting autophagy from a survival mechanism to a cell death‐promoting pathway. These findings highlight GPE's dual impact on apoptosis and autophagy in gastric cancer cells, underscoring its potential as a dietary intervention for gastric cancer prevention. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Food Science & Nutrition is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1002/fsn3.70350
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        Text: English
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      – SubjectFull: Stomach cancer
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      – SubjectFull: Carboxylic acids
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      – SubjectFull: Succinic acid
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      – TitleFull: Food Waste to Wellness: A Grape Pomace Blend in Gastric Cancer Prevention.
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