Characterizations and transcriptional response of peroxiredoxin genes, TcPrx6a and TcPrx6b, to paraquat and deltamethrin exposures in Tribolium castaneum.
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| Title: | Characterizations and transcriptional response of peroxiredoxin genes, TcPrx6a and TcPrx6b, to paraquat and deltamethrin exposures in Tribolium castaneum. |
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| Authors: | Hang, Chen1 (AUTHOR), Wei, Jiaping1 (AUTHOR), Guan, Daojie1 (AUTHOR), Wang, Zhichao1 (AUTHOR), Ge, Huichen1 (AUTHOR), Qian, Kun1 (AUTHOR), Wang, Jianjun1,2 (AUTHOR) wangjj@yzu.edu.cn |
| Source: | Pesticide Biochemistry & Physiology. Sep2025, Vol. 213, pN.PAG-N.PAG. 1p. |
| Subject Terms: | *Paraquat, Red flour beetle, Transcription factors, Deltamethrin, Genetic transcription regulation |
| Abstract: | Prx6 attracts particular attention because of its ability to reduce the widest range of peroxidative substrates among the peroxiredoxin (Prx) family. In this study, TcPrx6a and TcPrx6b were cloned from Tribolium castaneum. RT-qPCR analysis revealed that TcPrx6a and TcPrx6b were highly expressed in fat body and head, respectively, and both were upregulated after exposure of the beetles to paraquat and deltamethrin. Knockdown of TcPrx6a and TcPrx6b decreased the tolerance of T. castaneum to paraquat-induced oxidative stress and deltamethrin, and enhanced deltamethrin-induced accumulation of H 2 O 2. Interestingly, knockdown of TcPrx6a and TcPrx6b negatively affected the pupation and eclosion rates as well as fecundity and fertility of beetles. Additionally, both RNAi and the dual-luciferase reporter assay in Sf9 cells revealed that the transcription of TcPrx6a and TcPrx6b was potentially regulated by transcription factor CncC. These results provided insights into the function of TcPrx6a and TcPrx6b as well as the transcriptional regulation of insect Prx6s. [Display omitted] • TcPrx6a and TcPrx6b were highly expressed in fat body and head, respectively. • TcPrx6s were upregulated after exposure of beetles to various stressors. • Knockdown of TcPrx6s decreased tolerance of beetles to paraquat and deltamethrin. • Knockdown of TcPrx6s negatively affected development and reproduction. • TcCncC regulated the expression of TcPrx6a and TcPrx6b. [ABSTRACT FROM AUTHOR] |
| Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 186994953 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Characterizations and transcriptional response of peroxiredoxin genes, TcPrx6a and TcPrx6b, to paraquat and deltamethrin exposures in Tribolium castaneum. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Hang%2C+Chen%22">Hang, Chen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wei%2C+Jiaping%22">Wei, Jiaping</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Guan%2C+Daojie%22">Guan, Daojie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhichao%22">Wang, Zhichao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ge%2C+Huichen%22">Ge, Huichen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qian%2C+Kun%22">Qian, Kun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Jianjun%22">Wang, Jianjun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> wangjj@yzu.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. Sep2025, Vol. 213, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22Paraquat%22">Paraquat</searchLink><br /><searchLink fieldCode="DE" term="%22Red+flour+beetle%22">Red flour beetle</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Deltamethrin%22">Deltamethrin</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+transcription+regulation%22">Genetic transcription regulation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Prx6 attracts particular attention because of its ability to reduce the widest range of peroxidative substrates among the peroxiredoxin (Prx) family. In this study, TcPrx6a and TcPrx6b were cloned from Tribolium castaneum. RT-qPCR analysis revealed that TcPrx6a and TcPrx6b were highly expressed in fat body and head, respectively, and both were upregulated after exposure of the beetles to paraquat and deltamethrin. Knockdown of TcPrx6a and TcPrx6b decreased the tolerance of T. castaneum to paraquat-induced oxidative stress and deltamethrin, and enhanced deltamethrin-induced accumulation of H 2 O 2. Interestingly, knockdown of TcPrx6a and TcPrx6b negatively affected the pupation and eclosion rates as well as fecundity and fertility of beetles. Additionally, both RNAi and the dual-luciferase reporter assay in Sf9 cells revealed that the transcription of TcPrx6a and TcPrx6b was potentially regulated by transcription factor CncC. These results provided insights into the function of TcPrx6a and TcPrx6b as well as the transcriptional regulation of insect Prx6s. [Display omitted] • TcPrx6a and TcPrx6b were highly expressed in fat body and head, respectively. • TcPrx6s were upregulated after exposure of beetles to various stressors. • Knockdown of TcPrx6s decreased tolerance of beetles to paraquat and deltamethrin. • Knockdown of TcPrx6s negatively affected development and reproduction. • TcCncC regulated the expression of TcPrx6a and TcPrx6b. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.pestbp.2025.106493 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Paraquat Type: general – SubjectFull: Red flour beetle Type: general – SubjectFull: Transcription factors Type: general – SubjectFull: Deltamethrin Type: general – SubjectFull: Genetic transcription regulation Type: general Titles: – TitleFull: Characterizations and transcriptional response of peroxiredoxin genes, TcPrx6a and TcPrx6b, to paraquat and deltamethrin exposures in Tribolium castaneum. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hang, Chen – PersonEntity: Name: NameFull: Wei, Jiaping – PersonEntity: Name: NameFull: Guan, Daojie – PersonEntity: Name: NameFull: Wang, Zhichao – PersonEntity: Name: NameFull: Ge, Huichen – PersonEntity: Name: NameFull: Qian, Kun – PersonEntity: Name: NameFull: Wang, Jianjun IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 00483575 Numbering: – Type: volume Value: 213 Titles: – TitleFull: Pesticide Biochemistry & Physiology Type: main |
| ResultId | 1 |
