Phytochemical Profiling, Antioxidant Capacity, and the Antidiabetic Potential of Atriplex halimus Extracts: UHPLC–MS/MS Analysis, In Vitro Enzyme Inhibition Assays, and Molecular Docking Studies.
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| Title: | Phytochemical Profiling, Antioxidant Capacity, and the Antidiabetic Potential of Atriplex halimus Extracts: UHPLC–MS/MS Analysis, In Vitro Enzyme Inhibition Assays, and Molecular Docking Studies. |
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| Authors: | Roubi, Mohammed1 (AUTHOR) mohammed.roubi1@ump.ac.ma, Daoudi, Nour Elhouda1,2 (AUTHOR), Dalli, Mohammed1,2 (AUTHOR), Azizi, Salah‐eddine1,2 (AUTHOR), Mahdi, Youness1 (AUTHOR), Mothana, Ramzi A.3 (AUTHOR) rmothana@ksu.edu.sa, Al‐Yousef, Hanan M.3 (AUTHOR), Hawwal, Mohammed F.3 (AUTHOR), Kandsi, Fahd1 (AUTHOR), Conte, Raffaele4 (AUTHOR), Gseyra, Nadia1 (AUTHOR) |
| Source: | Food Science & Nutrition. Aug2025, Vol. 13 Issue 8, p1-12. 12p. |
| Subject Terms: | Atriplex, Molecular docking, Oxidant status, Bioactive compounds, Insulin sensitivity, Biological assay, Liquid chromatography-mass spectrometry |
| Abstract: | Atriplex halimus (L.) is known to be characterized by numerous pharmacological prospects comprising antioxidant, anticancer, antibacterial, and antidiabetic properties. The core objective of the present study is to harness the chemical composition of aqueous extract (EA), hydroethanolic extract (EHA), and ethanolic extract (EE), followed by the investigation of the antidiabetic potential of A. halimus extracts on two important targets, porcine proteins (α‐amylase and hemoglobin). A molecular docking study was adopted to computationally identify the bioactive compounds responsible for the observed antidiabetic effect. Phytochemical profiling using UHPLC–MS/MS identified 21 compounds across the three extracts, with trans‐cinnamic acid being the most abundant in all. The extracts exhibited significant inhibitory effects on both α‐amylase and hemoglobin glycation, with IC50 values of 1.89, 3.33, and 2.07 mg/mL (for EA, EHA, and EE, respectively) against α‐amylase and 0.29, 0.31, and 0.41 mg/mL against glycation, demonstrating a significant inhibitory impact of A. halimus on both target proteins. With regard to the in silico computational study, it has been demonstrated that both catechin and epigallocatechin emerge as the most active compounds, displaying high binding activity compared to acarbose, a standard antidiabetic drug. These findings highlight the rich phytochemical profile and strong antidiabetic potential of A. halimus extracts, supporting their development as natural therapeutic agents for diabetes management. [ABSTRACT FROM AUTHOR] |
| Copyright of Food Science & Nutrition is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 187526227 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Phytochemical Profiling, Antioxidant Capacity, and the Antidiabetic Potential of Atriplex halimus Extracts: UHPLC–MS/MS Analysis, In Vitro Enzyme Inhibition Assays, and Molecular Docking Studies. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Roubi%2C+Mohammed%22">Roubi, Mohammed</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mohammed.roubi1@ump.ac.ma</i><br /><searchLink fieldCode="AR" term="%22Daoudi%2C+Nour+Elhouda%22">Daoudi, Nour Elhouda</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dalli%2C+Mohammed%22">Dalli, Mohammed</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Azizi%2C+Salah‐eddine%22">Azizi, Salah‐eddine</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mahdi%2C+Youness%22">Mahdi, Youness</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mothana%2C+Ramzi+A%2E%22">Mothana, Ramzi A.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> rmothana@ksu.edu.sa</i><br /><searchLink fieldCode="AR" term="%22Al‐Yousef%2C+Hanan+M%2E%22">Al‐Yousef, Hanan M.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hawwal%2C+Mohammed+F%2E%22">Hawwal, Mohammed F.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kandsi%2C+Fahd%22">Kandsi, Fahd</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Conte%2C+Raffaele%22">Conte, Raffaele</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gseyra%2C+Nadia%22">Gseyra, Nadia</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Food+Science+%26+Nutrition%22">Food Science & Nutrition</searchLink>. Aug2025, Vol. 13 Issue 8, p1-12. 12p. – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Atriplex%22">Atriplex</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+docking%22">Molecular docking</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidant+status%22">Oxidant status</searchLink><br /><searchLink fieldCode="DE" term="%22Bioactive+compounds%22">Bioactive compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Insulin+sensitivity%22">Insulin sensitivity</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+assay%22">Biological assay</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography-mass+spectrometry%22">Liquid chromatography-mass spectrometry</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Atriplex halimus (L.) is known to be characterized by numerous pharmacological prospects comprising antioxidant, anticancer, antibacterial, and antidiabetic properties. The core objective of the present study is to harness the chemical composition of aqueous extract (EA), hydroethanolic extract (EHA), and ethanolic extract (EE), followed by the investigation of the antidiabetic potential of A. halimus extracts on two important targets, porcine proteins (α‐amylase and hemoglobin). A molecular docking study was adopted to computationally identify the bioactive compounds responsible for the observed antidiabetic effect. Phytochemical profiling using UHPLC–MS/MS identified 21 compounds across the three extracts, with trans‐cinnamic acid being the most abundant in all. The extracts exhibited significant inhibitory effects on both α‐amylase and hemoglobin glycation, with IC50 values of 1.89, 3.33, and 2.07 mg/mL (for EA, EHA, and EE, respectively) against α‐amylase and 0.29, 0.31, and 0.41 mg/mL against glycation, demonstrating a significant inhibitory impact of A. halimus on both target proteins. With regard to the in silico computational study, it has been demonstrated that both catechin and epigallocatechin emerge as the most active compounds, displaying high binding activity compared to acarbose, a standard antidiabetic drug. These findings highlight the rich phytochemical profile and strong antidiabetic potential of A. halimus extracts, supporting their development as natural therapeutic agents for diabetes management. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Food Science & Nutrition is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/fsn3.70745 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1 Subjects: – SubjectFull: Atriplex Type: general – SubjectFull: Molecular docking Type: general – SubjectFull: Oxidant status Type: general – SubjectFull: Bioactive compounds Type: general – SubjectFull: Insulin sensitivity Type: general – SubjectFull: Biological assay Type: general – SubjectFull: Liquid chromatography-mass spectrometry Type: general Titles: – TitleFull: Phytochemical Profiling, Antioxidant Capacity, and the Antidiabetic Potential of Atriplex halimus Extracts: UHPLC–MS/MS Analysis, In Vitro Enzyme Inhibition Assays, and Molecular Docking Studies. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Roubi, Mohammed – PersonEntity: Name: NameFull: Daoudi, Nour Elhouda – PersonEntity: Name: NameFull: Dalli, Mohammed – PersonEntity: Name: NameFull: Azizi, Salah‐eddine – PersonEntity: Name: NameFull: Mahdi, Youness – PersonEntity: Name: NameFull: Mothana, Ramzi A. – PersonEntity: Name: NameFull: Al‐Yousef, Hanan M. – PersonEntity: Name: NameFull: Hawwal, Mohammed F. – PersonEntity: Name: NameFull: Kandsi, Fahd – PersonEntity: Name: NameFull: Conte, Raffaele – PersonEntity: Name: NameFull: Gseyra, Nadia IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 20487177 Numbering: – Type: volume Value: 13 – Type: issue Value: 8 Titles: – TitleFull: Food Science & Nutrition Type: main |
| ResultId | 1 |
