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Molecular characterization of Spodoptera frugiperda nose resistant to fluoxetine protein 6 and its putative involvement in tolerance to cyantraniliprole.

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Title: Molecular characterization of Spodoptera frugiperda nose resistant to fluoxetine protein 6 and its putative involvement in tolerance to cyantraniliprole.
Authors: Wei, Jiaping1 (AUTHOR), Liu, Changpeng1 (AUTHOR), Guan, Daojie1 (AUTHOR), Wang, Zhichao1 (AUTHOR), Ge, Huichen1 (AUTHOR), Li, Hai1 (AUTHOR), Zhang, Hainan1 (AUTHOR), Qian, Kun1 (AUTHOR), Wang, Jianjun1,2 (AUTHOR) wangjj@yzu.edu.cn
Source: Pesticide Biochemistry & Physiology. Nov2025, Vol. 214, pN.PAG-N.PAG. 1p.
Subject Terms: *Insecticide resistance, Fall armyworm, Gene expression, Chlorantraniliprole, RNA sequencing
Abstract: Spodoptera frugiperda (FAW) is a notorious polyphagous pest that has developed resistance to various insecticides including diamide insecticides. Our previous study established a FAW cyantraniliprole-resistant (SfCYAN-R) strain by laboratory resistance selection of susceptible strain (SfCYAN-S), however, the potential resistance mechanisms of FAW to cyantraniliprole remain unclear. In this study, SfNrf6 encoding nose resistant to fluoxetine (Nrf) protein 6 was identified to be upregulated in SfCYAN-R strain compared with SfCYAN-S strain based on RNA-Seq data and RT-qPCR. The cDNA of Nrf6 was 2094 base pairs in length and encoded a protein of 697 amino acids. Sequence analysis revealed that SfNrf6 contained the conserved Nrf domain as well as acyltransferase domain, and shared high amino acid identity with Bombyx mori homologue. RT-qPCR analysis revealed that SfNrf6 was highly expressed in the midgut, and was upregulated at 1 h and 2 h after exposure of the third instar larvae to cyantraniliprole. Notably, knockdown of SfNrf6 significantly increased the susceptibility of FAW to cyantraniliprole. Furthermore, cytotoxicity assay demonstrated that overexpression of SfNrf6 enhanced Sf9 cell viability under cyantraniliprole treatment. These results provided preliminary evidence that SfNrf6 was involved in cyantraniliprole tolerance in FAW, which had applied implications for FAW resistance management. [Display omitted] • Nrf6 was up-regulated in cyantraniliprole-resistant strain of FAW. • SfNrf6 specifically expressed in the midgut. • Knockdown of SfNrf6 increased FAW susceptibility to cyantraniliprole. • Overexpression of SfNrf6 increased the viability of Sf9 cells treated with cyantraniliprole. [ABSTRACT FROM AUTHOR]
Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Molecular characterization of Spodoptera frugiperda nose resistant to fluoxetine protein 6 and its putative involvement in tolerance to cyantraniliprole.
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  Data: <searchLink fieldCode="AR" term="%22Wei%2C+Jiaping%22">Wei, Jiaping</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Changpeng%22">Liu, Changpeng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Guan%2C+Daojie%22">Guan, Daojie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhichao%22">Wang, Zhichao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ge%2C+Huichen%22">Ge, Huichen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Hai%22">Li, Hai</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Hainan%22">Zhang, Hainan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qian%2C+Kun%22">Qian, Kun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Jianjun%22">Wang, Jianjun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> wangjj@yzu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. Nov2025, Vol. 214, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Insecticide+resistance%22">Insecticide resistance</searchLink><br /><searchLink fieldCode="DE" term="%22Fall+armyworm%22">Fall armyworm</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Chlorantraniliprole%22">Chlorantraniliprole</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Spodoptera frugiperda (FAW) is a notorious polyphagous pest that has developed resistance to various insecticides including diamide insecticides. Our previous study established a FAW cyantraniliprole-resistant (SfCYAN-R) strain by laboratory resistance selection of susceptible strain (SfCYAN-S), however, the potential resistance mechanisms of FAW to cyantraniliprole remain unclear. In this study, SfNrf6 encoding nose resistant to fluoxetine (Nrf) protein 6 was identified to be upregulated in SfCYAN-R strain compared with SfCYAN-S strain based on RNA-Seq data and RT-qPCR. The cDNA of Nrf6 was 2094 base pairs in length and encoded a protein of 697 amino acids. Sequence analysis revealed that SfNrf6 contained the conserved Nrf domain as well as acyltransferase domain, and shared high amino acid identity with Bombyx mori homologue. RT-qPCR analysis revealed that SfNrf6 was highly expressed in the midgut, and was upregulated at 1 h and 2 h after exposure of the third instar larvae to cyantraniliprole. Notably, knockdown of SfNrf6 significantly increased the susceptibility of FAW to cyantraniliprole. Furthermore, cytotoxicity assay demonstrated that overexpression of SfNrf6 enhanced Sf9 cell viability under cyantraniliprole treatment. These results provided preliminary evidence that SfNrf6 was involved in cyantraniliprole tolerance in FAW, which had applied implications for FAW resistance management. [Display omitted] • Nrf6 was up-regulated in cyantraniliprole-resistant strain of FAW. • SfNrf6 specifically expressed in the midgut. • Knockdown of SfNrf6 increased FAW susceptibility to cyantraniliprole. • Overexpression of SfNrf6 increased the viability of Sf9 cells treated with cyantraniliprole. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.pestbp.2025.106602
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Insecticide resistance
        Type: general
      – SubjectFull: Fall armyworm
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Chlorantraniliprole
        Type: general
      – SubjectFull: RNA sequencing
        Type: general
    Titles:
      – TitleFull: Molecular characterization of Spodoptera frugiperda nose resistant to fluoxetine protein 6 and its putative involvement in tolerance to cyantraniliprole.
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            NameFull: Wei, Jiaping
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            – D: 01
              M: 11
              Text: Nov2025
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