View in EDS

High concentrations of Printex 90 carbon black ultrafine particles disturb the epithelial barrier in human primary respiratory mucosa models.

Saved in:
Bibliographic Details
Title: High concentrations of Printex 90 carbon black ultrafine particles disturb the epithelial barrier in human primary respiratory mucosa models.
Authors: Ehret Kasemo, Totta1 (AUTHOR) ehret_t@ukw.de, Oppmann, Maximilian2 (AUTHOR), Dembski, Sofia2 (AUTHOR), Steinke, Maria1 (AUTHOR), Lajtha, Elena1 (AUTHOR), Moratin, Helena1 (AUTHOR), Stöth, Manuel1 (AUTHOR), Scherzad, Agmal1 (AUTHOR), Delaval, Mathilde Noémie3 (AUTHOR), Zimmermann, Ralf3,4 (AUTHOR), Di Bucchianico, Sebastiano3,4 (AUTHOR), Hackenberg, Stephan1 (AUTHOR), Meyer, Till J.1 (AUTHOR)
Source: Environmental Toxicology & Pharmacology. Oct2025, Vol. 119, pN.PAG-N.PAG. 1p.
Subject Terms: *Carbon-black, Respiratory mucosa, DNA damage, Cell adhesion, Cell junctions, Mucociliary system, Nanoparticles, Cytotoxins
Abstract: Airborne pollutants harm human health, but the mechanisms involved remain unclear. Impaired epithelial barrier function is, as in respiratory diseases, one possible pathomechanism. To investigate this, carbon black (CB) as a model for ultrafine particles (UFP), was applied to respiratory mucosa models of primary fibroblasts and epithelial cells cultured at the air-liquid interface (ALI). Models were assessed for the mucociliary phenotype. Cytotoxicity, DNA damage, and barrier integrity were evaluated by the lactate dehydrogenase (LDH) and comet assays, and by transepithelial electrical resistance (TEER) measurements. Cilia movement and ultrastructure, secretory cells, and intact cell-cell contacts were confirmed. Subtle changes were observed: the LDH release had increased 2 h post exposure and barrier disturbance 24 h post exposure was detected, both without mucosal damage or genotoxic effects. Donor-specific differences were present. Barrier disruption without cell detachment or death suggests model feasibility for long-term studies of, e.g. , tissue regeneration or fibrosis following UFP exposure. [Display omitted] • Human upper respiratory system mucosal model with high in vivo -correlation. • Ultrafine particle exposures at the air-liquid interface. • Subtle responses are consistent with minor acute effects in epidemiological data. • Minor effects enable future UFP exposures to study regeneration or fibrosis. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Toxicology & Pharmacology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: GreenFILE
FullText Text:
  Availability: 0
Header DbId: 8gh
DbLabel: GreenFILE
An: 188570892
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: High concentrations of Printex 90 carbon black ultrafine particles disturb the epithelial barrier in human primary respiratory mucosa models.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Ehret+Kasemo%2C+Totta%22">Ehret Kasemo, Totta</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ehret_t@ukw.de</i><br /><searchLink fieldCode="AR" term="%22Oppmann%2C+Maximilian%22">Oppmann, Maximilian</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dembski%2C+Sofia%22">Dembski, Sofia</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Steinke%2C+Maria%22">Steinke, Maria</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lajtha%2C+Elena%22">Lajtha, Elena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Moratin%2C+Helena%22">Moratin, Helena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stöth%2C+Manuel%22">Stöth, Manuel</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Scherzad%2C+Agmal%22">Scherzad, Agmal</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Delaval%2C+Mathilde+Noémie%22">Delaval, Mathilde Noémie</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zimmermann%2C+Ralf%22">Zimmermann, Ralf</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Di+Bucchianico%2C+Sebastiano%22">Di Bucchianico, Sebastiano</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hackenberg%2C+Stephan%22">Hackenberg, Stephan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Meyer%2C+Till+J%2E%22">Meyer, Till J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Environmental+Toxicology+%26+Pharmacology%22">Environmental Toxicology & Pharmacology</searchLink>. Oct2025, Vol. 119, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Carbon-black%22">Carbon-black</searchLink><br /><searchLink fieldCode="DE" term="%22Respiratory+mucosa%22">Respiratory mucosa</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+adhesion%22">Cell adhesion</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+junctions%22">Cell junctions</searchLink><br /><searchLink fieldCode="DE" term="%22Mucociliary+system%22">Mucociliary system</searchLink><br /><searchLink fieldCode="DE" term="%22Nanoparticles%22">Nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Cytotoxins%22">Cytotoxins</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Airborne pollutants harm human health, but the mechanisms involved remain unclear. Impaired epithelial barrier function is, as in respiratory diseases, one possible pathomechanism. To investigate this, carbon black (CB) as a model for ultrafine particles (UFP), was applied to respiratory mucosa models of primary fibroblasts and epithelial cells cultured at the air-liquid interface (ALI). Models were assessed for the mucociliary phenotype. Cytotoxicity, DNA damage, and barrier integrity were evaluated by the lactate dehydrogenase (LDH) and comet assays, and by transepithelial electrical resistance (TEER) measurements. Cilia movement and ultrastructure, secretory cells, and intact cell-cell contacts were confirmed. Subtle changes were observed: the LDH release had increased 2 h post exposure and barrier disturbance 24 h post exposure was detected, both without mucosal damage or genotoxic effects. Donor-specific differences were present. Barrier disruption without cell detachment or death suggests model feasibility for long-term studies of, e.g. , tissue regeneration or fibrosis following UFP exposure. [Display omitted] • Human upper respiratory system mucosal model with high in vivo -correlation. • Ultrafine particle exposures at the air-liquid interface. • Subtle responses are consistent with minor acute effects in epidemiological data. • Minor effects enable future UFP exposures to study regeneration or fibrosis. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Environmental Toxicology & Pharmacology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=8gh&AN=188570892
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.etap.2025.104829
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Carbon-black
        Type: general
      – SubjectFull: Respiratory mucosa
        Type: general
      – SubjectFull: DNA damage
        Type: general
      – SubjectFull: Cell adhesion
        Type: general
      – SubjectFull: Cell junctions
        Type: general
      – SubjectFull: Mucociliary system
        Type: general
      – SubjectFull: Nanoparticles
        Type: general
      – SubjectFull: Cytotoxins
        Type: general
    Titles:
      – TitleFull: High concentrations of Printex 90 carbon black ultrafine particles disturb the epithelial barrier in human primary respiratory mucosa models.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Ehret Kasemo, Totta
      – PersonEntity:
          Name:
            NameFull: Oppmann, Maximilian
      – PersonEntity:
          Name:
            NameFull: Dembski, Sofia
      – PersonEntity:
          Name:
            NameFull: Steinke, Maria
      – PersonEntity:
          Name:
            NameFull: Lajtha, Elena
      – PersonEntity:
          Name:
            NameFull: Moratin, Helena
      – PersonEntity:
          Name:
            NameFull: Stöth, Manuel
      – PersonEntity:
          Name:
            NameFull: Scherzad, Agmal
      – PersonEntity:
          Name:
            NameFull: Delaval, Mathilde Noémie
      – PersonEntity:
          Name:
            NameFull: Zimmermann, Ralf
      – PersonEntity:
          Name:
            NameFull: Di Bucchianico, Sebastiano
      – PersonEntity:
          Name:
            NameFull: Hackenberg, Stephan
      – PersonEntity:
          Name:
            NameFull: Meyer, Till J.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 10
              Text: Oct2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 13826689
          Numbering:
            – Type: volume
              Value: 119
          Titles:
            – TitleFull: Environmental Toxicology & Pharmacology
              Type: main
ResultId 1