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The AMPK-FOXO signaling regulates the transcriptional response of 4EBP to pesticide stress in Tribolium castaneum.

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Title: The AMPK-FOXO signaling regulates the transcriptional response of 4EBP to pesticide stress in Tribolium castaneum.
Authors: Guan, Daojie1 (AUTHOR), Wang, Zhichao1 (AUTHOR), Liu, Changpeng1 (AUTHOR), Ge, Huichen1 (AUTHOR), Zhou, Mi1 (AUTHOR), Fang, Shu1 (AUTHOR), Qian, Kun1 (AUTHOR), Wang, Jianjun1,2 (AUTHOR) wangjj@yzu.edu.cn
Source: Pesticide Biochemistry & Physiology. Jan2026:Part 1, Vol. 216, pN.PAG-N.PAG. 1p.
Subject Terms: *Pesticide pollution, Red flour beetle, Deltamethrin, Genetic transcription regulation, Protein synthesis, AMP-activated protein kinases, Forkhead transcription factors, RNA interference
Abstract: The eukaryotic initiation factor 4E-binding protein (4EBP) regulates protein synthesis by suppressing cap-dependent translation, the most energy-consuming process in the cell. While transcriptional and post-translational modulation of 4EBP has been extensively studied in mammals, its roles in response to pesticide stress in insects remain poorly understood. In this study, we identified and cloned the Tc4EBP gene from Tribolium castaneum. RT-qPCR analysis revealed that the expression level of Tc4EBP increased with the development time during larval, pupal and adult stages. RNA interference (RNAi) experiment showed that silencing Tc4EBP through dsRNA injection decreased pupation and eclosion rates of the beetles. Additionally, the mRNA level of Tc4EBP was significantly induced by deltamethrin treatment, and knockdown of Tc4EBP significantly increased susceptibility of the beetles to deltamethrin and enhanced deltamethrin-induced ATP depletion. Notably, knockdown of TcFOXO and TcAMPKα significantly downregulated the expression level of Tc4EBP and attenuated its induction by deltamethrin. Furthermore, knockdown of TcFOXO prevented the induction of Tc4EBP by treatment with the AMPK activator AICAR. This study establishes Tc4EBP is involved in development of T. castaneum , and TcAMPK-TcFOXO signaling positively regulated Tc4EBP transcription in response to deltamethrin treatment, providing evidence of a survival strategy under pesticide stress via inhibition of translation. [Display omitted] • Knockdown of Tc4EBP negatively affected pupation and eclosion in T. castaneum. • Tc4EBP was upregulated in beetles after exposure to deltamethrin. • Knockdown of Tc4EBP decreased tolerance of beetles to deltamethrin. • AMPK-FOXO signaling mediated deltamethrin-induced expression of Tc4EBP. [ABSTRACT FROM AUTHOR]
Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: The AMPK-FOXO signaling regulates the transcriptional response of 4EBP to pesticide stress in Tribolium castaneum.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Guan%2C+Daojie%22">Guan, Daojie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhichao%22">Wang, Zhichao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Changpeng%22">Liu, Changpeng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ge%2C+Huichen%22">Ge, Huichen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Mi%22">Zhou, Mi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fang%2C+Shu%22">Fang, Shu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qian%2C+Kun%22">Qian, Kun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Jianjun%22">Wang, Jianjun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> wangjj@yzu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Pesticide+Biochemistry+%26+Physiology%22">Pesticide Biochemistry & Physiology</searchLink>. Jan2026:Part 1, Vol. 216, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Pesticide+pollution%22">Pesticide pollution</searchLink><br /><searchLink fieldCode="DE" term="%22Red+flour+beetle%22">Red flour beetle</searchLink><br /><searchLink fieldCode="DE" term="%22Deltamethrin%22">Deltamethrin</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+transcription+regulation%22">Genetic transcription regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+synthesis%22">Protein synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22AMP-activated+protein+kinases%22">AMP-activated protein kinases</searchLink><br /><searchLink fieldCode="DE" term="%22Forkhead+transcription+factors%22">Forkhead transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+interference%22">RNA interference</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The eukaryotic initiation factor 4E-binding protein (4EBP) regulates protein synthesis by suppressing cap-dependent translation, the most energy-consuming process in the cell. While transcriptional and post-translational modulation of 4EBP has been extensively studied in mammals, its roles in response to pesticide stress in insects remain poorly understood. In this study, we identified and cloned the Tc4EBP gene from Tribolium castaneum. RT-qPCR analysis revealed that the expression level of Tc4EBP increased with the development time during larval, pupal and adult stages. RNA interference (RNAi) experiment showed that silencing Tc4EBP through dsRNA injection decreased pupation and eclosion rates of the beetles. Additionally, the mRNA level of Tc4EBP was significantly induced by deltamethrin treatment, and knockdown of Tc4EBP significantly increased susceptibility of the beetles to deltamethrin and enhanced deltamethrin-induced ATP depletion. Notably, knockdown of TcFOXO and TcAMPKα significantly downregulated the expression level of Tc4EBP and attenuated its induction by deltamethrin. Furthermore, knockdown of TcFOXO prevented the induction of Tc4EBP by treatment with the AMPK activator AICAR. This study establishes Tc4EBP is involved in development of T. castaneum , and TcAMPK-TcFOXO signaling positively regulated Tc4EBP transcription in response to deltamethrin treatment, providing evidence of a survival strategy under pesticide stress via inhibition of translation. [Display omitted] • Knockdown of Tc4EBP negatively affected pupation and eclosion in T. castaneum. • Tc4EBP was upregulated in beetles after exposure to deltamethrin. • Knockdown of Tc4EBP decreased tolerance of beetles to deltamethrin. • AMPK-FOXO signaling mediated deltamethrin-induced expression of Tc4EBP. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Pesticide Biochemistry & Physiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1016/j.pestbp.2025.106724
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Pesticide pollution
        Type: general
      – SubjectFull: Red flour beetle
        Type: general
      – SubjectFull: Deltamethrin
        Type: general
      – SubjectFull: Genetic transcription regulation
        Type: general
      – SubjectFull: Protein synthesis
        Type: general
      – SubjectFull: AMP-activated protein kinases
        Type: general
      – SubjectFull: Forkhead transcription factors
        Type: general
      – SubjectFull: RNA interference
        Type: general
    Titles:
      – TitleFull: The AMPK-FOXO signaling regulates the transcriptional response of 4EBP to pesticide stress in Tribolium castaneum.
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            NameFull: Guan, Daojie
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            NameFull: Wang, Zhichao
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            NameFull: Liu, Changpeng
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            – D: 01
              M: 01
              Text: Jan2026:Part 1
              Type: published
              Y: 2026
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            – Type: issn-print
              Value: 00483575
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              Value: 216
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            – TitleFull: Pesticide Biochemistry & Physiology
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