Carbendazim detection in human follicular fluid: Impact on human granulosa cell function (KGNs and primary cells) and mouse ovarian physiology.
Saved in:
| Title: | Carbendazim detection in human follicular fluid: Impact on human granulosa cell function (KGNs and primary cells) and mouse ovarian physiology. |
|---|---|
| Authors: | Berthet, Lucille1 (AUTHOR) lucille.berthet@inrae.fr, Rame, Christelle1 (AUTHOR) christelle.rame@inrae.fr, Le lagadec, Marie1 (AUTHOR) marie.le-lagadec@inrae.fr, Chevaleyre, Claire1 (AUTHOR) claire.chevaleyre@inrae.fr, Couty, Noemie1 (AUTHOR) noemie.couty@inrae.fr, Corbin, Emilie1 (AUTHOR) emilie.corbin@inrae.fr, Amalric, Laurence2 (AUTHOR) l.amalric@brgm.fr, Coureau, Charlotte2 (AUTHOR) c.coureau@brgm.fr, Coisy, Emeline2 (AUTHOR) e.coisy@brgm.fr, Guérif, Fabrice1,3 (AUTHOR) fabrice.guerif@univ-tours.fr, Froment, Pascal1 (AUTHOR) pascal.froment@inrae.fr, Dupont, Joelle1 (AUTHOR) joelle.dupont@inrae.fr |
| Source: | Environmental Toxicology & Pharmacology. Aug2026, Vol. 125, pN.PAG-N.PAG. 1p. |
| Subject Terms: | *Endocrine disruptors, Carbendazim, Granulosa cells, Steroid synthesis, Oxidative stress, Ovaries, Ovarian follicle, Reproductive toxicology |
| Abstract: | This study investigates the endocrine-disrupting effects of the fungicide carbendazim (CBZ) on human and murine reproductive health. Mass spectrometry confirmed systemic human exposure, detecting CBZ in the plasma and follicular fluid of 15–18% of the women sampled. In vitro, CBZ did not affect viability in human KGN and primary granulosa cells (hGCs) but significantly inhibited KGN proliferation. Furthermore, CBZ reduced steroidogenesis stimulated by FSH, LH, or IGF-1 in both cell types. This dysfunction correlated with a dose-dependent increase in the Oxidative Stress Index, driven by elevated total oxidant status and reactive oxygen species. Corroborating these findings, in vivo murine models exposed to CBZ (100 μg/kg/day) exhibited a prolonged diestrus phase, alongside reduced numbers of corpora lutea and preovulatory follicles, and decreased gonadotropin and E2 plasma levels. Collectively, these results identify CBZ as a potent reproductive toxicant that impairs ovarian function via oxidative stress-mediated hormonal disruption. • Carbendazim (CBZ) is detected in human follicular fluid as well as in blood plasma • CBZ increases oxidative stress in human granulosa cells • CBZ inhibits FSH, LH, and IGF1-stimulated steroid secretion in human granulosa cells • CBZ oral exposure in mice leads to decreased gonadotropin and E2 plasma levels • CBZ oral exposure in mice leads to disrupted estrus cycles and altered folliculogenesis. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Toxicology & Pharmacology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | GreenFILE |
Be the first to leave a comment!
