View in EDS

Enhancer elements in the mouse CYP1A2 gene: A comparative sequencing among different inbred mouse strains

Saved in:
Bibliographic Details
Title: Enhancer elements in the mouse CYP1A2 gene: A comparative sequencing among different inbred mouse strains
Authors: Mikhailova, Olga N.1,2 pharmacogenomics@ngs.ru, Gulyaeva, Lyudmila F.1, Filipenko, Maxim L.2, Kaledin, Vasily I.3
Source: Mutation Research - Genetic Toxicology & Environmental Mutagenesis. Aug2007, Vol. 632 Issue 1/2, p99-103. 5p.
Subject Terms: Cytochrome P-450, Genetic polymorphism research, Laboratory mice, Medical research
Abstract: Abstract: CYP1A2 expression is constitutively high in mouse liver and is well known for metabolizing several drugs and many procarcinogens to reactive intermediates that can cause toxicity or cancer. In the present study, the basal level of hepatic CYP1A2 activity was shown to vary among different inbred mouse strains. The highest methoxyresorufin-O-demethylase activity (261±52pmol/mgprotein/min) was registered in CC57BR and the lowest (82±11pmol/mgprotein/min) in C3H/a. We have tested the hypothesis that possible polymorphisms in regulatory elements in the 5′-upstream region of the mouse CYP1A2 gene could cause the differences in CYP1A2 enzyme activity among different inbred strains. We have performed a study on the CYP1A2 gene by sequencing the regulatory region from −4675 to −4204 where two enhancer elements were recently identified. The absence of mutation prescribing the phenotype in the CYP1A2 gene was found. The region studied seems to be a highly conserved in mice and not to be associated with interstrain differences in constitutive CYP1A2 enzyme activity. [Copyright &y& Elsevier]
Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: GreenFILE
FullText Text:
  Availability: 0
Header DbId: 8gh
DbLabel: GreenFILE
An: 25751274
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Enhancer elements in the mouse CYP1A2 gene: A comparative sequencing among different inbred mouse strains
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Mikhailova%2C+Olga+N%2E%22">Mikhailova, Olga N.</searchLink><relatesTo>1,2</relatesTo><i> pharmacogenomics@ngs.ru</i><br /><searchLink fieldCode="AR" term="%22Gulyaeva%2C+Lyudmila+F%2E%22">Gulyaeva, Lyudmila F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Filipenko%2C+Maxim+L%2E%22">Filipenko, Maxim L.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kaledin%2C+Vasily+I%2E%22">Kaledin, Vasily I.</searchLink><relatesTo>3</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Mutation+Research+-+Genetic+Toxicology+%26+Environmental+Mutagenesis%22">Mutation Research - Genetic Toxicology & Environmental Mutagenesis</searchLink>. Aug2007, Vol. 632 Issue 1/2, p99-103. 5p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Cytochrome+P-450%22">Cytochrome P-450</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+polymorphism+research%22">Genetic polymorphism research</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+mice%22">Laboratory mice</searchLink><br /><searchLink fieldCode="DE" term="%22Medical+research%22">Medical research</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: CYP1A2 expression is constitutively high in mouse liver and is well known for metabolizing several drugs and many procarcinogens to reactive intermediates that can cause toxicity or cancer. In the present study, the basal level of hepatic CYP1A2 activity was shown to vary among different inbred mouse strains. The highest methoxyresorufin-O-demethylase activity (261±52pmol/mgprotein/min) was registered in CC57BR and the lowest (82±11pmol/mgprotein/min) in C3H/a. We have tested the hypothesis that possible polymorphisms in regulatory elements in the 5′-upstream region of the mouse CYP1A2 gene could cause the differences in CYP1A2 enzyme activity among different inbred strains. We have performed a study on the CYP1A2 gene by sequencing the regulatory region from −4675 to −4204 where two enhancer elements were recently identified. The absence of mutation prescribing the phenotype in the CYP1A2 gene was found. The region studied seems to be a highly conserved in mice and not to be associated with interstrain differences in constitutive CYP1A2 enzyme activity. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=8gh&AN=25751274
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.mrgentox.2007.04.013
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 5
        StartPage: 99
    Subjects:
      – SubjectFull: Cytochrome P-450
        Type: general
      – SubjectFull: Genetic polymorphism research
        Type: general
      – SubjectFull: Laboratory mice
        Type: general
      – SubjectFull: Medical research
        Type: general
    Titles:
      – TitleFull: Enhancer elements in the mouse CYP1A2 gene: A comparative sequencing among different inbred mouse strains
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Mikhailova, Olga N.
      – PersonEntity:
          Name:
            NameFull: Gulyaeva, Lyudmila F.
      – PersonEntity:
          Name:
            NameFull: Filipenko, Maxim L.
      – PersonEntity:
          Name:
            NameFull: Kaledin, Vasily I.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 15
              M: 08
              Text: Aug2007
              Type: published
              Y: 2007
          Identifiers:
            – Type: issn-print
              Value: 13835718
          Numbering:
            – Type: volume
              Value: 632
            – Type: issue
              Value: 1/2
          Titles:
            – TitleFull: Mutation Research - Genetic Toxicology & Environmental Mutagenesis
              Type: main
ResultId 1