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Continuous exposure to bisphenol A during in vitro follicular development induces meiotic abnormalities

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Title: Continuous exposure to bisphenol A during in vitro follicular development induces meiotic abnormalities
Authors: Lenie, Sandy1 Sandy.Lenie@vub.ac.be, Cortvrindt, Rita1,2, Eichenlaub-Ritter, Ursula3, Smitz, Johan1
Source: Mutation Research - Genetic Toxicology & Environmental Mutagenesis. Mar2008, Vol. 651 Issue 1/2, p71-81. 11p.
Subject Terms: *Genetics, Bisphenol A, Meiosis, Genetic mutation
Abstract: Abstract: Bisphenol A (BPA), a widely used environmental contaminant, may exert weak estrogenic, anti-androgenic and anti-thyroidic activities. BPA is suspected to possess aneugenic properties that may affect somatic cells and mammalian oocytes. Oocyte growth and maturation depend upon a complex bi-directional signaling between the oocyte and its companion somatic cells. Consequently, disturbances in oocyte maturation may originate either from direct effects of BPA at the level of the oocyte or from indirect influences at the follicular level, such as alterations in hormonal homeostasis. This study aimed to analyze the effects of chronic BPA exposure (3nM to 30μM) on follicle-enclosed growth and maturation of mouse oocytes in vitro. Oocytes were cultured and their spindle and chromosomes were stained by α-tubulin immunofluorescence and ethidium homodimer-2, respectively. Confocal microscopy was utilized for subsequent analysis. Only follicles that were exposed to 30μM BPA during follicular development showed a slightly reduced granulosa cell proliferation and a lower total estrogen production, but they still developed and formed antral-like cavities. However, 18% of oocytes were unable to resume meiosis after stimulation of oocyte maturation, and 37% arrested after germinal vesicle breakdown, significantly different from controls (p <0.05). Only 45% of the oocytes extruded a first polar body (p <0.05). 30μM BPA led also to a significant increase in meiosis I-arrested oocytes with unaligned chromosomes and spindle aberrations. Oocytes that were able to progress beyond meiosis I, frequently arrested at an abnormal telophase I. Additionally, in many oocytes exposed to low chronic BPA that matured to meiosis II chromosomes failed to congress at the spindle equator. In conclusion, mouse follicle culture reveals non-linear dose-dependent effects of BPA on the meiotic spindle in mouse oocytes when exposure was chronic throughout oocyte growth and maturation. [Copyright &y& Elsevier]
Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Continuous exposure to bisphenol A during in vitro follicular development induces meiotic abnormalities
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Lenie%2C+Sandy%22&quot;&gt;Lenie, Sandy&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;i&gt; Sandy.Lenie@vub.ac.be&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cortvrindt%2C+Rita%22&quot;&gt;Cortvrindt, Rita&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Eichenlaub-Ritter%2C+Ursula%22&quot;&gt;Eichenlaub-Ritter, Ursula&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Smitz%2C+Johan%22&quot;&gt;Smitz, Johan&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Mutation+Research+-+Genetic+Toxicology+%26+Environmental+Mutagenesis%22&quot;&gt;Mutation Research - Genetic Toxicology &amp; Environmental Mutagenesis&lt;/searchLink&gt;. Mar2008, Vol. 651 Issue 1/2, p71-81. 11p.
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  Data: *&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Genetics%22&quot;&gt;Genetics&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Bisphenol+A%22&quot;&gt;Bisphenol A&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Meiosis%22&quot;&gt;Meiosis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Genetic+mutation%22&quot;&gt;Genetic mutation&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
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  Data: Abstract: Bisphenol A (BPA), a widely used environmental contaminant, may exert weak estrogenic, anti-androgenic and anti-thyroidic activities. BPA is suspected to possess aneugenic properties that may affect somatic cells and mammalian oocytes. Oocyte growth and maturation depend upon a complex bi-directional signaling between the oocyte and its companion somatic cells. Consequently, disturbances in oocyte maturation may originate either from direct effects of BPA at the level of the oocyte or from indirect influences at the follicular level, such as alterations in hormonal homeostasis. This study aimed to analyze the effects of chronic BPA exposure (3nM to 30μM) on follicle-enclosed growth and maturation of mouse oocytes in vitro. Oocytes were cultured and their spindle and chromosomes were stained by α-tubulin immunofluorescence and ethidium homodimer-2, respectively. Confocal microscopy was utilized for subsequent analysis. Only follicles that were exposed to 30μM BPA during follicular development showed a slightly reduced granulosa cell proliferation and a lower total estrogen production, but they still developed and formed antral-like cavities. However, 18% of oocytes were unable to resume meiosis after stimulation of oocyte maturation, and 37% arrested after germinal vesicle breakdown, significantly different from controls (p &lt;0.05). Only 45% of the oocytes extruded a first polar body (p &lt;0.05). 30μM BPA led also to a significant increase in meiosis I-arrested oocytes with unaligned chromosomes and spindle aberrations. Oocytes that were able to progress beyond meiosis I, frequently arrested at an abnormal telophase I. Additionally, in many oocytes exposed to low chronic BPA that matured to meiosis II chromosomes failed to congress at the spindle equator. In conclusion, mouse follicle culture reveals non-linear dose-dependent effects of BPA on the meiotic spindle in mouse oocytes when exposure was chronic throughout oocyte growth and maturation. [Copyright &amp;y&amp; Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: &lt;i&gt;Copyright of Mutation Research - Genetic Toxicology &amp; Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.mrgentox.2007.10.017
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      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 71
    Subjects:
      – SubjectFull: Genetics
        Type: general
      – SubjectFull: Bisphenol A
        Type: general
      – SubjectFull: Meiosis
        Type: general
      – SubjectFull: Genetic mutation
        Type: general
    Titles:
      – TitleFull: Continuous exposure to bisphenol A during in vitro follicular development induces meiotic abnormalities
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Lenie, Sandy
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            NameFull: Cortvrindt, Rita
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            NameFull: Eichenlaub-Ritter, Ursula
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            NameFull: Smitz, Johan
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            – D: 12
              M: 03
              Text: Mar2008
              Type: published
              Y: 2008
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              Value: 651
            – Type: issue
              Value: 1/2
          Titles:
            – TitleFull: Mutation Research - Genetic Toxicology & Environmental Mutagenesis
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