View in EDS

Trichlorfon-induced polyploidy and nondisjunction in mouse oocytes from preantral follicle culture

Saved in:
Bibliographic Details
Title: Trichlorfon-induced polyploidy and nondisjunction in mouse oocytes from preantral follicle culture
Authors: Sun, Fengyun1, Betzendahl, Ilse1, Van Wemmel, Kelly2, Cortvrindt, Rita2, Smitz, Johan2, Pacchierotti, Francesca3, Eichenlaub-Ritter, Ursula1 EIRI@uni-bielefeld.de
Source: Mutation Research - Genetic Toxicology & Environmental Mutagenesis. Mar2008, Vol. 651 Issue 1/2, p114-124. 11p.
Subject Terms: Polyploidy, Ovum, Laboratory mice, Genetic mutation
Abstract: Abstract: Trichlorfon (TCF), an organophosphate insecticide and potent inhibitor of choline esterases, was previously shown to induce first meiotic nondisjunction and spindle aberrations in isolated, follicle cell-denuded mouse oocytes maturing in vitro. To explore dose-response and direct and indirect, potentially synergistic effects of TCF on the somatic cells and the oocyte within a follicle, we presently employed preantral follicle culture. 100μg/ml TCF added at the time of hormonally stimulated resumption of meiosis of follicle cell-enclosed mouse oocytes, 16h before in vitro ovulation, induced significant rises in first meiotic nondisjunction in oocytes from preantral follicle culture. Lower concentrations (6μg/ml TCF) disturbed polar body formation. Nuclear maturation to meiosis II in absence of cytokinesis resulted in significant increases in polyploidy. Oocytes maturing in follicles in the presence of TCF had aberrant second meiotic spindles. Influences of TCF on somatic cell function were evident by reduced follicular mucification in vitro and deceased progesterone production. In contrast to TCF, acetylcholine (0.1–100μM) increased progesterone production. The observations therefore suggest that TCF influences oocyte maturation and folliculogenesis directly and indirectly. High TCF is aneugenic at first meiotic division in oocytes, irrespective of the presence or absence of follicle cells. At lower concentrations TCF interferes with spindle formation, chromosome congression at meiosis II, and coordination of nuclear and cytoplasmic maturation, posing risks for second meiotic errors. The observations suggest that chronic TCF exposure during maturation in the follicle may predispose oocytes to the formation of chromosomally unbalanced preimplantation embryos after fertilization. [Copyright &y& Elsevier]
Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: GreenFILE
FullText Text:
  Availability: 0
Header DbId: 8gh
DbLabel: GreenFILE
An: 30874476
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Trichlorfon-induced polyploidy and nondisjunction in mouse oocytes from preantral follicle culture
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Sun%2C+Fengyun%22">Sun, Fengyun</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Betzendahl%2C+Ilse%22">Betzendahl, Ilse</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Van+Wemmel%2C+Kelly%22">Van Wemmel, Kelly</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Cortvrindt%2C+Rita%22">Cortvrindt, Rita</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Smitz%2C+Johan%22">Smitz, Johan</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Pacchierotti%2C+Francesca%22">Pacchierotti, Francesca</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Eichenlaub-Ritter%2C+Ursula%22">Eichenlaub-Ritter, Ursula</searchLink><relatesTo>1</relatesTo><i> EIRI@uni-bielefeld.de</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Mutation+Research+-+Genetic+Toxicology+%26+Environmental+Mutagenesis%22">Mutation Research - Genetic Toxicology & Environmental Mutagenesis</searchLink>. Mar2008, Vol. 651 Issue 1/2, p114-124. 11p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Polyploidy%22">Polyploidy</searchLink><br /><searchLink fieldCode="DE" term="%22Ovum%22">Ovum</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+mice%22">Laboratory mice</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+mutation%22">Genetic mutation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Trichlorfon (TCF), an organophosphate insecticide and potent inhibitor of choline esterases, was previously shown to induce first meiotic nondisjunction and spindle aberrations in isolated, follicle cell-denuded mouse oocytes maturing in vitro. To explore dose-response and direct and indirect, potentially synergistic effects of TCF on the somatic cells and the oocyte within a follicle, we presently employed preantral follicle culture. 100μg/ml TCF added at the time of hormonally stimulated resumption of meiosis of follicle cell-enclosed mouse oocytes, 16h before in vitro ovulation, induced significant rises in first meiotic nondisjunction in oocytes from preantral follicle culture. Lower concentrations (6μg/ml TCF) disturbed polar body formation. Nuclear maturation to meiosis II in absence of cytokinesis resulted in significant increases in polyploidy. Oocytes maturing in follicles in the presence of TCF had aberrant second meiotic spindles. Influences of TCF on somatic cell function were evident by reduced follicular mucification in vitro and deceased progesterone production. In contrast to TCF, acetylcholine (0.1–100μM) increased progesterone production. The observations therefore suggest that TCF influences oocyte maturation and folliculogenesis directly and indirectly. High TCF is aneugenic at first meiotic division in oocytes, irrespective of the presence or absence of follicle cells. At lower concentrations TCF interferes with spindle formation, chromosome congression at meiosis II, and coordination of nuclear and cytoplasmic maturation, posing risks for second meiotic errors. The observations suggest that chronic TCF exposure during maturation in the follicle may predispose oocytes to the formation of chromosomally unbalanced preimplantation embryos after fertilization. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Mutation Research - Genetic Toxicology & Environmental Mutagenesis is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=8gh&AN=30874476
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.mrgentox.2007.10.008
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 114
    Subjects:
      – SubjectFull: Polyploidy
        Type: general
      – SubjectFull: Ovum
        Type: general
      – SubjectFull: Laboratory mice
        Type: general
      – SubjectFull: Genetic mutation
        Type: general
    Titles:
      – TitleFull: Trichlorfon-induced polyploidy and nondisjunction in mouse oocytes from preantral follicle culture
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Sun, Fengyun
      – PersonEntity:
          Name:
            NameFull: Betzendahl, Ilse
      – PersonEntity:
          Name:
            NameFull: Van Wemmel, Kelly
      – PersonEntity:
          Name:
            NameFull: Cortvrindt, Rita
      – PersonEntity:
          Name:
            NameFull: Smitz, Johan
      – PersonEntity:
          Name:
            NameFull: Pacchierotti, Francesca
      – PersonEntity:
          Name:
            NameFull: Eichenlaub-Ritter, Ursula
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 12
              M: 03
              Text: Mar2008
              Type: published
              Y: 2008
          Identifiers:
            – Type: issn-print
              Value: 13835718
          Numbering:
            – Type: volume
              Value: 651
            – Type: issue
              Value: 1/2
          Titles:
            – TitleFull: Mutation Research - Genetic Toxicology & Environmental Mutagenesis
              Type: main
ResultId 1