Aptamer-based piezoelectric quartz crystal microbalance biosensor array for the quantification of IgE
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| Title: | Aptamer-based piezoelectric quartz crystal microbalance biosensor array for the quantification of IgE |
|---|---|
| Authors: | Yao, Chunyan1 yao_yao24@yahoo.com, Qi, Yongzhi1, Zhao, Yuhui1, Xiang, Yang1, Chen, Qinghai1, Fu, Weiling weilingfu@yahoo.com |
| Source: | Biosensors & Bioelectronics. Apr2009, Vol. 24 Issue 8, p2499-2503. 5p. |
| Subject Terms: | Biosensors, Blood testing, Serum, Immunoglobulin E, Quartz crystal microbalances, Peptides, Bioelectronics |
| Abstract: | Abstract: The aim of this study was to develop a rapid method to measure IgE in human serum by use of a direct aptamer-based biosensor based on a quartz crystal microbalance (QCM). An avidin monolayer was applied to immobilize aptamers specific for IgE on the gold surface of a quartz crystal. The frequency shifts (FS) of the QCM were measured and related to IgE concentrations. We could demonstrate that aptamers were able to detect IgE with high specificity and sensitivity in 15min. A linear relationship existed between the FS (Hz) and the IgE concentrations from 2.5 to 200μg/L in buffer and human serum. The regression equation was y =1.03x −0.06 for this QCM method and chemiluminescence in 50 clinical human serum samples. In addition, the aptamer receptors tolerated repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for 3 weeks, the FS were all greater than 90% of those on the response at the first day. The QCM biosensor can measure IgE and offer advantages of high specificity, reusability, low detection limit, no label or sample pretreatment, and low sample requirement. The aptamer QCM biosensor was suitable for sensitive and specific protein detection, representing an innovative tool for future proteomics. [Copyright &y& Elsevier] |
| Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | GreenFILE |
| FullText | Text: Availability: 0 |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 37228194 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Aptamer-based piezoelectric quartz crystal microbalance biosensor array for the quantification of IgE – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Yao%2C+Chunyan%22">Yao, Chunyan</searchLink><relatesTo>1</relatesTo><i> yao_yao24@yahoo.com</i><br /><searchLink fieldCode="AR" term="%22Qi%2C+Yongzhi%22">Qi, Yongzhi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhao%2C+Yuhui%22">Zhao, Yuhui</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xiang%2C+Yang%22">Xiang, Yang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Qinghai%22">Chen, Qinghai</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fu%2C+Weiling%22">Fu, Weiling</searchLink><i> weilingfu@yahoo.com</i> – Name: TitleSource Label: Source Group: Src Data: Biosensors & Bioelectronics. Apr2009, Vol. 24 Issue 8, p2499-2503. 5p. – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+testing%22">Blood testing</searchLink><br /><searchLink fieldCode="DE" term="%22Serum%22">Serum</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoglobulin+E%22">Immunoglobulin E</searchLink><br /><searchLink fieldCode="DE" term="%22Quartz+crystal+microbalances%22">Quartz crystal microbalances</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Bioelectronics%22">Bioelectronics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: The aim of this study was to develop a rapid method to measure IgE in human serum by use of a direct aptamer-based biosensor based on a quartz crystal microbalance (QCM). An avidin monolayer was applied to immobilize aptamers specific for IgE on the gold surface of a quartz crystal. The frequency shifts (FS) of the QCM were measured and related to IgE concentrations. We could demonstrate that aptamers were able to detect IgE with high specificity and sensitivity in 15min. A linear relationship existed between the FS (Hz) and the IgE concentrations from 2.5 to 200μg/L in buffer and human serum. The regression equation was y =1.03x −0.06 for this QCM method and chemiluminescence in 50 clinical human serum samples. In addition, the aptamer receptors tolerated repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for 3 weeks, the FS were all greater than 90% of those on the response at the first day. The QCM biosensor can measure IgE and offer advantages of high specificity, reusability, low detection limit, no label or sample pretreatment, and low sample requirement. The aptamer QCM biosensor was suitable for sensitive and specific protein detection, representing an innovative tool for future proteomics. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.bios.2008.12.036 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 2499 Subjects: – SubjectFull: Biosensors Type: general – SubjectFull: Blood testing Type: general – SubjectFull: Serum Type: general – SubjectFull: Immunoglobulin E Type: general – SubjectFull: Quartz crystal microbalances Type: general – SubjectFull: Peptides Type: general – SubjectFull: Bioelectronics Type: general Titles: – TitleFull: Aptamer-based piezoelectric quartz crystal microbalance biosensor array for the quantification of IgE Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Yao, Chunyan – PersonEntity: Name: NameFull: Qi, Yongzhi – PersonEntity: Name: NameFull: Zhao, Yuhui – PersonEntity: Name: NameFull: Xiang, Yang – PersonEntity: Name: NameFull: Chen, Qinghai – PersonEntity: Name: NameFull: Fu, Weiling IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 04 Text: Apr2009 Type: published Y: 2009 Identifiers: – Type: issn-print Value: 09565663 Numbering: – Type: volume Value: 24 – Type: issue Value: 8 Titles: – TitleFull: Biosensors & Bioelectronics Type: main |
| ResultId | 1 |
