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Quercetin protects embryonic chicken spermatogonial cells from oxidative damage intoxicated with 3-methyl-4-nitrophenol in primary culture

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Title: Quercetin protects embryonic chicken spermatogonial cells from oxidative damage intoxicated with 3-methyl-4-nitrophenol in primary culture
Authors: Mi, Yuling1,2, Zhang, Caiqiao2 cqzhang@zju.edu.cn, Li, ChunMei3,4, Taneda, Shinji3, Watanabe, Gen1,5, Suzuki, Akira K.3, Taya, Kazuyoshi1,5
Source: Toxicology Letters. Oct2009, Vol. 190 Issue 1, p61-65. 5p.
Subject Terms: *Air pollution, *Diesel motor exhaust gas, Quercetin, Chickens, Spermatogenesis, Oxidative stress, Nitrophenols, Antioxidants, Cytoplasm, Superoxide dismutase, Therapeutics
Abstract: Abstract: Diesel exhaust particles (DEP) are considered to be one of the most important air pollutants. In this study, the protective effect of quercetin, an antioxidant flavonoid, on oxidative damage of testicular cells was studied by analysis of the intracellular antioxidant system of embryonic chickens after treatment with 3-methyl-4-nitrophenol (PNMC) derived from DEP. Testicular cells from 18-day-old embryos were cultured in serum-free McCoys’5A medium and challenged with PNMC (10−7 to 10−5 M) alone or in combinations with quercetin (1.0μg/ml) for 48h. Results showed that exposure to PNMC (10−5 M) induced condensed nuclei and vacuolated cytoplasm, a decrease in testicular cell viability and spermatogonial cell number. Exposure to PNMC induced lipid peroxidation by an elevation of thiobarbituric acid reactive substances as well as decreasing glutathione peroxidation activity and superoxide dismutase activity. However, simultaneous supplementation with quercetin restored these parameters to the similar levels as the control. PNMC is therefore concluded to have induced the oxidative stress of the spermatogonial cells, which can be attenuated by combined quercetin treatment. Our results support the therapeutic use of quercetin in the prevention or treatment of the reproductive toxicity by environmental toxicant PNMC. [Copyright &y& Elsevier]
Copyright of Toxicology Letters is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Quercetin protects embryonic chicken spermatogonial cells from oxidative damage intoxicated with 3-methyl-4-nitrophenol in primary culture
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  Data: <searchLink fieldCode="AR" term="%22Mi%2C+Yuling%22">Mi, Yuling</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Caiqiao%22">Zhang, Caiqiao</searchLink><relatesTo>2</relatesTo><i> cqzhang@zju.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Li%2C+ChunMei%22">Li, ChunMei</searchLink><relatesTo>3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Taneda%2C+Shinji%22">Taneda, Shinji</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Watanabe%2C+Gen%22">Watanabe, Gen</searchLink><relatesTo>1,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Suzuki%2C+Akira+K%2E%22">Suzuki, Akira K.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Taya%2C+Kazuyoshi%22">Taya, Kazuyoshi</searchLink><relatesTo>1,5</relatesTo>
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  Data: Toxicology Letters. Oct2009, Vol. 190 Issue 1, p61-65. 5p.
– Name: Subject
  Label: Subject Terms
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  Data: *<searchLink fieldCode="DE" term="%22Air+pollution%22">Air pollution</searchLink><br />*<searchLink fieldCode="DE" term="%22Diesel+motor+exhaust+gas%22">Diesel motor exhaust gas</searchLink><br /><searchLink fieldCode="DE" term="%22Quercetin%22">Quercetin</searchLink><br /><searchLink fieldCode="DE" term="%22Chickens%22">Chickens</searchLink><br /><searchLink fieldCode="DE" term="%22Spermatogenesis%22">Spermatogenesis</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidative+stress%22">Oxidative stress</searchLink><br /><searchLink fieldCode="DE" term="%22Nitrophenols%22">Nitrophenols</searchLink><br /><searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Cytoplasm%22">Cytoplasm</searchLink><br /><searchLink fieldCode="DE" term="%22Superoxide+dismutase%22">Superoxide dismutase</searchLink><br /><searchLink fieldCode="DE" term="%22Therapeutics%22">Therapeutics</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Diesel exhaust particles (DEP) are considered to be one of the most important air pollutants. In this study, the protective effect of quercetin, an antioxidant flavonoid, on oxidative damage of testicular cells was studied by analysis of the intracellular antioxidant system of embryonic chickens after treatment with 3-methyl-4-nitrophenol (PNMC) derived from DEP. Testicular cells from 18-day-old embryos were cultured in serum-free McCoys’5A medium and challenged with PNMC (10−7 to 10−5 M) alone or in combinations with quercetin (1.0μg/ml) for 48h. Results showed that exposure to PNMC (10−5 M) induced condensed nuclei and vacuolated cytoplasm, a decrease in testicular cell viability and spermatogonial cell number. Exposure to PNMC induced lipid peroxidation by an elevation of thiobarbituric acid reactive substances as well as decreasing glutathione peroxidation activity and superoxide dismutase activity. However, simultaneous supplementation with quercetin restored these parameters to the similar levels as the control. PNMC is therefore concluded to have induced the oxidative stress of the spermatogonial cells, which can be attenuated by combined quercetin treatment. Our results support the therapeutic use of quercetin in the prevention or treatment of the reproductive toxicity by environmental toxicant PNMC. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Toxicology Letters is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
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      – Type: doi
        Value: 10.1016/j.toxlet.2009.07.002
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 5
        StartPage: 61
    Subjects:
      – SubjectFull: Air pollution
        Type: general
      – SubjectFull: Diesel motor exhaust gas
        Type: general
      – SubjectFull: Quercetin
        Type: general
      – SubjectFull: Chickens
        Type: general
      – SubjectFull: Spermatogenesis
        Type: general
      – SubjectFull: Oxidative stress
        Type: general
      – SubjectFull: Nitrophenols
        Type: general
      – SubjectFull: Antioxidants
        Type: general
      – SubjectFull: Cytoplasm
        Type: general
      – SubjectFull: Superoxide dismutase
        Type: general
      – SubjectFull: Therapeutics
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      – TitleFull: Quercetin protects embryonic chicken spermatogonial cells from oxidative damage intoxicated with 3-methyl-4-nitrophenol in primary culture
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            NameFull: Mi, Yuling
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            NameFull: Zhang, Caiqiao
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            – D: 08
              M: 10
              Text: Oct2009
              Type: published
              Y: 2009
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              Value: 03784274
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