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GENOTOXIC EFFECTS OF LOW DOSES OF PESTICIDES ON MONONUCLEAR LEUKOCYTE ISOLATED FROM HIGHER CELLS.

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Title: GENOTOXIC EFFECTS OF LOW DOSES OF PESTICIDES ON MONONUCLEAR LEUKOCYTE ISOLATED FROM HIGHER CELLS.
Authors: Dikilitas, Murat1 m.dikilitas@gmail.com, Kocyigit, Abdurrahim2, Bilinc, Hasan2, Taskin, Abdullah2
Source: Fresenius Environmental Bulletin. 2012, Vol. 21 Issue 9a, p2833-2838. 6p.
Subject Terms: *Genetic toxicology, *Pesticides, Mononuclear leukocytes, DNA damage, Blood cells
Abstract: Although it is known that the advised or lower than advised doses of eco-friendly pesticides cause a genotoxic effect on non targeted higher cells as well as on targeted organisms, their very low or ultra low doses, however, have not been tested with an efficient method. The single cell gel electrophoresis (SCGE) or comet assay was used to detect the DNA damages on higher cells in vitro exposed to insecticides such as dimethoate (100-, 50-, 10 μg ml-1), methyl parathion (90-, 45-, 5 μg ml-1), alphacypermethrin (25-, 15-, 5 μg ml-1) and dichlorvos (100-, 50-, 10 μg ml-1) in which the concentration of each insecticide was designated as "very low volume" (vlv), "very very low volume" (vvlv) and "ultra low volume" (ulv) doses, respectively. The induction of DNA damage was evident on human peripheral lymphocytes (PBL) after 1 h treatment in vitro with the above insecticides. The results showed that the "vlv" and "vvlv" doses of methyl parathion (LD50=3 μg ml-1), alphacypermethrin (LD50=57 μg ml-1) and dichlorvos (LD50=50 μg ml-1) caused significantly higher DNA damages in treated groups than those of their controls; on the other hand, dimethoate (LD50=387 μg ml-1) caused a significant damage in blood cells only in "vlv" dose. DNA damage was not detected in "ulv" doses of all insecticides. It is probable that the "vlv" or "vvlv" doses of highly toxic chemicals expressing DNA damages on blood cells might be related with their very low LD50 values, which may play an important role on the impact of environmental pollution and human health even with their very low doses. It would be also beneficial to determine the genotoxic effect of "ulv" doses of all insecticides along with the non-harmful doses of dimethoate with extended incubation period if time has potential to create extensive DNA damages. [ABSTRACT FROM AUTHOR]
Copyright of Fresenius Environmental Bulletin is the property of PRT-Parlar Research & Technology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: GENOTOXIC EFFECTS OF LOW DOSES OF PESTICIDES ON MONONUCLEAR LEUKOCYTE ISOLATED FROM HIGHER CELLS.
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  Data: <searchLink fieldCode="AR" term="%22Dikilitas%2C+Murat%22">Dikilitas, Murat</searchLink><relatesTo>1</relatesTo><i> m.dikilitas@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Kocyigit%2C+Abdurrahim%22">Kocyigit, Abdurrahim</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Bilinc%2C+Hasan%22">Bilinc, Hasan</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Taskin%2C+Abdullah%22">Taskin, Abdullah</searchLink><relatesTo>2</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Fresenius+Environmental+Bulletin%22">Fresenius Environmental Bulletin</searchLink>. 2012, Vol. 21 Issue 9a, p2833-2838. 6p.
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  Data: *<searchLink fieldCode="DE" term="%22Genetic+toxicology%22">Genetic toxicology</searchLink><br />*<searchLink fieldCode="DE" term="%22Pesticides%22">Pesticides</searchLink><br /><searchLink fieldCode="DE" term="%22Mononuclear+leukocytes%22">Mononuclear leukocytes</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+cells%22">Blood cells</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Although it is known that the advised or lower than advised doses of eco-friendly pesticides cause a genotoxic effect on non targeted higher cells as well as on targeted organisms, their very low or ultra low doses, however, have not been tested with an efficient method. The single cell gel electrophoresis (SCGE) or comet assay was used to detect the DNA damages on higher cells in vitro exposed to insecticides such as dimethoate (100-, 50-, 10 μg ml-1), methyl parathion (90-, 45-, 5 μg ml-1), alphacypermethrin (25-, 15-, 5 μg ml-1) and dichlorvos (100-, 50-, 10 μg ml-1) in which the concentration of each insecticide was designated as "very low volume" (vlv), "very very low volume" (vvlv) and "ultra low volume" (ulv) doses, respectively. The induction of DNA damage was evident on human peripheral lymphocytes (PBL) after 1 h treatment in vitro with the above insecticides. The results showed that the "vlv" and "vvlv" doses of methyl parathion (LD50=3 μg ml-1), alphacypermethrin (LD50=57 μg ml-1) and dichlorvos (LD50=50 μg ml-1) caused significantly higher DNA damages in treated groups than those of their controls; on the other hand, dimethoate (LD50=387 μg ml-1) caused a significant damage in blood cells only in "vlv" dose. DNA damage was not detected in "ulv" doses of all insecticides. It is probable that the "vlv" or "vvlv" doses of highly toxic chemicals expressing DNA damages on blood cells might be related with their very low LD50 values, which may play an important role on the impact of environmental pollution and human health even with their very low doses. It would be also beneficial to determine the genotoxic effect of "ulv" doses of all insecticides along with the non-harmful doses of dimethoate with extended incubation period if time has potential to create extensive DNA damages. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Fresenius Environmental Bulletin is the property of PRT-Parlar Research & Technology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Text: English
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        StartPage: 2833
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      – SubjectFull: Genetic toxicology
        Type: general
      – SubjectFull: Pesticides
        Type: general
      – SubjectFull: Mononuclear leukocytes
        Type: general
      – SubjectFull: DNA damage
        Type: general
      – SubjectFull: Blood cells
        Type: general
    Titles:
      – TitleFull: GENOTOXIC EFFECTS OF LOW DOSES OF PESTICIDES ON MONONUCLEAR LEUKOCYTE ISOLATED FROM HIGHER CELLS.
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            NameFull: Dikilitas, Murat
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            NameFull: Kocyigit, Abdurrahim
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            NameFull: Bilinc, Hasan
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              Text: 2012
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