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A year-long study of Cryptosporidium species and subtypes in recreational, drinking and wastewater from the central area of Spain.

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Title: A year-long study of Cryptosporidium species and subtypes in recreational, drinking and wastewater from the central area of Spain.
Authors: Galván, A.L.1,2 agalvandiaz@yahoo.com, Magnet, A.1 angela.magnetdavila@ceu.es, Izquierdo, F.1 ferizqui@ceu.es, Fernández Vadillo, C.3 carmen.fernandezvadillo@gmail.com, Peralta, R.H.4 rperalta@vm.uff.br, Angulo, S.3 sangulo@ceu.es, Fenoy, S.3 sfenrod@ceu.es, del Aguila, C.3 cagupue@ceu.es
Source: Science of the Total Environment. Jan2014, Vol. 468-469, p368-375. 8p.
Subject Terms: *Cryptosporidium, *Sewage disposal plants, *Aquatic microbiology, *Environmental sampling, Nucleotide sequence
Geographic Terms: Spain
Abstract: Abstract: A year-long longitudinal study was undertaken to evaluate the presence of Cryptosporidium spp. in drinking water treatment plants (DWTPs), wastewater treatment plants (WWTPs) and freshwater bathing beaches (FBBs) from the central area of Spain. Water samples were collected according to USEPA Method 1623, and concentrated by the IDEXX Filta-Max® system. Cryptosporidium species were detected based on PCR-restriction fragment length polymorphism and sequence analyses of the ssuRNA gene. C. hominis and/or C. parvum isolates were subtyped by DNA sequencing of the Gp60 gene. Among 150 samples, 23 (15.3%) were positive by IFAT and 40 (26.7%) by PCR. Cryptosporidium spp. was more frequent in WWTPs (26.2 and 50.8%) and FBBs (12.5 and 17.5%) by IFAT and PCR respectively. Effluent waters from DWTPs were negative for this parasite suggesting that they are suitable for public use. Tertiary treatment in the WWTPs demonstrated a high removal efficiency of Cryptosporidium in the samples evaluated. Cryptosporidium species identified included C. hominis, C. parvum, C. ubiquitum, C. andersoni and C. muris. Subtyping analysis revealed C. hominis IbA10G2 and IeA11G3T3 alleles, which is the first report of the latter in water samples. Cryptosporidium highest frequency was observed in winter and spring. Our data provide information about the occurrence and diversity of Cryptosporidium in water of human use from the central area of Spain. [Copyright &y& Elsevier]
Copyright of Science of the Total Environment is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: A year-long study of Cryptosporidium species and subtypes in recreational, drinking and wastewater from the central area of Spain.
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  Data: <searchLink fieldCode="AR" term="%22Galván%2C+A%2EL%2E%22">Galván, A.L.</searchLink><relatesTo>1,2</relatesTo><i> agalvandiaz@yahoo.com</i><br /><searchLink fieldCode="AR" term="%22Magnet%2C+A%2E%22">Magnet, A.</searchLink><relatesTo>1</relatesTo><i> angela.magnetdavila@ceu.es</i><br /><searchLink fieldCode="AR" term="%22Izquierdo%2C+F%2E%22">Izquierdo, F.</searchLink><relatesTo>1</relatesTo><i> ferizqui@ceu.es</i><br /><searchLink fieldCode="AR" term="%22Fernández+Vadillo%2C+C%2E%22">Fernández Vadillo, C.</searchLink><relatesTo>3</relatesTo><i> carmen.fernandezvadillo@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Peralta%2C+R%2EH%2E%22">Peralta, R.H.</searchLink><relatesTo>4</relatesTo><i> rperalta@vm.uff.br</i><br /><searchLink fieldCode="AR" term="%22Angulo%2C+S%2E%22">Angulo, S.</searchLink><relatesTo>3</relatesTo><i> sangulo@ceu.es</i><br /><searchLink fieldCode="AR" term="%22Fenoy%2C+S%2E%22">Fenoy, S.</searchLink><relatesTo>3</relatesTo><i> sfenrod@ceu.es</i><br /><searchLink fieldCode="AR" term="%22del+Aguila%2C+C%2E%22">del Aguila, C.</searchLink><relatesTo>3</relatesTo><i> cagupue@ceu.es</i>
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  Data: <searchLink fieldCode="JN" term="%22Science+of+the+Total+Environment%22">Science of the Total Environment</searchLink>. Jan2014, Vol. 468-469, p368-375. 8p.
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  Data: *<searchLink fieldCode="DE" term="%22Cryptosporidium%22">Cryptosporidium</searchLink><br />*<searchLink fieldCode="DE" term="%22Sewage+disposal+plants%22">Sewage disposal plants</searchLink><br />*<searchLink fieldCode="DE" term="%22Aquatic+microbiology%22">Aquatic microbiology</searchLink><br />*<searchLink fieldCode="DE" term="%22Environmental+sampling%22">Environmental sampling</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+sequence%22">Nucleotide sequence</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Spain%22">Spain</searchLink>
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  Data: Abstract: A year-long longitudinal study was undertaken to evaluate the presence of Cryptosporidium spp. in drinking water treatment plants (DWTPs), wastewater treatment plants (WWTPs) and freshwater bathing beaches (FBBs) from the central area of Spain. Water samples were collected according to USEPA Method 1623, and concentrated by the IDEXX Filta-Max® system. Cryptosporidium species were detected based on PCR-restriction fragment length polymorphism and sequence analyses of the ssuRNA gene. C. hominis and/or C. parvum isolates were subtyped by DNA sequencing of the Gp60 gene. Among 150 samples, 23 (15.3%) were positive by IFAT and 40 (26.7%) by PCR. Cryptosporidium spp. was more frequent in WWTPs (26.2 and 50.8%) and FBBs (12.5 and 17.5%) by IFAT and PCR respectively. Effluent waters from DWTPs were negative for this parasite suggesting that they are suitable for public use. Tertiary treatment in the WWTPs demonstrated a high removal efficiency of Cryptosporidium in the samples evaluated. Cryptosporidium species identified included C. hominis, C. parvum, C. ubiquitum, C. andersoni and C. muris. Subtyping analysis revealed C. hominis IbA10G2 and IeA11G3T3 alleles, which is the first report of the latter in water samples. Cryptosporidium highest frequency was observed in winter and spring. Our data provide information about the occurrence and diversity of Cryptosporidium in water of human use from the central area of Spain. [Copyright &y& Elsevier]
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  Data: <i>Copyright of Science of the Total Environment is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.scitotenv.2013.08.053
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      – Code: eng
        Text: English
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        PageCount: 8
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      – SubjectFull: Cryptosporidium
        Type: general
      – SubjectFull: Sewage disposal plants
        Type: general
      – SubjectFull: Aquatic microbiology
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      – SubjectFull: Environmental sampling
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      – SubjectFull: Nucleotide sequence
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      – SubjectFull: Spain
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              Text: Jan2014
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