Novel loading protocol combines highly efficient encapsulation of exogenous therapeutic toxin with preservation of extracellular vesicles properties, uptake and cargo activity. - Zuppone, Stefania; Zarovni, Natasa; Noguchi, Kosuke; Loria, Francesca; Morasso, Carlo; Lõhmus, Andres; Nakase, Ikuhiko; Vago, Riccardo (Discover Nano, 2024)