Direct Salmonella injection into enteroid cells allows the study of host–pathogen interactions in the cytosol with high spatiotemporal resolution. - Ernst, Chantal; Andreassen, Patrick R.; Giger, Gabriel H.; Nguyen, Bidong D.; Gäbelein, Christoph G.; Guillaume-Gentil, Orane; Fattinger, Stefan A.; Sellin, Mikael E.; Hardt, Wolf-Dietrich; Vorholt, Julia A. (PLoS Biology, 2024)