Essential Genes Discovery in Microorganisms by Transposon-Directed Sequencing (Tn-Seq): Experimental Approaches, Major Goals, and Future Perspectives. - Fernández-García, Gemma; Valdés-Chiara, Paula; Villazán-Gamonal, Patricia; Alonso-Fernández, Sergio; Manteca, Angel (International Journal of Molecular Sciences, 2024)