Developmental Expression of Calcium Buffering Proteins in Central Auditory Pathways of Normal Hearing and Congenitally Deaf Mice
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| Title: | Developmental Expression of Calcium Buffering Proteins in Central Auditory Pathways of Normal Hearing and Congenitally Deaf Mice |
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| Authors: | Deardorff, Adam S. |
| Advisors: | Fyffe, Robert E.W. |
| Summary: | These experiments analyze differences in synaptic development in central auditory pathways between normal hearing (CBA/J) and congenitally deaf (dn/dn) mice, which provide valuable insight into central synaptic plasticity corresponding to human congenital deafness. Immunofluorescent analysis of the developmental expression of the calcium buffering proteins calretinin, calbindin d-28k, and parvalbumin at various postnatal time points was performed to assess the effects of altered neural activity on the level and/or pattern of protein expression within these nuclei. Results indicate that the pattern of calbindin and parvalbumin is unaffected by congenital deafness in dn/dn mice. However, the pattern of calretinin expression in the MNTB during development of dn/dn mice differed significantly from that of CBA/J mice, indicating that calcium buffering may be impaired in these synapses without appropriate afferent stimulation. |
| URL: | http://rave.ohiolink.edu/etdc/view?acc_num=wright1276870379 |
| Database: | OpenDissertations |
| Abstract: | These experiments analyze differences in synaptic development in central auditory pathways between normal hearing (CBA/J) and congenitally deaf (dn/dn) mice, which provide valuable insight into central synaptic plasticity corresponding to human congenital deafness. Immunofluorescent analysis of the developmental expression of the calcium buffering proteins calretinin, calbindin d-28k, and parvalbumin at various postnatal time points was performed to assess the effects of altered neural activity on the level and/or pattern of protein expression within these nuclei. Results indicate that the pattern of calbindin and parvalbumin is unaffected by congenital deafness in dn/dn mice. However, the pattern of calretinin expression in the MNTB during development of dn/dn mice differed significantly from that of CBA/J mice, indicating that calcium buffering may be impaired in these synapses without appropriate afferent stimulation. |
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