Expression Profiling of Estrogenic Compounds Using a Sheepshead Minnow cDNA Macroarray.
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| Title: | Expression Profiling of Estrogenic Compounds Using a Sheepshead Minnow cDNA Macroarray. |
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| Authors: | Larkin, Patrick, Folmar, Leroy C., Hemmer, Michael J., Poston, Arianna J., Denslow, Nancy D. |
| Source: | Environmental Health Perspectives. May2003 Toxicogenomics, Vol. 111 Issue 6, p839. 8p. |
| Subjects: | Estrogen, DNA fingerprinting, Sheepshead minnow, DNA microarrays |
| Abstract: | A variety of anthropogenic compounds are capable of binding to the estrogen receptor (ER) of vertebrate species. Binding of these chemicals to the ER can interfere with homeostasis by altering normal gene expression patterns. The purpose of this study was to characterize the expression of 30 genes using a sheepshead minnow (Cyprinodon variegatus) cDNA macroarray. Many of the genes on the array were previously identified by differential display reverse transcriptase-polymerase chain reaction to be upregulated or downregulated in sheepshead minnows treated through aqueous exposure to known or suspected estrogenic chemicals. The results of this study show that 17β-estradiol (E[sub 2]), 17α-ethinyl estradiol (EE[sub 2]), diethylstilbestrol (DES), and methoxychlor (MXC) have similar genetic signatures for the 30 genes examined. The genetic signature of fish treated with p-nonylphenol was identical in pattern to that in fish treated with E[sub 2], EE[sub 2], DES, and MXC except for the additional upregulation of a cDNA clone that shares similarity to ubiquitin-conjugating enzyme 9. Endosulfan produced results that resembled the gene expression patterns of untreated control fish with exception of the upregulation of estrogen receptor ct and the downregulation of a cDNA clone that shares similarity to 3-hydroxy-3-methylglutaryl-coenzyme A reductase. We show that our estrogen-responsive cDNA macroarray can detect dose-dependent changes in gene expression patterns in fish treated with EE[sub 2]. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Health Perspectives is the property of National Institute of Environmental Health Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 10036465 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Expression Profiling of Estrogenic Compounds Using a Sheepshead Minnow cDNA Macroarray. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Larkin%2C+Patrick%22">Larkin, Patrick</searchLink><br /><searchLink fieldCode="AR" term="%22Folmar%2C+Leroy+C%2E%22">Folmar, Leroy C.</searchLink><br /><searchLink fieldCode="AR" term="%22Hemmer%2C+Michael+J%2E%22">Hemmer, Michael J.</searchLink><br /><searchLink fieldCode="AR" term="%22Poston%2C+Arianna+J%2E%22">Poston, Arianna J.</searchLink><br /><searchLink fieldCode="AR" term="%22Denslow%2C+Nancy+D%2E%22">Denslow, Nancy D.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Health+Perspectives%22">Environmental Health Perspectives</searchLink>. May2003 Toxicogenomics, Vol. 111 Issue 6, p839. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Estrogen%22">Estrogen</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+fingerprinting%22">DNA fingerprinting</searchLink><br /><searchLink fieldCode="DE" term="%22Sheepshead+minnow%22">Sheepshead minnow</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+microarrays%22">DNA microarrays</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: A variety of anthropogenic compounds are capable of binding to the estrogen receptor (ER) of vertebrate species. Binding of these chemicals to the ER can interfere with homeostasis by altering normal gene expression patterns. The purpose of this study was to characterize the expression of 30 genes using a sheepshead minnow (Cyprinodon variegatus) cDNA macroarray. Many of the genes on the array were previously identified by differential display reverse transcriptase-polymerase chain reaction to be upregulated or downregulated in sheepshead minnows treated through aqueous exposure to known or suspected estrogenic chemicals. The results of this study show that 17β-estradiol (E[sub 2]), 17α-ethinyl estradiol (EE[sub 2]), diethylstilbestrol (DES), and methoxychlor (MXC) have similar genetic signatures for the 30 genes examined. The genetic signature of fish treated with p-nonylphenol was identical in pattern to that in fish treated with E[sub 2], EE[sub 2], DES, and MXC except for the additional upregulation of a cDNA clone that shares similarity to ubiquitin-conjugating enzyme 9. Endosulfan produced results that resembled the gene expression patterns of untreated control fish with exception of the upregulation of estrogen receptor ct and the downregulation of a cDNA clone that shares similarity to 3-hydroxy-3-methylglutaryl-coenzyme A reductase. We show that our estrogen-responsive cDNA macroarray can detect dose-dependent changes in gene expression patterns in fish treated with EE[sub 2]. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Health Perspectives is the property of National Institute of Environmental Health Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1289/ehp.111-1241505 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 839 Subjects: – SubjectFull: Estrogen Type: general – SubjectFull: DNA fingerprinting Type: general – SubjectFull: Sheepshead minnow Type: general – SubjectFull: DNA microarrays Type: general Titles: – TitleFull: Expression Profiling of Estrogenic Compounds Using a Sheepshead Minnow cDNA Macroarray. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Larkin, Patrick – PersonEntity: Name: NameFull: Folmar, Leroy C. – PersonEntity: Name: NameFull: Hemmer, Michael J. – PersonEntity: Name: NameFull: Poston, Arianna J. – PersonEntity: Name: NameFull: Denslow, Nancy D. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 05 Text: May2003 Toxicogenomics Type: published Y: 2003 Identifiers: – Type: issn-print Value: 00916765 Numbering: – Type: volume Value: 111 – Type: issue Value: 6 Titles: – TitleFull: Environmental Health Perspectives Type: main |
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