Taylor dispersion and the position-to-time conversion in microfluidic mixing devices.
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| Title: | Taylor dispersion and the position-to-time conversion in microfluidic mixing devices. |
|---|---|
| Authors: | Wunderlich, B.1, Nettels, D.1, Schuler, B.1 |
| Source: | Lab on a Chip. 2014, Vol. 14 Issue 1, p219-228. 10p. |
| Subjects: | Biochips, Micrometry, Microfluidics, Microfluidic analytical techniques, Miniature electronic equipment, Microbiology, Chemical biology |
| Abstract: | Microfluidic mixing devices are increasingly popular tools for probing the non-equilibrium dynamics of biomolecular systems. Commonly, hydrodynamic focusing is used to reduce the length scales that limit the time of diffusive mixing in the laminar flow regime, such that even sub-millisecond dead times for triggering a reaction have been achieved. Detection of a suitable signal at different points along the channel downstream of the mixing region, corresponding to different times after mixing, then allows the kinetics of the reaction to be obtained. However, the requisite accurate conversion of the positions in the channel to times after mixing is complicated by Taylor dispersion, the combined effect of diffusion and shear flow on the dispersion of the molecules in the microfluidic device. As a result, an accurate position-to-time conversion has only been possible in the limiting regimes, i.e. for very early times, where sample diffusion can be neglected, and for very long times, where the molecules have uniformly sampled the entire channel cross-section. Here, we use detailed three-dimensional, time-dependent finite-element calculations to obtain an accurate position-to-time conversion that bridges these two limits and allows us to quantify the effects of Taylor dispersion on the time resolution of a representative mixing device optimized for single-molecule fluorescence detection. The accuracy of the calculations is confirmed by direct comparison of the calculated velocity field with dual-focus fluorescence correlation spectroscopy measurements. [ABSTRACT FROM AUTHOR] |
| Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 100403838 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Taylor dispersion and the position-to-time conversion in microfluidic mixing devices. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wunderlich%2C+B%2E%22">Wunderlich, B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nettels%2C+D%2E%22">Nettels, D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Schuler%2C+B%2E%22">Schuler, B.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lab+on+a+Chip%22">Lab on a Chip</searchLink>. 2014, Vol. 14 Issue 1, p219-228. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Biochips%22">Biochips</searchLink><br /><searchLink fieldCode="DE" term="%22Micrometry%22">Micrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Microfluidics%22">Microfluidics</searchLink><br /><searchLink fieldCode="DE" term="%22Microfluidic+analytical+techniques%22">Microfluidic analytical techniques</searchLink><br /><searchLink fieldCode="DE" term="%22Miniature+electronic+equipment%22">Miniature electronic equipment</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+biology%22">Chemical biology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Microfluidic mixing devices are increasingly popular tools for probing the non-equilibrium dynamics of biomolecular systems. Commonly, hydrodynamic focusing is used to reduce the length scales that limit the time of diffusive mixing in the laminar flow regime, such that even sub-millisecond dead times for triggering a reaction have been achieved. Detection of a suitable signal at different points along the channel downstream of the mixing region, corresponding to different times after mixing, then allows the kinetics of the reaction to be obtained. However, the requisite accurate conversion of the positions in the channel to times after mixing is complicated by Taylor dispersion, the combined effect of diffusion and shear flow on the dispersion of the molecules in the microfluidic device. As a result, an accurate position-to-time conversion has only been possible in the limiting regimes, i.e. for very early times, where sample diffusion can be neglected, and for very long times, where the molecules have uniformly sampled the entire channel cross-section. Here, we use detailed three-dimensional, time-dependent finite-element calculations to obtain an accurate position-to-time conversion that bridges these two limits and allows us to quantify the effects of Taylor dispersion on the time resolution of a representative mixing device optimized for single-molecule fluorescence detection. The accuracy of the calculations is confirmed by direct comparison of the calculated velocity field with dual-focus fluorescence correlation spectroscopy measurements. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1039/c3lc51002f Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 219 Subjects: – SubjectFull: Biochips Type: general – SubjectFull: Micrometry Type: general – SubjectFull: Microfluidics Type: general – SubjectFull: Microfluidic analytical techniques Type: general – SubjectFull: Miniature electronic equipment Type: general – SubjectFull: Microbiology Type: general – SubjectFull: Chemical biology Type: general Titles: – TitleFull: Taylor dispersion and the position-to-time conversion in microfluidic mixing devices. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wunderlich, B. – PersonEntity: Name: NameFull: Nettels, D. – PersonEntity: Name: NameFull: Schuler, B. IsPartOfRelationships: – BibEntity: Dates: – D: 07 M: 01 Text: 2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 14730197 Numbering: – Type: volume Value: 14 – Type: issue Value: 1 Titles: – TitleFull: Lab on a Chip Type: main |
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