View in EDS

Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments.

Saved in:
Bibliographic Details
Title: Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments.
Authors: Wong, Shirley Y.1, Paschos, Athanasios1, Gupta, Radhey S.2, Schellhom, Herb E.1 schell@mcmaster.ca
Source: Environmental Science & Technology. 10/7/2014, Vol. 48 Issue 19, p11462-11470. 9p.
Subjects: Fresh water, Escherichia coli O157:H7, Gastrointestinal diseases, Hemolytic-uremic syndrome, Public health, Biomarkers, Pathogenic microorganisms, Diagnostic microbiology
Abstract: Enterohemorrhagic Escherichia coli O157:H7 is responsible for many outbreaks of gastrointestmal illness and hemolytic uremic syndrome worldwide. Monitoring this pathogen in food and water supplies is an unportant public health issue. Highly conserved genetic markers, which are characteristic for specific strains can provide direct identification of target pathogens. In this study, we examined a new detection strategy for pathogenic strains of E. coli 0157:H7 serotype based on a conserved signature insertion/deletion (CSI located in the yhiX gene using TaqMan-probe-based quantitative PCR (qPCR). The qPCR assay was linear from 1.0 X 102 to 1.0 X 107 genome copies and was specific to Ol57:H7 when tested against a panel of 15 non-0157:H7 E col, The assay also maintained detection sensitivity in the presence of competing E coli K-12, heterologous nontarget DNA spiked in at a 1000-fold and 800-fold excess of target DNA, respectively, demonstrating the assay-s ability to detect E coli 0157:H7 in the presence of high levels of background DNA. This study thus validates the use of strain-specific CSIs as a new class of diagnostic marker for pathogen detection. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Science & Technology is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:Enterohemorrhagic Escherichia coli O157:H7 is responsible for many outbreaks of gastrointestmal illness and hemolytic uremic syndrome worldwide. Monitoring this pathogen in food and water supplies is an unportant public health issue. Highly conserved genetic markers, which are characteristic for specific strains can provide direct identification of target pathogens. In this study, we examined a new detection strategy for pathogenic strains of E. coli 0157:H7 serotype based on a conserved signature insertion/deletion (CSI located in the yhiX gene using TaqMan-probe-based quantitative PCR (qPCR). The qPCR assay was linear from 1.0 X 102 to 1.0 X 107 genome copies and was specific to Ol57:H7 when tested against a panel of 15 non-0157:H7 E col, The assay also maintained detection sensitivity in the presence of competing E coli K-12, heterologous nontarget DNA spiked in at a 1000-fold and 800-fold excess of target DNA, respectively, demonstrating the assay-s ability to detect E coli 0157:H7 in the presence of high levels of background DNA. This study thus validates the use of strain-specific CSIs as a new class of diagnostic marker for pathogen detection. [ABSTRACT FROM AUTHOR]
ISSN:0013936X
DOI:10.1021/es502794h