Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments.
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| Title: | Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments. |
|---|---|
| Authors: | Wong, Shirley Y.1, Paschos, Athanasios1, Gupta, Radhey S.2, Schellhom, Herb E.1 schell@mcmaster.ca |
| Source: | Environmental Science & Technology. 10/7/2014, Vol. 48 Issue 19, p11462-11470. 9p. |
| Subjects: | Fresh water, Escherichia coli O157:H7, Gastrointestinal diseases, Hemolytic-uremic syndrome, Public health, Biomarkers, Pathogenic microorganisms, Diagnostic microbiology |
| Abstract: | Enterohemorrhagic Escherichia coli O157:H7 is responsible for many outbreaks of gastrointestmal illness and hemolytic uremic syndrome worldwide. Monitoring this pathogen in food and water supplies is an unportant public health issue. Highly conserved genetic markers, which are characteristic for specific strains can provide direct identification of target pathogens. In this study, we examined a new detection strategy for pathogenic strains of E. coli 0157:H7 serotype based on a conserved signature insertion/deletion (CSI located in the yhiX gene using TaqMan-probe-based quantitative PCR (qPCR). The qPCR assay was linear from 1.0 X 102 to 1.0 X 107 genome copies and was specific to Ol57:H7 when tested against a panel of 15 non-0157:H7 E col, The assay also maintained detection sensitivity in the presence of competing E coli K-12, heterologous nontarget DNA spiked in at a 1000-fold and 800-fold excess of target DNA, respectively, demonstrating the assay-s ability to detect E coli 0157:H7 in the presence of high levels of background DNA. This study thus validates the use of strain-specific CSIs as a new class of diagnostic marker for pathogen detection. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Science & Technology is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 100684245 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wong%2C+Shirley+Y%2E%22">Wong, Shirley Y.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Paschos%2C+Athanasios%22">Paschos, Athanasios</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gupta%2C+Radhey+S%2E%22">Gupta, Radhey S.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Schellhom%2C+Herb+E%2E%22">Schellhom, Herb E.</searchLink><relatesTo>1</relatesTo><i> schell@mcmaster.ca</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Science+%26+Technology%22">Environmental Science & Technology</searchLink>. 10/7/2014, Vol. 48 Issue 19, p11462-11470. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Fresh+water%22">Fresh water</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli+O157%3AH7%22">Escherichia coli O157:H7</searchLink><br /><searchLink fieldCode="DE" term="%22Gastrointestinal+diseases%22">Gastrointestinal diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Hemolytic-uremic+syndrome%22">Hemolytic-uremic syndrome</searchLink><br /><searchLink fieldCode="DE" term="%22Public+health%22">Public health</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Pathogenic+microorganisms%22">Pathogenic microorganisms</searchLink><br /><searchLink fieldCode="DE" term="%22Diagnostic+microbiology%22">Diagnostic microbiology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Enterohemorrhagic Escherichia coli O157:H7 is responsible for many outbreaks of gastrointestmal illness and hemolytic uremic syndrome worldwide. Monitoring this pathogen in food and water supplies is an unportant public health issue. Highly conserved genetic markers, which are characteristic for specific strains can provide direct identification of target pathogens. In this study, we examined a new detection strategy for pathogenic strains of E. coli 0157:H7 serotype based on a conserved signature insertion/deletion (CSI located in the yhiX gene using TaqMan-probe-based quantitative PCR (qPCR). The qPCR assay was linear from 1.0 X 102 to 1.0 X 107 genome copies and was specific to Ol57:H7 when tested against a panel of 15 non-0157:H7 E col, The assay also maintained detection sensitivity in the presence of competing E coli K-12, heterologous nontarget DNA spiked in at a 1000-fold and 800-fold excess of target DNA, respectively, demonstrating the assay-s ability to detect E coli 0157:H7 in the presence of high levels of background DNA. This study thus validates the use of strain-specific CSIs as a new class of diagnostic marker for pathogen detection. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Science & Technology is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/es502794h Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 11462 Subjects: – SubjectFull: Fresh water Type: general – SubjectFull: Escherichia coli O157:H7 Type: general – SubjectFull: Gastrointestinal diseases Type: general – SubjectFull: Hemolytic-uremic syndrome Type: general – SubjectFull: Public health Type: general – SubjectFull: Biomarkers Type: general – SubjectFull: Pathogenic microorganisms Type: general – SubjectFull: Diagnostic microbiology Type: general Titles: – TitleFull: Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wong, Shirley Y. – PersonEntity: Name: NameFull: Paschos, Athanasios – PersonEntity: Name: NameFull: Gupta, Radhey S. – PersonEntity: Name: NameFull: Schellhom, Herb E. IsPartOfRelationships: – BibEntity: Dates: – D: 07 M: 10 Text: 10/7/2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 0013936X Numbering: – Type: volume Value: 48 – Type: issue Value: 19 Titles: – TitleFull: Environmental Science & Technology Type: main |
| ResultId | 1 |
