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Effect of low-level laser therapy on oral keratinocytes exposed to bisphosphonate.

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Title: Effect of low-level laser therapy on oral keratinocytes exposed to bisphosphonate.
Authors: Lee, Jae-Yeol id9753153@gmail.com, Kim, In-Ryoung1 biowool77@gmail.com, Park, Bong-Soo1 parkbs@pusan.ac.kr, Kim, Yong-Deok2 ydkimdds@pusan.ac.kr, Chung, In-Kyo2 inkchung@pusan.ac.kr, Song, Jae-Min2 gabriel001@hanmail.net, Shin, Sang-Hun2 ssh8080@pusan.ac.kr
Source: Lasers in Medical Science. Feb2015, Vol. 30 Issue 2, p635-643. 9p.
Subjects: Laser endoscopy, Keratinocytes, Diphosphonates, Irradiation, Immunofluorescence
Abstract: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a side effect of bisphosphonate therapy. However, its pathophysiology is not yet fully elucidated, and effective treatment of BRONJ remains unclear. The aim of this study is to investigate the effects of alendronate on oral keratinocytes and of low-level laser therapy (LLLT) on alendronate-treated keratinocytes, specifically by evaluating their viability, apoptosis, and wound healing function after irradiation. Oral keratinocyte cells (HaCaT) were exposed to 25 μM alendronate. Then, laser irradiation was performed with a low-level Ga-Al-As laser ( λ = 808 ± 3 nm, 80 mW, and 80 mA; NDLux, Seoul, Korea) using 1.2 J/cm energy dose. Viability was analyzed using MTT assay. Apoptosis was measured by Hoechst staining, caspase assay. Changes in secretion of IL-8, VEGF, and collagen type I were studied by ELISA and immunofluorescence microscopy. Scratch wound assays were also performed to measure cellular migration. Our results show that alendronate inhibits keratinocyte viability, expression of IL-8, VEGF, and collagen type I which are intimately related to healing events and cell migration while promoting apoptosis. Our results serve to demonstrate the utility of LLLT in partially overcoming the inhibitory effects of this bisphosphonate. From these results, the authors believe that the present study will provide an experimental basis for a fuller explanation of the clinical effects of LLLT as a BRONJ treatment modality. [ABSTRACT FROM AUTHOR]
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Effect of low-level laser therapy on oral keratinocytes exposed to bisphosphonate.
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  Data: <searchLink fieldCode="AR" term="%22Lee%2C+Jae-Yeol%22">Lee, Jae-Yeol</searchLink><i> id9753153@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Kim%2C+In-Ryoung%22">Kim, In-Ryoung</searchLink><relatesTo>1</relatesTo><i> biowool77@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Park%2C+Bong-Soo%22">Park, Bong-Soo</searchLink><relatesTo>1</relatesTo><i> parkbs@pusan.ac.kr</i><br /><searchLink fieldCode="AR" term="%22Kim%2C+Yong-Deok%22">Kim, Yong-Deok</searchLink><relatesTo>2</relatesTo><i> ydkimdds@pusan.ac.kr</i><br /><searchLink fieldCode="AR" term="%22Chung%2C+In-Kyo%22">Chung, In-Kyo</searchLink><relatesTo>2</relatesTo><i> inkchung@pusan.ac.kr</i><br /><searchLink fieldCode="AR" term="%22Song%2C+Jae-Min%22">Song, Jae-Min</searchLink><relatesTo>2</relatesTo><i> gabriel001@hanmail.net</i><br /><searchLink fieldCode="AR" term="%22Shin%2C+Sang-Hun%22">Shin, Sang-Hun</searchLink><relatesTo>2</relatesTo><i> ssh8080@pusan.ac.kr</i>
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  Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. Feb2015, Vol. 30 Issue 2, p635-643. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Laser+endoscopy%22">Laser endoscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Keratinocytes%22">Keratinocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Diphosphonates%22">Diphosphonates</searchLink><br /><searchLink fieldCode="DE" term="%22Irradiation%22">Irradiation</searchLink><br /><searchLink fieldCode="DE" term="%22Immunofluorescence%22">Immunofluorescence</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a side effect of bisphosphonate therapy. However, its pathophysiology is not yet fully elucidated, and effective treatment of BRONJ remains unclear. The aim of this study is to investigate the effects of alendronate on oral keratinocytes and of low-level laser therapy (LLLT) on alendronate-treated keratinocytes, specifically by evaluating their viability, apoptosis, and wound healing function after irradiation. Oral keratinocyte cells (HaCaT) were exposed to 25 μM alendronate. Then, laser irradiation was performed with a low-level Ga-Al-As laser ( λ = 808 ± 3 nm, 80 mW, and 80 mA; NDLux, Seoul, Korea) using 1.2 J/cm energy dose. Viability was analyzed using MTT assay. Apoptosis was measured by Hoechst staining, caspase assay. Changes in secretion of IL-8, VEGF, and collagen type I were studied by ELISA and immunofluorescence microscopy. Scratch wound assays were also performed to measure cellular migration. Our results show that alendronate inhibits keratinocyte viability, expression of IL-8, VEGF, and collagen type I which are intimately related to healing events and cell migration while promoting apoptosis. Our results serve to demonstrate the utility of LLLT in partially overcoming the inhibitory effects of this bisphosphonate. From these results, the authors believe that the present study will provide an experimental basis for a fuller explanation of the clinical effects of LLLT as a BRONJ treatment modality. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Type: general
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              Text: Feb2015
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