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Preparation and identification of transfer factor specific to Staphylococcus aureus in vitro.

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Title: Preparation and identification of transfer factor specific to Staphylococcus aureus in vitro.
Authors: Zhou, Jianwei1, Kong, Cui2, Wang, Xiukui3, Shao, Jun2, Feng, Lin4, Zhang, Zhaocai1
Source: Biotechnology & Applied Biochemistry. Jan2015, Vol. 62 Issue 1, p112-120. 9p.
Subjects: Transfer factor (Immunology), Staphylococcus aureus infections, Phagocytosis, Immunity, Immunological adjuvants, Therapeutics
Abstract: The objective of the study was to explore the methods for preparing transfer factor specific to Staphylococcus aureus ( SA-STF) in vitro. Under the optimum conditions, the spleen cells of rabbits were immunized with SA in vitro to prepare SA-STF, and the immune activities were identified with the phagocytosis and sterilization, skin delayed-type hypersensitivity, and immune protection tests. The concentration of polypeptide was 2.26 ± 0.27 mg/mL, and ribose was 0.684 ± 0.094 mg/mL. The phagocytosis and sterilization rates of the STF group were 70.9 ± 12.4% and 62.1 ± 12.2%, respectively, and compared with the non-specific transfer factor ( NTF) group, there were no significant differences ( P = 0.074 and 0.069, respectively). The skin was inflamed and marked nodules formed at the injection site in the mice of the STF group rather than the NTF and control groups. The survival rate of the STF-1 group was significantly higher than the survival rates of the STF-2 ( P = 0.024) and NTF groups ( P = 0.016). SA-STF was prepared and characterized successfully in vitro, and it probably is a biological candidate for therapy or adjuvant therapy for diseases caused by Staphylococcus aureus. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Preparation and identification of transfer factor specific to Staphylococcus aureus in vitro.
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  Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Jan2015, Vol. 62 Issue 1, p112-120. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Transfer+factor+%28Immunology%29%22">Transfer factor (Immunology)</searchLink><br /><searchLink fieldCode="DE" term="%22Staphylococcus+aureus+infections%22">Staphylococcus aureus infections</searchLink><br /><searchLink fieldCode="DE" term="%22Phagocytosis%22">Phagocytosis</searchLink><br /><searchLink fieldCode="DE" term="%22Immunity%22">Immunity</searchLink><br /><searchLink fieldCode="DE" term="%22Immunological+adjuvants%22">Immunological adjuvants</searchLink><br /><searchLink fieldCode="DE" term="%22Therapeutics%22">Therapeutics</searchLink>
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  Label: Abstract
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  Data: The objective of the study was to explore the methods for preparing transfer factor specific to Staphylococcus aureus ( SA-STF) in vitro. Under the optimum conditions, the spleen cells of rabbits were immunized with SA in vitro to prepare SA-STF, and the immune activities were identified with the phagocytosis and sterilization, skin delayed-type hypersensitivity, and immune protection tests. The concentration of polypeptide was 2.26 ± 0.27 mg/mL, and ribose was 0.684 ± 0.094 mg/mL. The phagocytosis and sterilization rates of the STF group were 70.9 ± 12.4% and 62.1 ± 12.2%, respectively, and compared with the non-specific transfer factor ( NTF) group, there were no significant differences ( P = 0.074 and 0.069, respectively). The skin was inflamed and marked nodules formed at the injection site in the mice of the STF group rather than the NTF and control groups. The survival rate of the STF-1 group was significantly higher than the survival rates of the STF-2 ( P = 0.024) and NTF groups ( P = 0.016). SA-STF was prepared and characterized successfully in vitro, and it probably is a biological candidate for therapy or adjuvant therapy for diseases caused by Staphylococcus aureus. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1002/bab.1241
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        Text: English
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        Type: general
      – SubjectFull: Staphylococcus aureus infections
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      – SubjectFull: Phagocytosis
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            NameFull: Kong, Cui
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              Text: Jan2015
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