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Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved.

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Title: Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved.
Authors: Bedford, Andrea1, Chen, Tao1,2, Huynh, Evanna1, Zhu, Cuilan1, Medeiros, Samantha1, Wey, Doug1, de Lange, Cornelis1, Li, Julang1 jli@uoguelph.ca
Source: Journal of Biotechnology. Feb2015, Vol. 196, p9-19. 11p.
Subjects: Epidermal growth factor, Intestinal development, Laboratory swine, Lactococcus lactis, Fermentation
Abstract: We have previously generated epidermal factor expressing Lactococcus lactis (EGF-LL) using a bioengineering approach, and shown that EGF-LL fermentation supernatant enhanced newly weaned pigs growth. The objective of the current study was to further understand the mechanisms behind this improved performance. Sixty-four piglets were weaned at 3 weeks of age and then fed ad libitum according to a 2-phase feeding program. Four pens with 8 pigs per pen were assigned to each of two treatments for 3 weeks: (1) EGF containing supernatant from EGF-LL culture (SuperEGF) or (2) blank M17GE media (Control). Consistent with previous findings, SuperEGF pigs had an increased average daily gain during week 3 post-weaning (433.4 ± 10.86 vs 388.7 ± 7.76 g; P < 0.05) and overall gain:feed ratio (0.757 ± 0.03 vs 0.677 ± 0.01 kg/kg, P < 0.05). Moreover, jejunal structure development was enhanced, and inflammation index was minimized in SuperEGF pigs as indicated by increased villi height ( P < 0.05), decreased lamina propria width ( P < 0.05), and higher expression of anti-inflammatory cytokine, IL-13 ( P < 0.05). Further, goblet cell numbers and Muc2 levels were increased in SuperEGF pigs. Interestingly, the weaning-induced decrease of glucose cotransporter sodium-glucose linked transporter 1 (SGLT1) and glucagon-like peptide-2 (GLP2) levels was reversed by SuperEGF supplementation. Our findings add to our understanding of the mechanisms behind enhancing piglet performance by EGF containing fermentation product. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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PubType: Academic Journal
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  Data: Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Bedford%2C+Andrea%22&quot;&gt;Bedford, Andrea&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Chen%2C+Tao%22&quot;&gt;Chen, Tao&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Huynh%2C+Evanna%22&quot;&gt;Huynh, Evanna&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhu%2C+Cuilan%22&quot;&gt;Zhu, Cuilan&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Medeiros%2C+Samantha%22&quot;&gt;Medeiros, Samantha&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wey%2C+Doug%22&quot;&gt;Wey, Doug&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22de+Lange%2C+Cornelis%22&quot;&gt;de Lange, Cornelis&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Li%2C+Julang%22&quot;&gt;Li, Julang&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;i&gt; jli@uoguelph.ca&lt;/i&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Journal+of+Biotechnology%22&quot;&gt;Journal of Biotechnology&lt;/searchLink&gt;. Feb2015, Vol. 196, p9-19. 11p.
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Epidermal+growth+factor%22&quot;&gt;Epidermal growth factor&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Intestinal+development%22&quot;&gt;Intestinal development&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Laboratory+swine%22&quot;&gt;Laboratory swine&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Lactococcus+lactis%22&quot;&gt;Lactococcus lactis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Fermentation%22&quot;&gt;Fermentation&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
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  Data: We have previously generated epidermal factor expressing Lactococcus lactis (EGF-LL) using a bioengineering approach, and shown that EGF-LL fermentation supernatant enhanced newly weaned pigs growth. The objective of the current study was to further understand the mechanisms behind this improved performance. Sixty-four piglets were weaned at 3 weeks of age and then fed ad libitum according to a 2-phase feeding program. Four pens with 8 pigs per pen were assigned to each of two treatments for 3 weeks: (1) EGF containing supernatant from EGF-LL culture (SuperEGF) or (2) blank M17GE media (Control). Consistent with previous findings, SuperEGF pigs had an increased average daily gain during week 3 post-weaning (433.4 &#177; 10.86 vs 388.7 &#177; 7.76 g; P &lt; 0.05) and overall gain:feed ratio (0.757 &#177; 0.03 vs 0.677 &#177; 0.01 kg/kg, P &lt; 0.05). Moreover, jejunal structure development was enhanced, and inflammation index was minimized in SuperEGF pigs as indicated by increased villi height ( P &lt; 0.05), decreased lamina propria width ( P &lt; 0.05), and higher expression of anti-inflammatory cytokine, IL-13 ( P &lt; 0.05). Further, goblet cell numbers and Muc2 levels were increased in SuperEGF pigs. Interestingly, the weaning-induced decrease of glucose cotransporter sodium-glucose linked transporter 1 (SGLT1) and glucagon-like peptide-2 (GLP2) levels was reversed by SuperEGF supplementation. Our findings add to our understanding of the mechanisms behind enhancing piglet performance by EGF containing fermentation product. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: &lt;i&gt;Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jbiotec.2015.01.007
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 9
    Subjects:
      – SubjectFull: Epidermal growth factor
        Type: general
      – SubjectFull: Intestinal development
        Type: general
      – SubjectFull: Laboratory swine
        Type: general
      – SubjectFull: Lactococcus lactis
        Type: general
      – SubjectFull: Fermentation
        Type: general
    Titles:
      – TitleFull: Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved.
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            NameFull: Bedford, Andrea
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            NameFull: Chen, Tao
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            NameFull: Huynh, Evanna
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            NameFull: Medeiros, Samantha
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            NameFull: Wey, Doug
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            NameFull: de Lange, Cornelis
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              Text: Feb2015
              Type: published
              Y: 2015
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              Value: 196
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            – TitleFull: Journal of Biotechnology
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