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Oxygen delivery from hyperbarically loaded microtanks extends cell viability in anoxic environments.

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Title: Oxygen delivery from hyperbarically loaded microtanks extends cell viability in anoxic environments.
Authors: Cook, Colin A.1,2, Hahn, Kathryn C.1,2, Morrissette-McAlmon, Justin B.F.1,2, Grayson, Warren L.1,2,3 wgrayson@jhmi.edu
Source: Biomaterials. Jun2015, Vol. 52, p376-384. 9p.
Subjects: Photosynthetic oxygen evolution, Umbilical veins, Stem cell culture, Regeneration (Biology)
Abstract: Oxygen diffusion limitations within nascent tissue engineered (TE) grafts lead to the development of hypoxic regions, cell death, and graft failure. Previous efforts have been made to deliver oxygen within TE scaffolds, including peroxide-doping, perfluorocarbons, and hyperbaric oxygen therapy, to mitigate these effects and help maintain post transplantation cell viability, but these have suffered from significant drawbacks. Here we present a novel approach utilizing polymeric hollow-core microspheres that can be hyperbarically loaded with oxygen and subsequently provide prolonged oxygen delivery. These oxygen carriers are termed, microtanks . With an interest in orthopedic applications, we combined microtanks within polycaprolactone to form solid phase constructs with oxygen delivery capabilities. The mathematical laws governing oxygen delivery from microtank-loaded constructs are developed along with empirical validation. Constructs achieved periods of oxygen delivery out to 6 days, which was shown to prolong the survival of human adipose derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs) as well as to enhance their cellular morphology under anoxic conditions. The results of this study suggest the microtank approach may be a feasible means of maintaining cell viability in TE scaffolds during the critical period of vascularization in vivo . [ABSTRACT FROM AUTHOR]
Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: <searchLink fieldCode="DE" term="%22Photosynthetic+oxygen+evolution%22">Photosynthetic oxygen evolution</searchLink><br /><searchLink fieldCode="DE" term="%22Umbilical+veins%22">Umbilical veins</searchLink><br /><searchLink fieldCode="DE" term="%22Stem+cell+culture%22">Stem cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22Regeneration+%28Biology%29%22">Regeneration (Biology)</searchLink>
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  Data: Oxygen diffusion limitations within nascent tissue engineered (TE) grafts lead to the development of hypoxic regions, cell death, and graft failure. Previous efforts have been made to deliver oxygen within TE scaffolds, including peroxide-doping, perfluorocarbons, and hyperbaric oxygen therapy, to mitigate these effects and help maintain post transplantation cell viability, but these have suffered from significant drawbacks. Here we present a novel approach utilizing polymeric hollow-core microspheres that can be hyperbarically loaded with oxygen and subsequently provide prolonged oxygen delivery. These oxygen carriers are termed, microtanks . With an interest in orthopedic applications, we combined microtanks within polycaprolactone to form solid phase constructs with oxygen delivery capabilities. The mathematical laws governing oxygen delivery from microtank-loaded constructs are developed along with empirical validation. Constructs achieved periods of oxygen delivery out to 6 days, which was shown to prolong the survival of human adipose derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs) as well as to enhance their cellular morphology under anoxic conditions. The results of this study suggest the microtank approach may be a feasible means of maintaining cell viability in TE scaffolds during the critical period of vascularization in vivo . [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.biomaterials.2015.02.036
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      – Code: eng
        Text: English
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        PageCount: 9
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      – SubjectFull: Photosynthetic oxygen evolution
        Type: general
      – SubjectFull: Umbilical veins
        Type: general
      – SubjectFull: Stem cell culture
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      – SubjectFull: Regeneration (Biology)
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      – TitleFull: Oxygen delivery from hyperbarically loaded microtanks extends cell viability in anoxic environments.
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            NameFull: Morrissette-McAlmon, Justin B.F.
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            – D: 01
              M: 06
              Text: Jun2015
              Type: published
              Y: 2015
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