13C magnetic resonance spectroscopic imaging of hyperpolarized [1-13C, U-2H5] ethanol oxidation can be used to assess aldehyde dehydrogenase activity in vivo.
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| Title: | 13C magnetic resonance spectroscopic imaging of hyperpolarized [1-13C, U-2H |
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| Authors: | Dzien, Piotr1,2, Kettunen, Mikko I.1,2, Marco‐Rius, Irene1, Serrao, Eva M.1,2, Rodrigues, Tiago B.1,2, Larkin, Timothy J.1, Timm, Kerstin N.1, Brindle, Kevin M.1,2 |
| Source: | Magnetic Resonance in Medicine. May2015, Vol. 73 Issue 5, p1733-1740. 8p. |
| Abstract: | Purpose Aldehyde dehydrogenase (ALDH2) is an emerging drug target for the treatment of heart disease, cocaine and alcohol dependence, and conditions caused by genetic polymorphisms in ALDH2. Noninvasive measurement of ALDH2 activity in vivo could inform the development of these drugs and accelerate their translation to the clinic. Methods [1-13C, U-2H5] ethanol was hyperpolarized using dynamic nuclear polarization, injected into mice and its oxidation in the liver monitored using 13C MR spectroscopy and spectroscopic imaging. Results Oxidation of [1-13C, U-2H5] ethanol to [1-13C] acetate was observed. Saturation of the acetaldehyde resonance, which was below the level of detection in vivo, demonstrated that acetate was produced via acetaldehyde. Irreversible inhibition of ALDH2 activity with disulfiram resulted in a proportional decrease in the amplitude of the acetate resonance. Conclusion 13C magnetic resonance spectroscopy measurements of hyperpolarized [1-13C, U-2H5] ethanol oxidation allow real-time assessment of ALDH2 activity in liver in vivo. Magn Reson Med, 2014. © The Authors. Magnetic Resonance in Medicine Published by Wiley Periodicals, Inc. on behalf of International Society of Medicine in Resonance. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited. Magn Reson Med 73:1733-1740, 2015. © 2014 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: <superscript>13</superscript>C magnetic resonance spectroscopic imaging of hyperpolarized [1-<superscript>13</superscript>C, U-<superscript>2</superscript>H<subscript>5</subscript>] ethanol oxidation can be used to assess aldehyde dehydrogenase activity in vivo. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dzien%2C+Piotr%22">Dzien, Piotr</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kettunen%2C+Mikko+I%2E%22">Kettunen, Mikko I.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Marco‐Rius%2C+Irene%22">Marco‐Rius, Irene</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Serrao%2C+Eva+M%2E%22">Serrao, Eva M.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Rodrigues%2C+Tiago+B%2E%22">Rodrigues, Tiago B.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Larkin%2C+Timothy+J%2E%22">Larkin, Timothy J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Timm%2C+Kerstin+N%2E%22">Timm, Kerstin N.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Brindle%2C+Kevin+M%2E%22">Brindle, Kevin M.</searchLink><relatesTo>1,2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. May2015, Vol. 73 Issue 5, p1733-1740. 8p. – Name: Abstract Label: Abstract Group: Ab Data: Purpose Aldehyde dehydrogenase (ALDH2) is an emerging drug target for the treatment of heart disease, cocaine and alcohol dependence, and conditions caused by genetic polymorphisms in ALDH2. Noninvasive measurement of ALDH2 activity in vivo could inform the development of these drugs and accelerate their translation to the clinic. Methods [1-13C, U-2H5] ethanol was hyperpolarized using dynamic nuclear polarization, injected into mice and its oxidation in the liver monitored using 13C MR spectroscopy and spectroscopic imaging. Results Oxidation of [1-13C, U-2H5] ethanol to [1-13C] acetate was observed. Saturation of the acetaldehyde resonance, which was below the level of detection in vivo, demonstrated that acetate was produced via acetaldehyde. Irreversible inhibition of ALDH2 activity with disulfiram resulted in a proportional decrease in the amplitude of the acetate resonance. Conclusion 13C magnetic resonance spectroscopy measurements of hyperpolarized [1-13C, U-2H5] ethanol oxidation allow real-time assessment of ALDH2 activity in liver in vivo. Magn Reson Med, 2014. © The Authors. Magnetic Resonance in Medicine Published by Wiley Periodicals, Inc. on behalf of International Society of Medicine in Resonance. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited. Magn Reson Med 73:1733-1740, 2015. © 2014 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.25286 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 1733 Titles: – TitleFull: 13C magnetic resonance spectroscopic imaging of hyperpolarized [1-13C, U-2H5] ethanol oxidation can be used to assess aldehyde dehydrogenase activity in vivo. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dzien, Piotr – PersonEntity: Name: NameFull: Kettunen, Mikko I. – PersonEntity: Name: NameFull: Marco‐Rius, Irene – PersonEntity: Name: NameFull: Serrao, Eva M. – PersonEntity: Name: NameFull: Rodrigues, Tiago B. – PersonEntity: Name: NameFull: Larkin, Timothy J. – PersonEntity: Name: NameFull: Timm, Kerstin N. – PersonEntity: Name: NameFull: Brindle, Kevin M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 73 – Type: issue Value: 5 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
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