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p47phox-Nox2-dependent ROS Signaling Inhibits Early Bone Development in Mice but Protects against Skeletal Aging.

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Title: p47phox-Nox2-dependent ROS Signaling Inhibits Early Bone Development in Mice but Protects against Skeletal Aging.
Authors: Jin-Ran Chen1,2 chenjinran@uams.edu, Lazarenko, Oxana P.1,2, Blackburn, Michael L.1,2, Mercer, Kelly E.1, Badger, Thomas M.1,2, Ronis, Martin J. J.1,2
Source: Journal of Biological Chemistry. 6/5/2015, Vol. 290 Issue 23, p14692-14704. 13p.
Subjects: Bone metabolism, NADPH oxidase, Reactive oxygen species, Cellular signal transduction, Physiological aspects of aging, Laboratory mice
Abstract: Bone remodeling is age-dependently regulated and changes dramatically during the course of development. Progressive accumulation of reactive oxygen species (ROS) has been suspected to be the leading cause of many inflammatory and degenerative diseases, as well as an important factor underlying many effects of aging. In contrast, how reduced ROS signaling regulates inflammation and remodeling in bone remains unknown. Here, we utilized a p47phox knockout mouse model, in which an essential cytosolic co-activator of Nox2 is lost, to characterize bone metabolism at 6 wks and 2 yrs of age. Compared to their age-matched wild type controls, loss of Nox2 function in p47phox -/- mice resulted in age-related switch of bone mass and strength. Differences in bone mass were associated with increased bone formation in 6 wks old p47phox -/- mice, but decreased in 2 yrs old p47phox -/- mice. Despite decreases in ROS generation in bone marrow cells and p47phox/Nox2 signaling in osteoblastic cells, 2 yrs old p47phox -/- mice showed increased senescence-associated secretory phenotype in bone compared to their wild type controls. These in vivo findings were mechanistically recapitulated in ex vivo cell culture of primary fetal calvarial cells from p47phox -/- mice. These cells showed accelerated cell senescence pathway accompanied by increased inflammation. These data indicate that the observed age-related switch of bone mass in p47phox deficient mice occurs through an increased inflammatory milieu in bone and that p47phox/Nox2-dependent physiological ROS signaling suppresses inflammation in aging. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
  Group: Ti
  Data: p47<superscript>phox</superscript>-Nox2-dependent ROS Signaling Inhibits Early Bone Development in Mice but Protects against Skeletal Aging.
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  Data: <searchLink fieldCode="AR" term="%22Jin-Ran+Chen%22">Jin-Ran Chen</searchLink><relatesTo>1,2</relatesTo><i> chenjinran@uams.edu</i><br /><searchLink fieldCode="AR" term="%22Lazarenko%2C+Oxana+P%2E%22">Lazarenko, Oxana P.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Blackburn%2C+Michael+L%2E%22">Blackburn, Michael L.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mercer%2C+Kelly+E%2E%22">Mercer, Kelly E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Badger%2C+Thomas+M%2E%22">Badger, Thomas M.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ronis%2C+Martin+J%2E+J%2E%22">Ronis, Martin J. J.</searchLink><relatesTo>1,2</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 6/5/2015, Vol. 290 Issue 23, p14692-14704. 13p.
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  Data: <searchLink fieldCode="DE" term="%22Bone+metabolism%22">Bone metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22NADPH+oxidase%22">NADPH oxidase</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Physiological+aspects+of+aging%22">Physiological aspects of aging</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+mice%22">Laboratory mice</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Bone remodeling is age-dependently regulated and changes dramatically during the course of development. Progressive accumulation of reactive oxygen species (ROS) has been suspected to be the leading cause of many inflammatory and degenerative diseases, as well as an important factor underlying many effects of aging. In contrast, how reduced ROS signaling regulates inflammation and remodeling in bone remains unknown. Here, we utilized a p47phox knockout mouse model, in which an essential cytosolic co-activator of Nox2 is lost, to characterize bone metabolism at 6 wks and 2 yrs of age. Compared to their age-matched wild type controls, loss of Nox2 function in p47phox -/- mice resulted in age-related switch of bone mass and strength. Differences in bone mass were associated with increased bone formation in 6 wks old p47phox -/- mice, but decreased in 2 yrs old p47phox -/- mice. Despite decreases in ROS generation in bone marrow cells and p47phox/Nox2 signaling in osteoblastic cells, 2 yrs old p47phox -/- mice showed increased senescence-associated secretory phenotype in bone compared to their wild type controls. These in vivo findings were mechanistically recapitulated in ex vivo cell culture of primary fetal calvarial cells from p47phox -/- mice. These cells showed accelerated cell senescence pathway accompanied by increased inflammation. These data indicate that the observed age-related switch of bone mass in p47phox deficient mice occurs through an increased inflammatory milieu in bone and that p47phox/Nox2-dependent physiological ROS signaling suppresses inflammation in aging. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1074/jbc.M114.633461
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        Text: English
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      – SubjectFull: Bone metabolism
        Type: general
      – SubjectFull: NADPH oxidase
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      – SubjectFull: Reactive oxygen species
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      – SubjectFull: Cellular signal transduction
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      – SubjectFull: Laboratory mice
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      – TitleFull: p47phox-Nox2-dependent ROS Signaling Inhibits Early Bone Development in Mice but Protects against Skeletal Aging.
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              Text: 6/5/2015
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