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Development and Validation of a Simple Reversed-Phase HPLC-UV Method for Determination of Malondialdehyde in Olive Oil.

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Bibliographic Details
Title: Development and Validation of a Simple Reversed-Phase HPLC-UV Method for Determination of Malondialdehyde in Olive Oil.
Authors: Al‐Rimawi, Fuad1 falrimawi@science.alquds.edu
Source: Journal of the American Oil Chemists' Society (JAOCS). Jul2015, Vol. 92 Issue 7, p933-937. 5p.
Subjects: High performance liquid chromatography, Ultraviolet radiation, Malondialdehyde, Olive oil analysis, Separation (Technology)
Abstract: A simple, precise, accurate and selective method was developed and validated for determination of malondialdehyde (MDA) in olive oil. Separation was achieved on a reversed-phase C column using a mobile phase consisting of methanol/0.8 % phosphoric acid (10:90, v/v), at a flow rate of 1.0 ml/min and UV detection at 220 nm. This method was validated according to the requirements for new methods, which include accuracy, precision, selectivity, robustness, a limit of detection, limit of quantitation (LOQ), linearity and range. The current method demonstrated good linearity over the range of 0.5-1000 ppm of MDA with r greater than 0.999. The recovery of MDA in olive oil ranged from 97.1 to 99.1 %. The method was selective where MDA was distinctly separated from other compounds of the oil with good resolution. The method was also precise where the RSD of the peak areas of replicate injections of MDA standard solution were less than 1 %. The degree of reproducibility of the results obtained as a result of small deliberate variations in the method parameters and by changing the analytical operators proved that the method is robust and rugged. The low LOQ of MDA (0.5 ppm) using this method enables quantitation of MDA at low concentration. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:A simple, precise, accurate and selective method was developed and validated for determination of malondialdehyde (MDA) in olive oil. Separation was achieved on a reversed-phase C column using a mobile phase consisting of methanol/0.8 % phosphoric acid (10:90, v/v), at a flow rate of 1.0 ml/min and UV detection at 220 nm. This method was validated according to the requirements for new methods, which include accuracy, precision, selectivity, robustness, a limit of detection, limit of quantitation (LOQ), linearity and range. The current method demonstrated good linearity over the range of 0.5-1000 ppm of MDA with r greater than 0.999. The recovery of MDA in olive oil ranged from 97.1 to 99.1 %. The method was selective where MDA was distinctly separated from other compounds of the oil with good resolution. The method was also precise where the RSD of the peak areas of replicate injections of MDA standard solution were less than 1 %. The degree of reproducibility of the results obtained as a result of small deliberate variations in the method parameters and by changing the analytical operators proved that the method is robust and rugged. The low LOQ of MDA (0.5 ppm) using this method enables quantitation of MDA at low concentration. [ABSTRACT FROM AUTHOR]
ISSN:0003021X
DOI:10.1007/s11746-015-2664-x