Effect of albumin and dextrose concentration on ultrasound and microbubble mediated gene transfection in vivo.
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| Title: | Effect of albumin and dextrose concentration on ultrasound and microbubble mediated gene transfection in vivo. |
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| Authors: | Browning RJ (AUTHOR), Mulvana H (AUTHOR), Tang MX (AUTHOR), Hajnal JV (AUTHOR), Wells DJ (AUTHOR), Eckersley RJ (AUTHOR), Browning, Richard J1 (AUTHOR), Mulvana, Helen (AUTHOR), Tang, Meng-Xing (AUTHOR), Hajnal, Jo V (AUTHOR), Wells, Dominic J (AUTHOR), Eckersley, Robert J (AUTHOR) |
| Source: | Ultrasound in Medicine & Biology. Jun2012, Vol. 38 Issue 6, p1067-1077. 11p. |
| Abstract: | Ultrasound and microbubble mediated gene transfection has great potential for site-selective, safe gene delivery. Albumin-based microbubbles have shown the greatest transfection efficiency but have not been optimised specifically for this purpose. Additionally, few studies have highlighted desirable properties for transfection specific microbubbles. In this article, microbubbles were made with 2% or 5% (w/v) albumin and 20% or 40% (w/v) dextrose solutions, yielding four distinct bubble types. These were acoustically characterised and their efficiency in transfecting a luciferase plasmid (pGL4.13) into female, CD1 mice myocardia was measured. For either albumin concentration, increasing the dextrose concentration increased scattering, attenuation and resistance to ultrasound, resulting in significantly increased transfection. A significant interaction was noted between albumin and dextrose; 2% albumin bubbles made with 20% dextrose showed the least transfection but the most transfection with 40% dextrose. This trend was seen for both nonlinear scattering and attenuation behaviour but not for resistance to ultrasound or total scatter. We have determined that the attenuation behaviour is an important microbubble characteristic for effective gene transfection using ultrasound. Microbubble behaviour can also be simply controlled by altering the initial ingredients used during manufacture. [ABSTRACT FROM AUTHOR] |
| Copyright of Ultrasound in Medicine & Biology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 104435830 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Effect of albumin and dextrose concentration on ultrasound and microbubble mediated gene transfection in vivo. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Browning+RJ%22">Browning RJ</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mulvana+H%22">Mulvana H</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tang+MX%22">Tang MX</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hajnal+JV%22">Hajnal JV</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wells+DJ%22">Wells DJ</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Eckersley+RJ%22">Eckersley RJ</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Browning%2C+Richard+J%22">Browning, Richard J</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mulvana%2C+Helen%22">Mulvana, Helen</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tang%2C+Meng-Xing%22">Tang, Meng-Xing</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hajnal%2C+Jo+V%22">Hajnal, Jo V</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wells%2C+Dominic+J%22">Wells, Dominic J</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Eckersley%2C+Robert+J%22">Eckersley, Robert J</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Ultrasound+in+Medicine+%26+Biology%22">Ultrasound in Medicine & Biology</searchLink>. Jun2012, Vol. 38 Issue 6, p1067-1077. 11p. – Name: Abstract Label: Abstract Group: Ab Data: Ultrasound and microbubble mediated gene transfection has great potential for site-selective, safe gene delivery. Albumin-based microbubbles have shown the greatest transfection efficiency but have not been optimised specifically for this purpose. Additionally, few studies have highlighted desirable properties for transfection specific microbubbles. In this article, microbubbles were made with 2% or 5% (w/v) albumin and 20% or 40% (w/v) dextrose solutions, yielding four distinct bubble types. These were acoustically characterised and their efficiency in transfecting a luciferase plasmid (pGL4.13) into female, CD1 mice myocardia was measured. For either albumin concentration, increasing the dextrose concentration increased scattering, attenuation and resistance to ultrasound, resulting in significantly increased transfection. A significant interaction was noted between albumin and dextrose; 2% albumin bubbles made with 20% dextrose showed the least transfection but the most transfection with 40% dextrose. This trend was seen for both nonlinear scattering and attenuation behaviour but not for resistance to ultrasound or total scatter. We have determined that the attenuation behaviour is an important microbubble characteristic for effective gene transfection using ultrasound. Microbubble behaviour can also be simply controlled by altering the initial ingredients used during manufacture. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Ultrasound in Medicine & Biology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.ultrasmedbio.2012.02.020 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1067 Titles: – TitleFull: Effect of albumin and dextrose concentration on ultrasound and microbubble mediated gene transfection in vivo. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Browning RJ – PersonEntity: Name: NameFull: Mulvana H – PersonEntity: Name: NameFull: Tang MX – PersonEntity: Name: NameFull: Hajnal JV – PersonEntity: Name: NameFull: Wells DJ – PersonEntity: Name: NameFull: Eckersley RJ – PersonEntity: Name: NameFull: Browning, Richard J – PersonEntity: Name: NameFull: Mulvana, Helen – PersonEntity: Name: NameFull: Tang, Meng-Xing – PersonEntity: Name: NameFull: Hajnal, Jo V – PersonEntity: Name: NameFull: Wells, Dominic J – PersonEntity: Name: NameFull: Eckersley, Robert J IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2012 Type: published Y: 2012 Identifiers: – Type: issn-print Value: 03015629 Numbering: – Type: volume Value: 38 – Type: issue Value: 6 Titles: – TitleFull: Ultrasound in Medicine & Biology Type: main |
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