View in EDS HTML Full Text PDF Full Text

MR Imaging of activated hepatic stellate cells in liver injured by CCl4 of rats with integrin-targeted ultrasmall superparamagnetic iron oxide.

Saved in:
Bibliographic Details
Title: MR Imaging of activated hepatic stellate cells in liver injured by CCl4 of rats with integrin-targeted ultrasmall superparamagnetic iron oxide.
Authors: Wang QB (AUTHOR), Han Y (AUTHOR), Jiang TT (AUTHOR), Chai WM (AUTHOR), Chen KM (AUTHOR), Liu BY (AUTHOR), Wang LF (AUTHOR), Zhang C (AUTHOR), Wang DB (AUTHOR), Wang, Qing-Bing1 (AUTHOR), Han, Yu (AUTHOR), Jiang, Ting-Ting (AUTHOR), Chai, Wei-Min (AUTHOR), Chen, Ke-Min (AUTHOR), Liu, Bing-Ya (AUTHOR), Wang, Li-Fu (AUTHOR), Zhang, Chunfu (AUTHOR), Wang, Deng-Bin (AUTHOR)
Source: European Radiology. May2011, Vol. 21 Issue 5, p1016-1025. 10p.
Abstract: Objective: To demonstrate the feasibility of the ultrasmall superparamagnetic iron oxide (USPIO) modified by cyclo (Arg-Gly-Asp-Try-Cys) peptide (c(RGDyC)-USPIO) for targeting hepatic stellate cells (HSCs).Materials and Methods: A c(RGDyC)-USPIO probe was prepared by conjugating c(RGDyC) with USPIO through a thiol-maleinide interaction. The specificity of c(RGDyC)-USPIO for HSCs was investigated in vitro. In vivo, normal and fibrosis rats were treated with either c(RGDyC)-USPIO or USPIO, and magnetic resonance imaging (MRI) of the rats performed after administration of the probes for 4 h. The T2 relaxation times changes before and after probe injection were analyzed and the locations of probes in normal or injured mice were identified histologically.Results: The hydrodynamic size of c(RGDyC)-USPIO was 13 ± 3 nm. HSCs took up more specific probes than plain ones. The reduction of T2 relaxation times in fibrosis rat by c(RGDyC)-USPIO was much greater than that by USPIO (P < 0.05). Prussian blue staining and transmission electron microscopy of the injured rat liver treated with c(RGDyC) demonstrated that c(RGDyC)-USPIO were specifically engulfed by the activated HSCs.Conclusion: In vivo cellular targeted imaging of activated HSCs in liver fibrosis using c(RGDyC)-USPIO targeting α(v)β(3) integrins was feasible using a clinical 1.5-Tesla MR system. [ABSTRACT FROM AUTHOR]
Copyright of European Radiology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 104866638
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: MR Imaging of activated hepatic stellate cells in liver injured by CCl4 of rats with integrin-targeted ultrasmall superparamagnetic iron oxide.
– Name: Author
  Label: Authors
  Group: Au
  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang+QB%22&quot;&gt;Wang QB&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Han+Y%22&quot;&gt;Han Y&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Jiang+TT%22&quot;&gt;Jiang TT&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Chai+WM%22&quot;&gt;Chai WM&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Chen+KM%22&quot;&gt;Chen KM&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Liu+BY%22&quot;&gt;Liu BY&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang+LF%22&quot;&gt;Wang LF&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhang+C%22&quot;&gt;Zhang C&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang+DB%22&quot;&gt;Wang DB&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang%2C+Qing-Bing%22&quot;&gt;Wang, Qing-Bing&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Han%2C+Yu%22&quot;&gt;Han, Yu&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Jiang%2C+Ting-Ting%22&quot;&gt;Jiang, Ting-Ting&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Chai%2C+Wei-Min%22&quot;&gt;Chai, Wei-Min&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Chen%2C+Ke-Min%22&quot;&gt;Chen, Ke-Min&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Liu%2C+Bing-Ya%22&quot;&gt;Liu, Bing-Ya&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang%2C+Li-Fu%22&quot;&gt;Wang, Li-Fu&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhang%2C+Chunfu%22&quot;&gt;Zhang, Chunfu&lt;/searchLink&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang%2C+Deng-Bin%22&quot;&gt;Wang, Deng-Bin&lt;/searchLink&gt; (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22European+Radiology%22&quot;&gt;European Radiology&lt;/searchLink&gt;. May2011, Vol. 21 Issue 5, p1016-1025. 10p.
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: &lt;bold&gt;Objective: &lt;/bold&gt;To demonstrate the feasibility of the ultrasmall superparamagnetic iron oxide (USPIO) modified by cyclo (Arg-Gly-Asp-Try-Cys) peptide (c(RGDyC)-USPIO) for targeting hepatic stellate cells (HSCs).&lt;bold&gt;Materials and Methods: &lt;/bold&gt;A c(RGDyC)-USPIO probe was prepared by conjugating c(RGDyC) with USPIO through a thiol-maleinide interaction. The specificity of c(RGDyC)-USPIO for HSCs was investigated in vitro. In vivo, normal and fibrosis rats were treated with either c(RGDyC)-USPIO or USPIO, and magnetic resonance imaging (MRI) of the rats performed after administration of the probes for 4 h. The T2 relaxation times changes before and after probe injection were analyzed and the locations of probes in normal or injured mice were identified histologically.&lt;bold&gt;Results: &lt;/bold&gt;The hydrodynamic size of c(RGDyC)-USPIO was 13 &#177; 3 nm. HSCs took up more specific probes than plain ones. The reduction of T2 relaxation times in fibrosis rat by c(RGDyC)-USPIO was much greater than that by USPIO (P &lt; 0.05). Prussian blue staining and transmission electron microscopy of the injured rat liver treated with c(RGDyC) demonstrated that c(RGDyC)-USPIO were specifically engulfed by the activated HSCs.&lt;bold&gt;Conclusion: &lt;/bold&gt;In vivo cellular targeted imaging of activated HSCs in liver fibrosis using c(RGDyC)-USPIO targeting α(v)β(3) integrins was feasible using a clinical 1.5-Tesla MR system. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: &lt;i&gt;Copyright of European Radiology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=104866638
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s00330-010-1988-z
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 1016
    Titles:
      – TitleFull: MR Imaging of activated hepatic stellate cells in liver injured by CCl4 of rats with integrin-targeted ultrasmall superparamagnetic iron oxide.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Wang QB
      – PersonEntity:
          Name:
            NameFull: Han Y
      – PersonEntity:
          Name:
            NameFull: Jiang TT
      – PersonEntity:
          Name:
            NameFull: Chai WM
      – PersonEntity:
          Name:
            NameFull: Chen KM
      – PersonEntity:
          Name:
            NameFull: Liu BY
      – PersonEntity:
          Name:
            NameFull: Wang LF
      – PersonEntity:
          Name:
            NameFull: Zhang C
      – PersonEntity:
          Name:
            NameFull: Wang DB
      – PersonEntity:
          Name:
            NameFull: Wang, Qing-Bing
      – PersonEntity:
          Name:
            NameFull: Han, Yu
      – PersonEntity:
          Name:
            NameFull: Jiang, Ting-Ting
      – PersonEntity:
          Name:
            NameFull: Chai, Wei-Min
      – PersonEntity:
          Name:
            NameFull: Chen, Ke-Min
      – PersonEntity:
          Name:
            NameFull: Liu, Bing-Ya
      – PersonEntity:
          Name:
            NameFull: Wang, Li-Fu
      – PersonEntity:
          Name:
            NameFull: Zhang, Chunfu
      – PersonEntity:
          Name:
            NameFull: Wang, Deng-Bin
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 05
              Text: May2011
              Type: published
              Y: 2011
          Identifiers:
            – Type: issn-print
              Value: 09387994
          Numbering:
            – Type: volume
              Value: 21
            – Type: issue
              Value: 5
          Titles:
            – TitleFull: European Radiology
              Type: main
ResultId 1