The Intestinal Microbiota Influences Campylobacter jejuni Colonization and Extraintestinal Dissemination in Mice.
Saved in:
| Title: | The Intestinal Microbiota Influences Campylobacter jejuni Colonization and Extraintestinal Dissemination in Mice. |
|---|---|
| Authors: | O'Loughlin, Jason L.1, Samuelson, Derrick R.1, Braundmeier-Fleming, Andrea G.2,3, White, Bryan A.3, Haldorson, Gary J.4, Stone, Jennifer B.1, Lessmann, Jeremy J.5, Eucker, Tyson P.1, Konkel, Michael E.1 konkel@vetmed.wsu.edu |
| Source: | Applied & Environmental Microbiology. Jul2015, Vol. 81 Issue 14, p4642-4650. 9p. |
| Subjects: | Campylobacter jejuni, Foodborne diseases, Gastroenteritis, Viral gastroenteritis, Ampicillin, Infectious disease transmission, Therapeutics |
| Abstract: | Campylobacter jejuni is a leading cause of human foodborne gastroenteritis worldwide. The interactions between this pathogen and the intestinal microbiome within a host are of interest as endogenous intestinal microbiota mediates a form of resistance to the pathogen. This resistance, termed colonization resistance, is the ability of commensal microbiota to prevent colonization by exogenous pathogens or opportunistic commensals. Although mice normally demonstrate colonization resistance to C. jejuni, we found that mice treated with ampicillin are colonized by C. jejuni, with recovery of Campylobacter from the colon, mesenteric lymph nodes, and spleen. Furthermore, there was a significant reduction in recovery of C. jejuni from ampicillin-treated mice inoculated with a C. jejuni virulence mutant (ΔflgL strain) compared to recovery of mice inoculated with the C. jejuni wildtype strain or the C. jejuni complemented isolate (ΔflgL/flgL). Comparative analysis of the microbiota from nontreated and ampicillin-treated CBA/J mice led to the identification of a lactic acid-fermenting isolate of Enterococcus faecalis that prevented C. jejuni growth in vitro and limited C. jejuni colonization of mice. Next-generation sequencing of DNA from fecal pellets that were collected from ampicillin-treated CBA/J mice revealed a significant decrease in diversity of operational taxonomic units (OTUs) compared to that in control (nontreated) mice. Taken together, we have demonstrated that treatment of mice with ampicillin alters the intestinal microbiota and permits C. jejuni colonization. These findings provide valuable insights for researchers using mice to investigate C. jejuni colonization factors, virulence determinants, or the mechanistic basis of probiotics. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 108471378 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: The Intestinal Microbiota Influences Campylobacter jejuni Colonization and Extraintestinal Dissemination in Mice. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22O'Loughlin%2C+Jason+L%2E%22">O'Loughlin, Jason L.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Samuelson%2C+Derrick+R%2E%22">Samuelson, Derrick R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Braundmeier-Fleming%2C+Andrea+G%2E%22">Braundmeier-Fleming, Andrea G.</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22White%2C+Bryan+A%2E%22">White, Bryan A.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Haldorson%2C+Gary+J%2E%22">Haldorson, Gary J.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Stone%2C+Jennifer+B%2E%22">Stone, Jennifer B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lessmann%2C+Jeremy+J%2E%22">Lessmann, Jeremy J.</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Eucker%2C+Tyson+P%2E%22">Eucker, Tyson P.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Konkel%2C+Michael+E%2E%22">Konkel, Michael E.</searchLink><relatesTo>1</relatesTo><i> konkel@vetmed.wsu.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+%26+Environmental+Microbiology%22">Applied & Environmental Microbiology</searchLink>. Jul2015, Vol. 81 Issue 14, p4642-4650. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Campylobacter+jejuni%22">Campylobacter jejuni</searchLink><br /><searchLink fieldCode="DE" term="%22Foodborne+diseases%22">Foodborne diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Gastroenteritis%22">Gastroenteritis</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+gastroenteritis%22">Viral gastroenteritis</searchLink><br /><searchLink fieldCode="DE" term="%22Ampicillin%22">Ampicillin</searchLink><br /><searchLink fieldCode="DE" term="%22Infectious+disease+transmission%22">Infectious disease transmission</searchLink><br /><searchLink fieldCode="DE" term="%22Therapeutics%22">Therapeutics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Campylobacter jejuni is a leading cause of human foodborne gastroenteritis worldwide. The interactions between this pathogen and the intestinal microbiome within a host are of interest as endogenous intestinal microbiota mediates a form of resistance to the pathogen. This resistance, termed colonization resistance, is the ability of commensal microbiota to prevent colonization by exogenous pathogens or opportunistic commensals. Although mice normally demonstrate colonization resistance to C. jejuni, we found that mice treated with ampicillin are colonized by C. jejuni, with recovery of Campylobacter from the colon, mesenteric lymph nodes, and spleen. Furthermore, there was a significant reduction in recovery of C. jejuni from ampicillin-treated mice inoculated with a C. jejuni virulence mutant (ΔflgL strain) compared to recovery of mice inoculated with the C. jejuni wildtype strain or the C. jejuni complemented isolate (ΔflgL/flgL). Comparative analysis of the microbiota from nontreated and ampicillin-treated CBA/J mice led to the identification of a lactic acid-fermenting isolate of Enterococcus faecalis that prevented C. jejuni growth in vitro and limited C. jejuni colonization of mice. Next-generation sequencing of DNA from fecal pellets that were collected from ampicillin-treated CBA/J mice revealed a significant decrease in diversity of operational taxonomic units (OTUs) compared to that in control (nontreated) mice. Taken together, we have demonstrated that treatment of mice with ampicillin alters the intestinal microbiota and permits C. jejuni colonization. These findings provide valuable insights for researchers using mice to investigate C. jejuni colonization factors, virulence determinants, or the mechanistic basis of probiotics. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=108471378 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1128/AEM.00281-15 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 4642 Subjects: – SubjectFull: Campylobacter jejuni Type: general – SubjectFull: Foodborne diseases Type: general – SubjectFull: Gastroenteritis Type: general – SubjectFull: Viral gastroenteritis Type: general – SubjectFull: Ampicillin Type: general – SubjectFull: Infectious disease transmission Type: general – SubjectFull: Therapeutics Type: general Titles: – TitleFull: The Intestinal Microbiota Influences Campylobacter jejuni Colonization and Extraintestinal Dissemination in Mice. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: O'Loughlin, Jason L. – PersonEntity: Name: NameFull: Samuelson, Derrick R. – PersonEntity: Name: NameFull: Braundmeier-Fleming, Andrea G. – PersonEntity: Name: NameFull: White, Bryan A. – PersonEntity: Name: NameFull: Haldorson, Gary J. – PersonEntity: Name: NameFull: Stone, Jennifer B. – PersonEntity: Name: NameFull: Lessmann, Jeremy J. – PersonEntity: Name: NameFull: Eucker, Tyson P. – PersonEntity: Name: NameFull: Konkel, Michael E. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 07 Text: Jul2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 00992240 Numbering: – Type: volume Value: 81 – Type: issue Value: 14 Titles: – TitleFull: Applied & Environmental Microbiology Type: main |
| ResultId | 1 |
