Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411.
Saved in:
| Title: | Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411. |
|---|---|
| Authors: | Drosg, B.1, Fritz, I.1 ines.fritz@boku.ac.at, Gattermayr, F.1, Silvestrini, L.1 |
| Source: | Chemical & Biochemical Engineering Quarterly. Jul2015, Vol. 29 Issue 2, p87-98. 12p. |
| Subjects: | Microbial polymers, Autotrophic bacteria, Cyanobacteria, Heterotrophic bacteria, Polyesters, Flue gases |
| Abstract: | The halobacterium Haloferax mediterranei was used to study the production of two types of biopolymers: The biopolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was accumulated as intracellular granules, whereas an extracelluar polysaccharide was excreted in parallel to biopolyester synthesis. After production, microbial re-use and degradation of these polymers under different conditions were investigated to assess the requirements for handling the product-rich fermentation broth prior to the downstream processing for product recovery. Degradation kinetics of the polymers and the impact of different storage conditions on molar mass of PHBV were studied. It turned out that the biotechnological fermentation process can be run without any sterility precautions. No major product losses were observed without pasteurization of fermentation broth after the stop of fermentation. In addition, neither PHBV nor EPS are re-utilized by the cells for biomass formation even if the culture is maintained under conditions of carbon starvation for an extended time. [ABSTRACT FROM AUTHOR] |
| Copyright of Chemical & Biochemical Engineering Quarterly is the property of Croatian Society of Chemical Engineers and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 108615471 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Drosg%2C+B%2E%22">Drosg, B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fritz%2C+I%2E%22">Fritz, I.</searchLink><relatesTo>1</relatesTo><i> ines.fritz@boku.ac.at</i><br /><searchLink fieldCode="AR" term="%22Gattermayr%2C+F%2E%22">Gattermayr, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Silvestrini%2C+L%2E%22">Silvestrini, L.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Chemical+%26+Biochemical+Engineering+Quarterly%22">Chemical & Biochemical Engineering Quarterly</searchLink>. Jul2015, Vol. 29 Issue 2, p87-98. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Microbial+polymers%22">Microbial polymers</searchLink><br /><searchLink fieldCode="DE" term="%22Autotrophic+bacteria%22">Autotrophic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Cyanobacteria%22">Cyanobacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Heterotrophic+bacteria%22">Heterotrophic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Polyesters%22">Polyesters</searchLink><br /><searchLink fieldCode="DE" term="%22Flue+gases%22">Flue gases</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The halobacterium Haloferax mediterranei was used to study the production of two types of biopolymers: The biopolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was accumulated as intracellular granules, whereas an extracelluar polysaccharide was excreted in parallel to biopolyester synthesis. After production, microbial re-use and degradation of these polymers under different conditions were investigated to assess the requirements for handling the product-rich fermentation broth prior to the downstream processing for product recovery. Degradation kinetics of the polymers and the impact of different storage conditions on molar mass of PHBV were studied. It turned out that the biotechnological fermentation process can be run without any sterility precautions. No major product losses were observed without pasteurization of fermentation broth after the stop of fermentation. In addition, neither PHBV nor EPS are re-utilized by the cells for biomass formation even if the culture is maintained under conditions of carbon starvation for an extended time. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Chemical & Biochemical Engineering Quarterly is the property of Croatian Society of Chemical Engineers and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=108615471 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.15255/CABEQ.2014.2058 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 87 Subjects: – SubjectFull: Microbial polymers Type: general – SubjectFull: Autotrophic bacteria Type: general – SubjectFull: Cyanobacteria Type: general – SubjectFull: Heterotrophic bacteria Type: general – SubjectFull: Polyesters Type: general – SubjectFull: Flue gases Type: general Titles: – TitleFull: Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Drosg, B. – PersonEntity: Name: NameFull: Fritz, I. – PersonEntity: Name: NameFull: Gattermayr, F. – PersonEntity: Name: NameFull: Silvestrini, L. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 03529568 Numbering: – Type: volume Value: 29 – Type: issue Value: 2 Titles: – TitleFull: Chemical & Biochemical Engineering Quarterly Type: main |
| ResultId | 1 |
