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Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains.

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Title: Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains.
Authors: Follonier, S.1, Riesen, R.2, Zinn, M.1 manfred.zinn@hevs.ch
Source: Chemical & Biochemical Engineering Quarterly. Jul2015, Vol. 29 Issue 2, p99-112. 14p.
Subjects: Polyesters, Microbial polymers, Grapes, Fatty acids, Biosynthesis, Pseudomonas putida
Abstract: Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 sugars and 0.7 g L-1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L-1 with a PHB content of 66% in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L-1 dry biomass containing 46% PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L-1 of biomass containing 78% PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production. [ABSTRACT FROM AUTHOR]
Copyright of Chemical & Biochemical Engineering Quarterly is the property of Croatian Society of Chemical Engineers and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains.
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  Data: <searchLink fieldCode="AR" term="%22Follonier%2C+S%2E%22">Follonier, S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Riesen%2C+R%2E%22">Riesen, R.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zinn%2C+M%2E%22">Zinn, M.</searchLink><relatesTo>1</relatesTo><i> manfred.zinn@hevs.ch</i>
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  Data: <searchLink fieldCode="JN" term="%22Chemical+%26+Biochemical+Engineering+Quarterly%22">Chemical & Biochemical Engineering Quarterly</searchLink>. Jul2015, Vol. 29 Issue 2, p99-112. 14p.
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  Data: <searchLink fieldCode="DE" term="%22Polyesters%22">Polyesters</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+polymers%22">Microbial polymers</searchLink><br /><searchLink fieldCode="DE" term="%22Grapes%22">Grapes</searchLink><br /><searchLink fieldCode="DE" term="%22Fatty+acids%22">Fatty acids</searchLink><br /><searchLink fieldCode="DE" term="%22Biosynthesis%22">Biosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonas+putida%22">Pseudomonas putida</searchLink>
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  Data: Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 sugars and 0.7 g L-1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L-1 with a PHB content of 66% in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L-1 dry biomass containing 46% PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L-1 of biomass containing 78% PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Chemical & Biochemical Engineering Quarterly is the property of Croatian Society of Chemical Engineers and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.15255/CABEQ.2014.2250
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      – SubjectFull: Grapes
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      – SubjectFull: Pseudomonas putida
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      – TitleFull: Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains.
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              Text: Jul2015
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