Toxicity response of a new enzyme-based functional diversity methodology for Zn-contaminated field-collected soils.
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| Title: | Toxicity response of a new enzyme-based functional diversity methodology for Zn-contaminated field-collected soils. |
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| Authors: | Lessard, Isabelle1 isabelle.lessard@polymtl.ca, Sauvé, Sébastien2, Deschênes, Louise1 |
| Source: | Soil Biology & Biochemistry. Apr2014, p87-94. 8p. |
| Subjects: | Soil enzymology, Zinc toxicology, Microbial diversity, Enzymatic analysis, In situ remediation, Bioavailability |
| Abstract: | Assessing the effects of stressors, such as metals, on soils by microbial functional diversity (FD) indices based on soil enzymes is increasingly promoted. Various enzymatic FD indices were used in past studies but none generated toxicity thresholds such as EC50, the concentration of stressor affecting 50% of the maximum enzymatic activity. The aim of this study was to generate a realistic EC50 value from an enzyme-based FD index sensitive to Zn from long-term Zn-contaminated soils. Assays of 6 soil enzymes (arylsulfatase, phosphatase, protease, urease, β-glucosidase and invertase) and metal characterization (total, soluble and labile Zn) were measured in 10 pairs of Zn-contaminated field-collected soils. Two approaches were studied in parallel: enzymatic activity (EA) and enzymatic stability (ES) assessed by the RSSIb, a score based on the modified Relative Soil Stability Index (RSSI) method. The enzymatic results (activity and stability) were then aggregated by various FD indices (weighted and geometric means, Shannon, Simpson and Gini indices) and correlated to Zn concentrations in soils to obtain the EC50 of these concentration-response curves. The concentration-response curves constructed from the EA weighted means were highly correlated to the labile and soluble Zn concentrations, and the EC50 generated from these curves were in a realistic bioavailable Zn range as compared to other studies. The aggregated ES showed Zn-sensitivity similar to the aggregated EA. Therefore, although the use of the ES approach was judicious for long-term Zn contamination, the greater resources required to assess the ES by RSSIb scores does not seem justified. [ABSTRACT FROM AUTHOR] |
| Copyright of Soil Biology & Biochemistry is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 109254902 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Toxicity response of a new enzyme-based functional diversity methodology for Zn-contaminated field-collected soils. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lessard%2C+Isabelle%22">Lessard, Isabelle</searchLink><relatesTo>1</relatesTo><i> isabelle.lessard@polymtl.ca</i><br /><searchLink fieldCode="AR" term="%22Sauvé%2C+Sébastien%22">Sauvé, Sébastien</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Deschênes%2C+Louise%22">Deschênes, Louise</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Soil+Biology+%26+Biochemistry%22">Soil Biology & Biochemistry</searchLink>. Apr2014, p87-94. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Soil+enzymology%22">Soil enzymology</searchLink><br /><searchLink fieldCode="DE" term="%22Zinc+toxicology%22">Zinc toxicology</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+diversity%22">Microbial diversity</searchLink><br /><searchLink fieldCode="DE" term="%22Enzymatic+analysis%22">Enzymatic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22In+situ+remediation%22">In situ remediation</searchLink><br /><searchLink fieldCode="DE" term="%22Bioavailability%22">Bioavailability</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Assessing the effects of stressors, such as metals, on soils by microbial functional diversity (FD) indices based on soil enzymes is increasingly promoted. Various enzymatic FD indices were used in past studies but none generated toxicity thresholds such as EC50, the concentration of stressor affecting 50% of the maximum enzymatic activity. The aim of this study was to generate a realistic EC50 value from an enzyme-based FD index sensitive to Zn from long-term Zn-contaminated soils. Assays of 6 soil enzymes (arylsulfatase, phosphatase, protease, urease, β-glucosidase and invertase) and metal characterization (total, soluble and labile Zn) were measured in 10 pairs of Zn-contaminated field-collected soils. Two approaches were studied in parallel: enzymatic activity (EA) and enzymatic stability (ES) assessed by the RSSIb, a score based on the modified Relative Soil Stability Index (RSSI) method. The enzymatic results (activity and stability) were then aggregated by various FD indices (weighted and geometric means, Shannon, Simpson and Gini indices) and correlated to Zn concentrations in soils to obtain the EC50 of these concentration-response curves. The concentration-response curves constructed from the EA weighted means were highly correlated to the labile and soluble Zn concentrations, and the EC50 generated from these curves were in a realistic bioavailable Zn range as compared to other studies. The aggregated ES showed Zn-sensitivity similar to the aggregated EA. Therefore, although the use of the ES approach was judicious for long-term Zn contamination, the greater resources required to assess the ES by RSSIb scores does not seem justified. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Soil Biology & Biochemistry is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.soilbio.2014.01.002 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 87 Subjects: – SubjectFull: Soil enzymology Type: general – SubjectFull: Zinc toxicology Type: general – SubjectFull: Microbial diversity Type: general – SubjectFull: Enzymatic analysis Type: general – SubjectFull: In situ remediation Type: general – SubjectFull: Bioavailability Type: general Titles: – TitleFull: Toxicity response of a new enzyme-based functional diversity methodology for Zn-contaminated field-collected soils. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lessard, Isabelle – PersonEntity: Name: NameFull: Sauvé, Sébastien – PersonEntity: Name: NameFull: Deschênes, Louise IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 00380717 Titles: – TitleFull: Soil Biology & Biochemistry Type: main |
| ResultId | 1 |
