Metabolic engineering of Escherichia coli to enhance acetol production from glycerol.
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| Title: | Metabolic engineering of Escherichia coli to enhance acetol production from glycerol. |
|---|---|
| Authors: | Yao, Ruilian1, Liu, Qing1, Hu, Hongbo1 hbhu@sjtu.edu.cn, Wood, Thomas2, Zhang, Xuehong1 |
| Source: | Applied Microbiology & Biotechnology. Oct2015, Vol. 99 Issue 19, p7945-7952. 8p. |
| Subjects: | Escherichia coli, Hydroxypropanone, Glycerin, Polyols, Acrolein, Aldehyde dehydrogenase |
| Abstract: | Acetol, a C3 keto alcohol, is an important intermediate used to produce polyols and acrolein. To enhance acetol production from glycerol by Escherichia coli, a mutant (HJ02) was constructed by replacing the native glpK gene with the allele from E. coli Lin 43 and overexpression of yqhD, which encodes aldehyde oxidoreductase YqhD that converts methylglyoxal to acetol. Compared to the control strain without the glpK replacement, HJ02 had 5.5 times greater acetol production and a 53.4 % higher glycerol consumption rate. Then, glucose was added as a co-substrate to enhance NADPH availability and the ptsG gene was deleted in HJ02 (HJ04) to alleviate carbon catabolite repression, which led to a 30 % increase in the NADPH level and NADPH/NADP. Consequently, HJ04 accumulated up to 1.20 g/L of acetol, which is 69.0 % higher than that of HJ02. Furthermore, the gapA gene in HJ04 was silenced by antisense RNA (HJ05) to further enhance acetol production. The acetol concentration produced by HJ05 reached 1.82 g/L, which was 2.1 and 1.5 times higher than that of HJ02 and HJ04. Real-time PCR analysis indicates that glucose catabolism was rerouted from glycolysis to the oxidative pentose phosphate pathway in HJ05. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Metabolic engineering of Escherichia coli to enhance acetol production from glycerol. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Yao%2C+Ruilian%22">Yao, Ruilian</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Qing%22">Liu, Qing</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hu%2C+Hongbo%22">Hu, Hongbo</searchLink><relatesTo>1</relatesTo><i> hbhu@sjtu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Wood%2C+Thomas%22">Wood, Thomas</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Xuehong%22">Zhang, Xuehong</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Oct2015, Vol. 99 Issue 19, p7945-7952. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Hydroxypropanone%22">Hydroxypropanone</searchLink><br /><searchLink fieldCode="DE" term="%22Glycerin%22">Glycerin</searchLink><br /><searchLink fieldCode="DE" term="%22Polyols%22">Polyols</searchLink><br /><searchLink fieldCode="DE" term="%22Acrolein%22">Acrolein</searchLink><br /><searchLink fieldCode="DE" term="%22Aldehyde+dehydrogenase%22">Aldehyde dehydrogenase</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Acetol, a C3 keto alcohol, is an important intermediate used to produce polyols and acrolein. To enhance acetol production from glycerol by Escherichia coli, a mutant (HJ02) was constructed by replacing the native glpK gene with the allele from E. coli Lin 43 and overexpression of yqhD, which encodes aldehyde oxidoreductase YqhD that converts methylglyoxal to acetol. Compared to the control strain without the glpK replacement, HJ02 had 5.5 times greater acetol production and a 53.4 % higher glycerol consumption rate. Then, glucose was added as a co-substrate to enhance NADPH availability and the ptsG gene was deleted in HJ02 (HJ04) to alleviate carbon catabolite repression, which led to a 30 % increase in the NADPH level and NADPH/NADP. Consequently, HJ04 accumulated up to 1.20 g/L of acetol, which is 69.0 % higher than that of HJ02. Furthermore, the gapA gene in HJ04 was silenced by antisense RNA (HJ05) to further enhance acetol production. The acetol concentration produced by HJ05 reached 1.82 g/L, which was 2.1 and 1.5 times higher than that of HJ02 and HJ04. Real-time PCR analysis indicates that glucose catabolism was rerouted from glycolysis to the oxidative pentose phosphate pathway in HJ05. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-015-6732-9 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 7945 Subjects: – SubjectFull: Escherichia coli Type: general – SubjectFull: Hydroxypropanone Type: general – SubjectFull: Glycerin Type: general – SubjectFull: Polyols Type: general – SubjectFull: Acrolein Type: general – SubjectFull: Aldehyde dehydrogenase Type: general Titles: – TitleFull: Metabolic engineering of Escherichia coli to enhance acetol production from glycerol. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Yao, Ruilian – PersonEntity: Name: NameFull: Liu, Qing – PersonEntity: Name: NameFull: Hu, Hongbo – PersonEntity: Name: NameFull: Wood, Thomas – PersonEntity: Name: NameFull: Zhang, Xuehong IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 99 – Type: issue Value: 19 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
