Functionalization of poly-L-lactic-co-ε-caprolactone: effects of surface modification on endothelial cell proliferation and hemocompatibility.
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| Title: | Functionalization of poly-L-lactic-co-ε-caprolactone: effects of surface modification on endothelial cell proliferation and hemocompatibility. |
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| Authors: | Perego, Gabriele1, Preda, Paola2, Pasquinelli, Gianandrea2, Curti, Tiziano3, Freyrie, Antonio3, Cenni, Elisabetta4 elisabetta.cenni@ior.it |
| Source: | Journal of Biomaterials Science -- Polymer Edition. Oct2003, Vol. 14 Issue 10, p1057. 19p. |
| Subjects: | Copolymers, Cell proliferation, Vascular grafts |
| Abstract: | A copolymer of L-lactic acid and ε-caprolactone (PLLACL) was synthesized with the aim of preparing a bioartificial, small-diameter and partially resorbable vascular graft. The material was submitted to surface functionalizations (i.e. chemical modification by means of hydrolytic 'etching' and plasma discharge) to promote endothelial cell (EC) adhesion and growth avoiding platelet adhesion or coagulation factor absorption. Furthermore, the behaviour of human microvascular endothelial cells (HMVEC) seeded on the untreated and treated copolymer is described, as well as the platelet adhesion and the modifications of coagulation factors determined by the copolymer itself. PLLACL in its native state provided little support for EC adhesion. Improved EC adherence was obtained when functional groups were provided on the polymer surface by surface chemical hydrolysis. HMVEC seeded and cultured on the polymer surface did not show any ultrastructural alteration, thus demonstrating the absence of the polymer cytotoxicity. Moreover, SEM analysis performed on cold plasma modified specimens showed the presence of a subconfluent monolayer of EC, with an elongated spread morphology. Both the untreated and treated copolymers induced only slight variations of platelet number, but determined the activated partial thromboplastin time (APTT) increase, due to factor XI reduction. Finally,a prototype of partially biodegradable vascular prosthesis was prepared with NaOH/HCl-treated copolymer. Pre-cultured HMVEC seeding of the prosthesis by means of a rotation device resulted in an almost completely coverage of the graft inner surface. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biomaterials Science -- Polymer Edition is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 10937261 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Functionalization of poly-L-lactic-co-ε-caprolactone: effects of surface modification on endothelial cell proliferation and hemocompatibility. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Perego%2C+Gabriele%22">Perego, Gabriele</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Preda%2C+Paola%22">Preda, Paola</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Pasquinelli%2C+Gianandrea%22">Pasquinelli, Gianandrea</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Curti%2C+Tiziano%22">Curti, Tiziano</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Freyrie%2C+Antonio%22">Freyrie, Antonio</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Cenni%2C+Elisabetta%22">Cenni, Elisabetta</searchLink><relatesTo>4</relatesTo><i> elisabetta.cenni@ior.it</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomaterials+Science+--+Polymer+Edition%22">Journal of Biomaterials Science -- Polymer Edition</searchLink>. Oct2003, Vol. 14 Issue 10, p1057. 19p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Copolymers%22">Copolymers</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Vascular+grafts%22">Vascular grafts</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: A copolymer of L-lactic acid and ε-caprolactone (PLLACL) was synthesized with the aim of preparing a bioartificial, small-diameter and partially resorbable vascular graft. The material was submitted to surface functionalizations (i.e. chemical modification by means of hydrolytic 'etching' and plasma discharge) to promote endothelial cell (EC) adhesion and growth avoiding platelet adhesion or coagulation factor absorption. Furthermore, the behaviour of human microvascular endothelial cells (HMVEC) seeded on the untreated and treated copolymer is described, as well as the platelet adhesion and the modifications of coagulation factors determined by the copolymer itself. PLLACL in its native state provided little support for EC adhesion. Improved EC adherence was obtained when functional groups were provided on the polymer surface by surface chemical hydrolysis. HMVEC seeded and cultured on the polymer surface did not show any ultrastructural alteration, thus demonstrating the absence of the polymer cytotoxicity. Moreover, SEM analysis performed on cold plasma modified specimens showed the presence of a subconfluent monolayer of EC, with an elongated spread morphology. Both the untreated and treated copolymers induced only slight variations of platelet number, but determined the activated partial thromboplastin time (APTT) increase, due to factor XI reduction. Finally,a prototype of partially biodegradable vascular prosthesis was prepared with NaOH/HCl-treated copolymer. Pre-cultured HMVEC seeding of the prosthesis by means of a rotation device resulted in an almost completely coverage of the graft inner surface. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biomaterials Science -- Polymer Edition is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1163/156856203769231565 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 1057 Subjects: – SubjectFull: Copolymers Type: general – SubjectFull: Cell proliferation Type: general – SubjectFull: Vascular grafts Type: general Titles: – TitleFull: Functionalization of poly-L-lactic-co-ε-caprolactone: effects of surface modification on endothelial cell proliferation and hemocompatibility. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Perego, Gabriele – PersonEntity: Name: NameFull: Preda, Paola – PersonEntity: Name: NameFull: Pasquinelli, Gianandrea – PersonEntity: Name: NameFull: Curti, Tiziano – PersonEntity: Name: NameFull: Freyrie, Antonio – PersonEntity: Name: NameFull: Cenni, Elisabetta IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2003 Type: published Y: 2003 Identifiers: – Type: issn-print Value: 09205063 Numbering: – Type: volume Value: 14 – Type: issue Value: 10 Titles: – TitleFull: Journal of Biomaterials Science -- Polymer Edition Type: main |
| ResultId | 1 |
